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- W2106068051 abstract "The properties of voltage dependent Ca2+ current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c-Kit positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times min−1, respectively, in the presence of 1 μM nifedipine. Single ICC-MY isolated by enzyme treatment also showed c-Kit immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under K+-rich pipette solution. The voltage clamp experiments revealed the amplitude of – 329 pA inward current at irregular frequency. With Cs+-rich pipette solution at Vh=−80 mV, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine (1 μM) blocked VDIC. Therefore, we successfully isolated c-Kit positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent Ca2+ currents (VDCCL)." @default.
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- W2106068051 date "2008-01-01" @default.
- W2106068051 modified "2023-10-02" @default.
- W2106068051 title "Voltage-dependent Ca2+Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach" @default.
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- W2106068051 doi "https://doi.org/10.4196/kjpp.2008.12.6.323" @default.
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