Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106142821> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2106142821 abstract "BackgroundWith an effective vaccine being sought for the prevention of HIV transmission, current focus is upon a mucosal vaccine strategy providing protection at the viral point of entry. The full potential of these vaccines can only be realised with the development of successful delivery systems which are also capable of providing an environment conducive to antigen stability. Previously, rheologically structured vehicles (RSVs) were formulated to include therecombinant C-clade HIV-1 envelope protein CN54gp140, and intra-vaginal immunisation in rabbits induced significant systemic IgG and specific IgG and IgA in genital tract secretions. However, CN54gp140 stability within these aqueous formulations remains a concern. Here we investigate the formulation of CN54gp140 within modified lyophilised RSV matrices as a means of providing enhanced protein stabilisation.MethodsRSV formulations were prepared comprising mucoadhesive (polycarbophil, sodium carboxymethylcellulose) and vaginal fluid absorbing (polyvinylpyrrolidine) components. Using rheological analyses in combination with dispensing studies, formulations having appropriate flow properties were chosen for lyophilisation. In vitro release testing of the lyophilised CN54gp140 formulations were investigated in PBST, and following prolonged storage at 37°C the antigenicity of CN54gp140 from the lyophilisedRSVs were analysed by ELISA. The antigen CN54gp140 stability profiles of these formulations were compared to the original aqueous RSV.ResultsCN54gp140 was found to be uniformly distributed within the lyophilised products and exhibited a sustained release profile over an 8 hour period during which the lyophilised 'tablet' underwent complete dissolution in thesurrounding media. While recovery of CN54gp140 from the aqueous RSV diminished over 9 days, lyophilisationshowed no loss in CN54gp140 antigenicity over 67 days storage.ConclusionFormulation of CN54gp140 within a lyophilised gel matrix is shown to have a stabilising effect on the antigen while preserving its sustained release capacity. Such a mucosal vaccine delivery system may offer numerous advantages including ease of use, long-term stability and cold chain avoidance." @default.
- W2106142821 created "2016-06-24" @default.
- W2106142821 creator A5004794046 @default.
- W2106142821 creator A5008667087 @default.
- W2106142821 creator A5018760410 @default.
- W2106142821 creator A5030096380 @default.
- W2106142821 creator A5051168977 @default.
- W2106142821 creator A5060559634 @default.
- W2106142821 creator A5071797512 @default.
- W2106142821 creator A5089256718 @default.
- W2106142821 date "2009-10-01" @default.
- W2106142821 modified "2023-09-27" @default.
- W2106142821 title "P11-11. Stable lyophilised gel vehicles for vaginal administration of recombinant C-clade HIV-1 trimeric CN54gp140" @default.
- W2106142821 doi "https://doi.org/10.1186/1742-4690-6-s3-p156" @default.
- W2106142821 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2767644" @default.
- W2106142821 hasPublicationYear "2009" @default.
- W2106142821 type Work @default.
- W2106142821 sameAs 2106142821 @default.
- W2106142821 citedByCount "0" @default.
- W2106142821 crossrefType "journal-article" @default.
- W2106142821 hasAuthorship W2106142821A5004794046 @default.
- W2106142821 hasAuthorship W2106142821A5008667087 @default.
- W2106142821 hasAuthorship W2106142821A5018760410 @default.
- W2106142821 hasAuthorship W2106142821A5030096380 @default.
- W2106142821 hasAuthorship W2106142821A5051168977 @default.
- W2106142821 hasAuthorship W2106142821A5060559634 @default.
- W2106142821 hasAuthorship W2106142821A5071797512 @default.
- W2106142821 hasAuthorship W2106142821A5089256718 @default.
- W2106142821 hasBestOaLocation W21061428211 @default.
- W2106142821 hasConcept C104317684 @default.
- W2106142821 hasConcept C159047783 @default.
- W2106142821 hasConcept C3013748606 @default.
- W2106142821 hasConcept C40767141 @default.
- W2106142821 hasConcept C44465124 @default.
- W2106142821 hasConcept C55493867 @default.
- W2106142821 hasConcept C71924100 @default.
- W2106142821 hasConcept C86803240 @default.
- W2106142821 hasConcept C90132467 @default.
- W2106142821 hasConceptScore W2106142821C104317684 @default.
- W2106142821 hasConceptScore W2106142821C159047783 @default.
- W2106142821 hasConceptScore W2106142821C3013748606 @default.
- W2106142821 hasConceptScore W2106142821C40767141 @default.
- W2106142821 hasConceptScore W2106142821C44465124 @default.
- W2106142821 hasConceptScore W2106142821C55493867 @default.
- W2106142821 hasConceptScore W2106142821C71924100 @default.
- W2106142821 hasConceptScore W2106142821C86803240 @default.
- W2106142821 hasConceptScore W2106142821C90132467 @default.
- W2106142821 hasIssue "S3" @default.
- W2106142821 hasLocation W21061428211 @default.
- W2106142821 hasLocation W21061428212 @default.
- W2106142821 hasLocation W21061428213 @default.
- W2106142821 hasLocation W21061428214 @default.
- W2106142821 hasOpenAccess W2106142821 @default.
- W2106142821 hasPrimaryLocation W21061428211 @default.
- W2106142821 hasRelatedWork W1973204141 @default.
- W2106142821 hasRelatedWork W1975200898 @default.
- W2106142821 hasRelatedWork W2008418219 @default.
- W2106142821 hasRelatedWork W2039849534 @default.
- W2106142821 hasRelatedWork W2380729652 @default.
- W2106142821 hasRelatedWork W2392849191 @default.
- W2106142821 hasRelatedWork W2394247790 @default.
- W2106142821 hasRelatedWork W3038839440 @default.
- W2106142821 hasRelatedWork W4300011733 @default.
- W2106142821 hasRelatedWork W4307619433 @default.
- W2106142821 hasVolume "6" @default.
- W2106142821 isParatext "false" @default.
- W2106142821 isRetracted "false" @default.
- W2106142821 magId "2106142821" @default.
- W2106142821 workType "article" @default.