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• W2106341974 abstract "The present study was designed to evaluate the plasticity of mouse embryonic stem cells (ESCs). Cardiac and neural differentiation of the mouse ESCs was initiated by embryoid bodies (EBs) formation in hanging drops and in suspension. After aggregation the were transfered to the suspension culture and then plating on to gelatin-coated tissue culture plates. Retinoic acid was added to culture medium through out the suspension period. Cultures were observed daily withan inverted microscope for the appearance of contracting clusters and neural filaments. At the early,intermediate and terminal stages of differentiation, the choronotropic responses of cardiomyocytes to cardioactive drugs were assessed,and the cardiomyocytes immunostained for citokeratin, titin and betaIIItubulin. Our data suggest that ESCs can differentiate into functional mature cardiomyocytes in vitro. Furthermore, ESC-cardiomyocytes may provide an ideal model for the study of cardiomyocytic development and may be useful for cell therapy of various cardiac diseases." @default.
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• W2106341974 date "2010-01-01" @default.
• W2106341974 modified "2023-10-18" @default.
• W2106341974 title "Evaluation of mouse embryonic stem cells plasticity through different differentiation protocols." @default.
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• W2106341974 doi "https://doi.org/10.15835/buasvmcn-vm:67:2:5929" @default.
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