FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106087641> ?p ?o ?g. }

• W2106087641 endingPage "269" @default.
• W2106087641 startingPage "251" @default.
• W2106087641 abstract "The Saccharomyces cerevisiae mating pheromone a-factor is a prenylated and carboxyl methylated extracellular peptide signaling molecule. Biogenesis of the a-factor precursor proceeds via a distinctive multistep pathway that involves COOH-terminal modification. NH2-terminal proteolysis, and a nonclassical export mechanism. In this study, we examine the formation and fate of a-factor biosynthetic intermediates to more precisely define the events that occur during a-factor biogenesis. We have identified four distinct a-factor biosynthetic intermediates (P0, P1, P2, and M) by metabolic labeling, immunoprecipitation, and SDS-PAGE. We determined the biochemical composition of each by defining their NH2-terminal amino acid and COOH-terminal modification status. Unexpectedly, we discovered that not one, but two NH2-terminal cleavage steps occur during the biogenesis of a-factor. In addition, we have shown that COOH-terminal prenylation is required for the NH2-terminal processing of a-factor and that all the prenylated a-factor intermediates (P1, P2, and M) are membrane bound, suggesting that many steps of a-factor biogenesis occur in association with membranes. We also observed that although the biogenesis of a-factor is a rapid process, it is inherently inefficient, perhaps reflecting the potential for regulation. Previous studies have identified gene products that participate in the COOH-terminal modification (Ram1p, Ram2p, Ste14p), NH2-terminal processing (Ste24p, Axl1p), and export (Ste6p) of a-factor. The intermediates defined in the present study are discussed in the context of these biogenesis components to formulate an overall model for the pathway of a-factor biogenesis." @default.
• W2106087641 created "2016-06-24" @default.
• W2106087641 creator A5005551657 @default.
• W2106087641 creator A5013652689 @default.
• W2106087641 creator A5036040264 @default.
• W2106087641 creator A5037415815 @default.
• W2106087641 date "1997-01-27" @default.
• W2106087641 modified "2023-10-11" @default.
• W2106087641 title "Biogenesis of the Saccharomyces cerevisiae Mating Pheromone a-Factor" @default.
• W2106087641 cites W131157789 @default.
• W2106087641 cites W1411675973 @default.
• W2106087641 cites W1517380923 @default.
• W2106087641 cites W1527774170 @default.
• W2106087641 cites W1532372971 @default.
• W2106087641 cites W1580812515 @default.
• W2106087641 cites W1599809900 @default.
• W2106087641 cites W1605985759 @default.
• W2106087641 cites W1608409978 @default.
• W2106087641 cites W1822657198 @default.
• W2106087641 cites W1854115061 @default.
• W2106087641 cites W189969322 @default.
• W2106087641 cites W1981655068 @default.
• W2106087641 cites W1988741974 @default.
• W2106087641 cites W1989789175 @default.
• W2106087641 cites W2002544784 @default.
• W2106087641 cites W2003267467 @default.
• W2106087641 cites W2005173649 @default.
• W2106087641 cites W2010207338 @default.
• W2106087641 cites W2018289835 @default.
• W2106087641 cites W2018441710 @default.
• W2106087641 cites W2031168303 @default.
• W2106087641 cites W2032130335 @default.
• W2106087641 cites W2036546637 @default.
• W2106087641 cites W2041834434 @default.
• W2106087641 cites W2048500913 @default.
• W2106087641 cites W2051143903 @default.
• W2106087641 cites W20741063 @default.
• W2106087641 cites W2082064626 @default.
• W2106087641 cites W2083848684 @default.
• W2106087641 cites W2085058883 @default.
• W2106087641 cites W2088693217 @default.
• W2106087641 cites W2091259347 @default.
• W2106087641 cites W2091512138 @default.
• W2106087641 cites W2096892424 @default.
• W2106087641 cites W2100837269 @default.
• W2106087641 cites W2102470371 @default.
• W2106087641 cites W2107360946 @default.
• W2106087641 cites W2112996671 @default.
• W2106087641 cites W2118234018 @default.
• W2106087641 cites W2120875352 @default.
• W2106087641 cites W2124879478 @default.
• W2106087641 cites W2128381266 @default.
• W2106087641 cites W2132417094 @default.
• W2106087641 cites W2143963878 @default.
• W2106087641 cites W2147716555 @default.
• W2106087641 cites W2149912091 @default.
• W2106087641 cites W2151206849 @default.
• W2106087641 cites W2153825396 @default.
• W2106087641 cites W2158407439 @default.
• W2106087641 cites W2160107586 @default.
• W2106087641 cites W2165870010 @default.
• W2106087641 cites W2167960257 @default.
• W2106087641 cites W2168267287 @default.
• W2106087641 cites W2169333620 @default.
• W2106087641 cites W2169446484 @default.
• W2106087641 cites W2176744910 @default.
• W2106087641 cites W2178557511 @default.
• W2106087641 cites W2239824161 @default.
• W2106087641 cites W2319504481 @default.
• W2106087641 cites W23714381 @default.
• W2106087641 cites W250613533 @default.
• W2106087641 cites W274783243 @default.
• W2106087641 cites W2888511473 @default.
• W2106087641 cites W4231617037 @default.
• W2106087641 cites W4236860969 @default.
• W2106087641 cites W70864286 @default.
• W2106087641 doi "https://doi.org/10.1083/jcb.136.2.251" @default.
• W2106087641 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2134810" @default.
• W2106087641 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9015298" @default.
• W2106087641 hasPublicationYear "1997" @default.
• W2106087641 type Work @default.
• W2106087641 sameAs 2106087641 @default.
• W2106087641 citedByCount "122" @default.
• W2106087641 countsByYear W21060876412012 @default.
• W2106087641 countsByYear W21060876412013 @default.
• W2106087641 countsByYear W21060876412014 @default.
• W2106087641 countsByYear W21060876412015 @default.
• W2106087641 countsByYear W21060876412016 @default.
• W2106087641 countsByYear W21060876412018 @default.
• W2106087641 countsByYear W21060876412019 @default.
• W2106087641 countsByYear W21060876412020 @default.
• W2106087641 countsByYear W21060876412021 @default.
• W2106087641 countsByYear W21060876412022 @default.
• W2106087641 countsByYear W21060876412023 @default.
• W2106087641 crossrefType "journal-article" @default.
• W2106087641 hasAuthorship W2106087641A5005551657 @default.
• W2106087641 hasAuthorship W2106087641A5013652689 @default.
• W2106087641 hasAuthorship W2106087641A5036040264 @default.

• next