FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106208801> ?p ?o ?g. }

• W2106208801 endingPage "1160" @default.
• W2106208801 startingPage "1151" @default.
• W2106208801 abstract "Shiga toxin of Shigella dysenteriae type I and Shiga-like toxins I and II (SLT-I and SLT-II, respectively) of enterohemorrhagic Escherichia coli are functionally similar protein cytotoxins. These toxin molecules have a bipartite molecular structure which consists of an enzymatically active A subunit that inhibits protein synthesis in eukaryotic cells and an oligomeric B subunit that binds to globotriaosylceramide glycolipid receptors on eukaryotic cells. Regionally directed chemical mutagenesis of the B subunit of SLT-II was used to identify amino acids in the B subunit that are critical for SLT-II holotoxin cytotoxic activity. Three noncytotoxic mutants were isolated, and their mutations were mapped. The substitutions of arginine with cysteine at codon 32, alanine with threonine at codon 42, and glycine with aspartic acid at codon 59 in the 70-amino-acid mature SLT-II B polypeptide resulted in the complete abolition of cytotoxicity. The analogous arginine, alanine, and glycine residues were conserved at codons 33, 43, and 60 in the 69-amino-acid mature B polypeptide of Shiga toxin. Comparable mutations induced in the B-subunit gene of Shiga toxin by oligonucleotide-directed, site-specific mutagenesis resulted in drastically decreased cytotoxicity (10(3)- to 10(6)-fold) as compared with that of wild-type Shiga toxin. The mutant SLT-II and Shiga toxin B subunits were characterized for stability, receptor binding, immunoreactivity, and ability to be assembled into holotoxin." @default.
• W2106208801 created "2016-06-24" @default.
• W2106208801 creator A5044328066 @default.
• W2106208801 creator A5044549524 @default.
• W2106208801 creator A5047900679 @default.
• W2106208801 creator A5061880309 @default.
• W2106208801 date "1991-02-01" @default.
• W2106208801 modified "2023-10-16" @default.
• W2106208801 title "Identification of three amino acid residues in the B subunit of Shiga toxin and Shiga-like toxin type II that are essential for holotoxin activity" @default.
• W2106208801 cites W1444072294 @default.
• W2106208801 cites W1482533589 @default.
• W2106208801 cites W1492813001 @default.
• W2106208801 cites W1505020864 @default.
• W2106208801 cites W1512557372 @default.
• W2106208801 cites W1534382357 @default.
• W2106208801 cites W1548102615 @default.
• W2106208801 cites W1587924364 @default.
• W2106208801 cites W1641182372 @default.
• W2106208801 cites W1708081159 @default.
• W2106208801 cites W1749990518 @default.
• W2106208801 cites W1922154279 @default.
• W2106208801 cites W1942715185 @default.
• W2106208801 cites W1955247866 @default.
• W2106208801 cites W1959629924 @default.
• W2106208801 cites W1972168834 @default.
• W2106208801 cites W1991221729 @default.
• W2106208801 cites W1996050139 @default.
• W2106208801 cites W2019626758 @default.
• W2106208801 cites W2023731533 @default.
• W2106208801 cites W2026435853 @default.
• W2106208801 cites W2040459802 @default.
• W2106208801 cites W2066018712 @default.
• W2106208801 cites W2074484003 @default.
• W2106208801 cites W2101170621 @default.
• W2106208801 cites W2113864538 @default.
• W2106208801 cites W2163744073 @default.
• W2106208801 cites W2166761865 @default.
• W2106208801 cites W2167223768 @default.
• W2106208801 cites W2334020175 @default.
• W2106208801 cites W2888511473 @default.
• W2106208801 doi "https://doi.org/10.1128/jb.173.3.1151-1160.1991" @default.
• W2106208801 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/207236" @default.
• W2106208801 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1991714" @default.
• W2106208801 hasPublicationYear "1991" @default.
• W2106208801 type Work @default.
• W2106208801 sameAs 2106208801 @default.
• W2106208801 citedByCount "26" @default.
• W2106208801 countsByYear W21062088012012 @default.
• W2106208801 countsByYear W21062088012014 @default.
• W2106208801 countsByYear W21062088012015 @default.
• W2106208801 countsByYear W21062088012016 @default.
• W2106208801 countsByYear W21062088012020 @default.
• W2106208801 crossrefType "journal-article" @default.
• W2106208801 hasAuthorship W2106208801A5044328066 @default.
• W2106208801 hasAuthorship W2106208801A5044549524 @default.
• W2106208801 hasAuthorship W2106208801A5047900679 @default.
• W2106208801 hasAuthorship W2106208801A5061880309 @default.
• W2106208801 hasBestOaLocation W21062088011 @default.
• W2106208801 hasConcept C104292427 @default.
• W2106208801 hasConcept C104317684 @default.
• W2106208801 hasConcept C143065580 @default.
• W2106208801 hasConcept C153911025 @default.
• W2106208801 hasConcept C16318435 @default.
• W2106208801 hasConcept C2777367657 @default.
• W2106208801 hasConcept C2778411228 @default.
• W2106208801 hasConcept C2779856020 @default.
• W2106208801 hasConcept C515207424 @default.
• W2106208801 hasConcept C547475151 @default.
• W2106208801 hasConcept C55493867 @default.
• W2106208801 hasConcept C86803240 @default.
• W2106208801 hasConceptScore W2106208801C104292427 @default.
• W2106208801 hasConceptScore W2106208801C104317684 @default.
• W2106208801 hasConceptScore W2106208801C143065580 @default.
• W2106208801 hasConceptScore W2106208801C153911025 @default.
• W2106208801 hasConceptScore W2106208801C16318435 @default.
• W2106208801 hasConceptScore W2106208801C2777367657 @default.
• W2106208801 hasConceptScore W2106208801C2778411228 @default.
• W2106208801 hasConceptScore W2106208801C2779856020 @default.
• W2106208801 hasConceptScore W2106208801C515207424 @default.
• W2106208801 hasConceptScore W2106208801C547475151 @default.
• W2106208801 hasConceptScore W2106208801C55493867 @default.
• W2106208801 hasConceptScore W2106208801C86803240 @default.
• W2106208801 hasIssue "3" @default.
• W2106208801 hasLocation W21062088011 @default.
• W2106208801 hasLocation W21062088012 @default.
• W2106208801 hasLocation W21062088013 @default.
• W2106208801 hasLocation W21062088014 @default.
• W2106208801 hasOpenAccess W2106208801 @default.
• W2106208801 hasPrimaryLocation W21062088011 @default.
• W2106208801 hasRelatedWork W1482817952 @default.
• W2106208801 hasRelatedWork W1942501778 @default.
• W2106208801 hasRelatedWork W2019576136 @default.
• W2106208801 hasRelatedWork W2058266044 @default.
• W2106208801 hasRelatedWork W2066572513 @default.
• W2106208801 hasRelatedWork W2078718478 @default.
• W2106208801 hasRelatedWork W2134472134 @default.
• W2106208801 hasRelatedWork W2279156773 @default.
• W2106208801 hasRelatedWork W2797991563 @default.

• next