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• W2106184802 abstract "Intracellular Mg2+ and natural polyamines block outward currents in BK channels in a highly voltage-dependent manner. Here we investigate the contribution of the ring of eight negatively charged residues (4 x E321/E324) at the entrance to the inner vestibule of BK channels to this block. Channels with or without (E321N/E324N) the ring of negative charge were expressed in oocytes and unitary currents were recorded from inside-out patches over a range of intracellular Mg2+ and polyamine concentrations. Removing the ring of charge greatly decreased the block, increasing KBap (0 mV) for Mg2+ block from 48.3 ± 3.0 to 143 ± 8 mM, and for spermine block from 8.0 ± 1.0 to 721 ± 9 mM (150 mM symmetrical KCl). Polyamines with fewer amine groups blocked less: putrescine &lt; spermidine &lt; spermine. An equation that combined an empirical Hill function for block together with a Boltzmann function for the voltage dependence of KBap described the voltage and concentration dependence of the block for channels with and without the ring of charge. The Hill coefficients for these descriptions were &lt;1 for both Mg2+ and spermine block, and were unchanged by removing the ring of charge. When KCli was increased from 150 mM to 3 M, the ring of charge no longer facilitated block, Mg2+ block was reduced, spermine block became negligible, and the Hill coefficients became ∼1.0. BK channels in cell-attached oocyte patches displayed inward rectification, which was reduced for channels without the ring of charge. Taken together, these observations suggest that the ring of negative charge facilitates block through a preferential electrostatic attraction of Mg2+ and polyamine over K+. This preferential attraction of multivalent blockers over monovalent K+ would decrease the K+ available at the inner vestibule to carry outward current in the presence of Mg2+ or polyamines, while increasing the concentration of blocker available to enter and block the conduction pathway." @default.
• W2106184802 created "2016-06-24" @default.
• W2106184802 creator A5024563064 @default.
• W2106184802 creator A5042978201 @default.
• W2106184802 creator A5048680925 @default.
• W2106184802 creator A5065649112 @default.
• W2106184802 date "2006-07-17" @default.
• W2106184802 modified "2023-10-16" @default.
• W2106184802 title "Ring of Negative Charge in BK Channels Facilitates Block by Intracellular Mg2+ and Polyamines through Electrostatics" @default.
• W2106184802 cites W1519998997 @default.
• W2106184802 cites W1533488243 @default.
• W2106184802 cites W1972704395 @default.
• W2106184802 cites W1974558586 @default.
• W2106184802 cites W1975201915 @default.
• W2106184802 cites W1977127647 @default.
• W2106184802 cites W1977221331 @default.
• W2106184802 cites W1979856671 @default.
• W2106184802 cites W1980257254 @default.
• W2106184802 cites W1980314278 @default.
• W2106184802 cites W1986644095 @default.
• W2106184802 cites W1986740737 @default.
• W2106184802 cites W1991357993 @default.
• W2106184802 cites W1997511216 @default.
• W2106184802 cites W1997883766 @default.
• W2106184802 cites W2003049938 @default.
• W2106184802 cites W2004079359 @default.
• W2106184802 cites W2004706690 @default.
• W2106184802 cites W2004868309 @default.
• W2106184802 cites W2006090981 @default.
• W2106184802 cites W2009667219 @default.
• W2106184802 cites W2010395609 @default.
• W2106184802 cites W2014899976 @default.
• W2106184802 cites W2015426350 @default.
• W2106184802 cites W2015841601 @default.
• W2106184802 cites W2017450269 @default.
• W2106184802 cites W2018083776 @default.
• W2106184802 cites W2024702471 @default.
• W2106184802 cites W2026846610 @default.
• W2106184802 cites W2037539407 @default.
• W2106184802 cites W2038827150 @default.
• W2106184802 cites W2041845061 @default.
• W2106184802 cites W2044813037 @default.
• W2106184802 cites W2045069432 @default.
• W2106184802 cites W2046829326 @default.
• W2106184802 cites W2047803825 @default.
• W2106184802 cites W2051572960 @default.
• W2106184802 cites W2055252729 @default.
• W2106184802 cites W2056196705 @default.
• W2106184802 cites W2058612305 @default.
• W2106184802 cites W2063048094 @default.
• W2106184802 cites W2074220951 @default.
• W2106184802 cites W2076832064 @default.
• W2106184802 cites W2078747669 @default.
• W2106184802 cites W2080082506 @default.
• W2106184802 cites W2085320910 @default.
• W2106184802 cites W2090677452 @default.
• W2106184802 cites W2096891609 @default.
• W2106184802 cites W2098105911 @default.
• W2106184802 cites W2099975374 @default.
• W2106184802 cites W2101204196 @default.
• W2106184802 cites W2101753034 @default.
• W2106184802 cites W2106207499 @default.
• W2106184802 cites W2107320528 @default.
• W2106184802 cites W2108285364 @default.
• W2106184802 cites W2113312025 @default.
• W2106184802 cites W2116242834 @default.
• W2106184802 cites W2116249778 @default.
• W2106184802 cites W2118298051 @default.
• W2106184802 cites W2118516008 @default.
• W2106184802 cites W2124638070 @default.
• W2106184802 cites W2125199990 @default.
• W2106184802 cites W2127000342 @default.
• W2106184802 cites W2129646250 @default.
• W2106184802 cites W2133464980 @default.
• W2106184802 cites W2134933577 @default.
• W2106184802 cites W2135440354 @default.
• W2106184802 cites W2137288855 @default.
• W2106184802 cites W2141494494 @default.
• W2106184802 cites W2141920387 @default.
• W2106184802 cites W2143070723 @default.
• W2106184802 cites W2143654805 @default.
• W2106184802 cites W2148223457 @default.
• W2106184802 cites W2151791892 @default.
• W2106184802 cites W2154525124 @default.
• W2106184802 cites W2156677955 @default.
• W2106184802 cites W2158409803 @default.
• W2106184802 cites W2162547857 @default.
• W2106184802 cites W2168230176 @default.
• W2106184802 cites W2218986966 @default.
• W2106184802 cites W2379125756 @default.
• W2106184802 cites W41069508 @default.
• W2106184802 cites W959407442 @default.
• W2106184802 doi "https://doi.org/10.1085/jgp.200609493" @default.
• W2106184802 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2151526" @default.
• W2106184802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16847096" @default.
• W2106184802 hasPublicationYear "2006" @default.
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