FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106118844> ?p ?o ?g. }

• W2106118844 endingPage "5293" @default.
• W2106118844 startingPage "5277" @default.
• W2106118844 abstract "ABSTRACT Previously, RNA transcripts of cDNA clones of hepatitis C virus (HCV) genotypes 1a (strains H77, HCV-1, and HC-TN), 1b (HC-J4, Con1, and HCV-N), and 2a (HC-J6 and JFH1) were found to be infectious in chimpanzees. However, only JFH1 was infectious in human hepatoma Huh7 cells. We performed genetic analysis of HCV genotype 3a (strain S52) and 4a (strain ED43) prototype strains and generated full-length consensus cDNA clones (pS52 and pED43). Transfection of Huh7.5 cells with RNA transcripts of these clones did not yield cells expressing HCV Core. However, intrahepatic transfection of chimpanzees resulted in robust infection with peak HCV RNA titers of ∼5.5 log 10 international units (IU)/ml. Genomic consensus sequences recovered from serum at the times of peak viral titers were identical to the sequences of the parental plasmids. Both chimpanzees developed acute hepatitis with elevated liver enzymes and significant necroinflammatory liver changes coinciding with detection of gamma interferon-secreting, intrahepatic T cells. However, the onset and broadness of intrahepatic T-cell responses varied greatly in the two animals, with an early (week 4) multispecific response in the ED43-infected animal (3 weeks before the first evidence of viral control) and a late (week 11) response with limited breadth in the S52-infected animal (without evidence of viral control). Autologous serum neutralizing antibodies were not detected during the acute infection in either animal. Both animals became persistently infected. In conclusion, we generated fully functional infectious cDNA clones of HCV genotypes 3a and 4a. Proof of functionality of all genes might further the development of recombinant cell culture systems for these important genotypes." @default.
• W2106118844 created "2016-06-24" @default.
• W2106118844 creator A5000059912 @default.
• W2106118844 creator A5006685377 @default.
• W2106118844 creator A5007812217 @default.
• W2106118844 creator A5018051199 @default.
• W2106118844 creator A5038234181 @default.
• W2106118844 creator A5042963735 @default.
• W2106118844 creator A5055530758 @default.
• W2106118844 creator A5057120979 @default.
• W2106118844 creator A5066896324 @default.
• W2106118844 creator A5073835639 @default.
• W2106118844 creator A5088076863 @default.
• W2106118844 date "2010-05-15" @default.
• W2106118844 modified "2023-10-11" @default.
• W2106118844 title "Novel Infectious cDNA Clones of Hepatitis C Virus Genotype 3a (Strain S52) and 4a (Strain ED43): Genetic Analyses and <i>In Vivo</i> Pathogenesis Studies" @default.
• W2106118844 cites W1583287101 @default.
• W2106118844 cites W1914884504 @default.
• W2106118844 cites W1963968359 @default.
• W2106118844 cites W1968899686 @default.
• W2106118844 cites W1971505503 @default.
• W2106118844 cites W1974655988 @default.
• W2106118844 cites W1976844675 @default.
• W2106118844 cites W1978201732 @default.
• W2106118844 cites W1985680307 @default.
• W2106118844 cites W1985982900 @default.
• W2106118844 cites W1990058709 @default.
• W2106118844 cites W1992649586 @default.
• W2106118844 cites W1992676108 @default.
• W2106118844 cites W1993091527 @default.
• W2106118844 cites W1993690048 @default.
• W2106118844 cites W1996810729 @default.
• W2106118844 cites W2001241073 @default.
• W2106118844 cites W2001467294 @default.
• W2106118844 cites W2002468303 @default.
• W2106118844 cites W2003579943 @default.
• W2106118844 cites W2010251227 @default.
• W2106118844 cites W2013418738 @default.
• W2106118844 cites W2017359325 @default.
• W2106118844 cites W2017627871 @default.
• W2106118844 cites W2021535457 @default.
• W2106118844 cites W2022687771 @default.
• W2106118844 cites W2023645777 @default.
• W2106118844 cites W2028194374 @default.
• W2106118844 cites W2029323175 @default.
• W2106118844 cites W2030528535 @default.
• W2106118844 cites W2032324783 @default.
• W2106118844 cites W2033125798 @default.
• W2106118844 cites W2035247436 @default.
• W2106118844 cites W2039075777 @default.
• W2106118844 cites W2044193462 @default.
• W2106118844 cites W2045562270 @default.
• W2106118844 cites W2047988967 @default.
• W2106118844 cites W2049016083 @default.
• W2106118844 cites W2050466666 @default.
• W2106118844 cites W2052325775 @default.
• W2106118844 cites W2053536599 @default.
• W2106118844 cites W2062848152 @default.
• W2106118844 cites W2071267501 @default.
• W2106118844 cites W2071837434 @default.
• W2106118844 cites W2072987489 @default.
• W2106118844 cites W2077103006 @default.
• W2106118844 cites W2082220186 @default.
• W2106118844 cites W2090297731 @default.
• W2106118844 cites W2090328228 @default.
• W2106118844 cites W2096779842 @default.
• W2106118844 cites W2097224767 @default.
• W2106118844 cites W2101801554 @default.
• W2106118844 cites W2101832306 @default.
• W2106118844 cites W2102955693 @default.
• W2106118844 cites W2105281461 @default.
• W2106118844 cites W2111359040 @default.
• W2106118844 cites W2113710797 @default.
• W2106118844 cites W2116694893 @default.
• W2106118844 cites W2117184091 @default.
• W2106118844 cites W2119303435 @default.
• W2106118844 cites W2122987848 @default.
• W2106118844 cites W2126482091 @default.
• W2106118844 cites W2127435247 @default.
• W2106118844 cites W2127685726 @default.
• W2106118844 cites W2128549142 @default.
• W2106118844 cites W2130259551 @default.
• W2106118844 cites W2131143399 @default.
• W2106118844 cites W2131605289 @default.
• W2106118844 cites W2133270062 @default.
• W2106118844 cites W2137929296 @default.
• W2106118844 cites W2139054265 @default.
• W2106118844 cites W2139373256 @default.
• W2106118844 cites W2141200498 @default.
• W2106118844 cites W2144361201 @default.
• W2106118844 cites W2146782236 @default.
• W2106118844 cites W2147126871 @default.
• W2106118844 cites W2148099428 @default.
• W2106118844 cites W2148752834 @default.
• W2106118844 cites W2149432056 @default.
• W2106118844 cites W2151898297 @default.
• W2106118844 cites W2155612894 @default.
• W2106118844 cites W2159974321 @default.

• next