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• W2106345114 abstract "Alanine-scanning mutagenesis was applied to the ∊ subunit of the F1-F0 ATP synthase from E. coli. Nineteen amino acid residues were changed to alanine, either singly or in pairs, between residues 10 and 93. All mutants, when expressed in the ∊ deletion strain XH1, were able to grow on succinate minimal medium. Membranes were prepared from all mutants and assayed for ATP-driven proton translocation, ATP hydrolysis ± lauryldiethylamine oxide, and sensitivity of ATPase activity to N,N‘-dicyclohexylcarbodiimide (DCCD). Most of the mutants fell into 2 distinct classes. The first group had inhibited ATPase activity, with near normal levels of membrane-bound F1, but decreased sensitivity to DCCD. The second group had stimulated ATPase activity, with a reduced level of membrane-bound F1, but normal sensitivity to DCCD. Membranes from all mutants were further characterized by immunoblotting using 2 monoclonal antibodies. A model for the secondary structure of ∊ and its role in the function of the ATP synthase has been developed. Some residues are important for the binding of ∊ to F1 and therefore for inhibition. Other residues, from Glu-59 through Glu-70, are important for the release of inhibition by ∊ that is part of the normal enzyme cycle. Alanine-scanning mutagenesis was applied to the ∊ subunit of the F1-F0 ATP synthase from E. coli. Nineteen amino acid residues were changed to alanine, either singly or in pairs, between residues 10 and 93. All mutants, when expressed in the ∊ deletion strain XH1, were able to grow on succinate minimal medium. Membranes were prepared from all mutants and assayed for ATP-driven proton translocation, ATP hydrolysis ± lauryldiethylamine oxide, and sensitivity of ATPase activity to N,N‘-dicyclohexylcarbodiimide (DCCD). Most of the mutants fell into 2 distinct classes. The first group had inhibited ATPase activity, with near normal levels of membrane-bound F1, but decreased sensitivity to DCCD. The second group had stimulated ATPase activity, with a reduced level of membrane-bound F1, but normal sensitivity to DCCD. Membranes from all mutants were further characterized by immunoblotting using 2 monoclonal antibodies. A model for the secondary structure of ∊ and its role in the function of the ATP synthase has been developed. Some residues are important for the binding of ∊ to F1 and therefore for inhibition. Other residues, from Glu-59 through Glu-70, are important for the release of inhibition by ∊ that is part of the normal enzyme cycle. INTRODUCTIONThe F1-F0 ATP synthase from Escherichia coli (for reviews, see (1Fillingame R.H. Krulwich T.A. The Bacteria. Academic Press, Inc., New York1990: 345-391Google Scholar, 2Futai M. Noumi T. Maeda M. Annu. Rev. Biochem. 1989; 58: 111-136Crossref PubMed Scopus (403) Google Scholar, 3Senior A.E. Annu. Rev. Biophys. Biophys. Chem. 1990; 19: 7-41Crossref PubMed Scopus (328) Google Scholar)) is composed of eight different types of polypeptide subunits which are coded by eight genes found in a single operon called unc (or atp). The enzyme can be physically separated into a membrane-bound portion called F0 and a water-soluble portion called F1. F1 consists of five types of subunits: α (uncA), β (uncD), γ (uncG), δ (uncH), and ∊ (uncC) in a stoichiometry of 3:3:1:1:1. F0 consists of three types of subunits: a (uncB), b (uncF), and c (uncE) in a stoichiometry of 1:2:10 ± 1 (4Foster D.L. Fillingame R.H. J. Biol. Chem. 1982; 257: 2009-2015Abstract Full Text PDF PubMed Google Scholar).In the intact F1-F0 complex, the net synthesis of ATP at sites in F1 is in response to an electrochemical proton gradient across the membrane, which drives proton movement through F0. This proton movement is coupled to net synthesis of ATP at sites in F1, probably via a series of conformational changes. Isolated F1 catalyzes the hydrolysis of ATP, but not its synthesis. The molecular events linking the movement of protons through the membrane-bound portion of the ATPase to the synthesis of ATP at distant sites, remain essentially unknown. Recently, x-ray crystallographic studies of F1 from bovine mitochondria (5Abrahams J.P. Leslie A.G.W. Lutter R. Walker J.E. Nature. 1994; 370: 621-628Crossref PubMed Scopus (2734) Google Scholar) has provided structural information about α, β, and parts of γ. In the resulting model, the amino and carboxyl termini of γ penetrate the central cavity of the α3β3 hexamer, in an asymmetric fashion. One interpretation is that the γ subunit rotates with respect to α and β, thereby sequentially interacting with different catalytic sites, as indicated by earlier studies(6Gogol E.P. Johnston E. Aggeler R. Capaldi R.A. Proc. Natl. Acad. Sci. U. S. A. 1990; 87: 9585-9589Crossref PubMed Scopus (100) Google Scholar).With the E. coli enzyme, two additional subunits, δ and ∊, are necessary for the proper binding of the α3β3γ complex to F0. In addition, ∊ has the interesting property of acting as an intrinsic inhibitor of ATP hydrolysis by the soluble F1(7Laget P.P. Smith J.B. Arch. Biochem. Biophys. 1979; 197: 83-89Crossref PubMed Scopus (69) Google Scholar, 8Sternweiss P.C. Smith J.B. Biochemistry. 1980; 19: 526-531Crossref PubMed Scopus (106) Google Scholar). The mechanism by which ∊ acts upon the remaining F1 subunits to cause inhibition is unknown. The removal (or displacement) of ∊ from the complex results in about a 6-fold increase in ATP hydrolysis activity(9Lötscher H.R. deJong C. Capaldi R.A. Biochemistry. 1984; 23: 4140-4143Crossref PubMed Scopus (99) Google Scholar, 10Dunn S.D. Zadorozny V.D. Tozer R.G. Orr L.E. Biochemistry. 1987; 26: 4488-4493Crossref PubMed Scopus (63) Google Scholar). This effect is not dependent upon the presence of the δ subunit. The ∊ subunit is thought to inhibit by reducing the rate of product release(10Dunn S.D. Zadorozny V.D. Tozer R.G. Orr L.E. Biochemistry. 1987; 26: 4488-4493Crossref PubMed Scopus (63) Google Scholar), the rate-limiting step in hydrolysis. It is known to bind to isolated γ subunit(11Dunn S.D. J. Biol. Chem. 1982; 257: 7354-7359Abstract Full Text PDF PubMed Google Scholar), but can be cross-linked to α, β, and γ subunits(12Dallmann H.G. Flynn T.G. Dunn S.D. J. Biol. Chem. 1992; 267: 18953-18960Abstract Full Text PDF PubMed Google Scholar, 13Aggeler R. Chicas-Cruz K. Lai S.X. Keana J.F.W. Capaldi R.A. Biochemistry. 1992; 31: 2956-2961Crossref PubMed Scopus (95) Google Scholar, 14Lötscher H.R. deJong C. Capaldi R.A. Biochemistry. 1984; 23: 4134-4140Crossref PubMed Scopus (53) Google Scholar, 15Stan-Lotter H. Bragg P.D. Eur. J. Biochem. 1986; 154: 321-327Crossref PubMed Scopus (19) Google Scholar).A series of deletion mutants has been constructed from the carboxyl terminus of ∊ and analyzed(16Kuki M. Noumi T. Maeda M. Amemura A. Futai M. J. Biol. Chem. 1988; 263: 17437-17442Abstract Full Text PDF PubMed Google Scholar). A truncated form containing only the first 93 residues, plus 2 serine residues at the new carboxyl terminus, was capable of inhibiting ATP hydrolysis and of promoting the binding of F1 to F0. A shorter subunit (80 residues) was also capable of the binding function, but did not have inhibitory properties. These data seem to suggest that an “inhibitory domain” exists, composed at least partially of residues between 80 and 93, and that it is distinct from a binding domain in the first 80 residues. Other studies (17Jounouchi M. Takeyama M. Noumi T. Moriyama Y. Maeda M. Futai M. Arch. Biochem. Biophys. 1992; 292: 87-94Crossref PubMed Scopus (28) Google Scholar, 18Skakoon E.N. Dunn S.D. Arch. Biochem. Biophys. 1993; 302: 279-284Crossref PubMed Scopus (14) Google Scholar) indicated that about 15 amino acids can be lost from the amino terminus without a total loss of function.Nucleotide-dependent conformational changes in ∊ have been detected by protease sensitivity (19Mendel-Hartvig J. Capaldi R.A. Biochemistry. 1991; 30: 1278-1284Crossref PubMed Scopus (71) Google Scholar) and by cryoelectron microscopy using monoclonal antibodies(20Wilkens S. Capaldi R.A. Biol. Chem. Hoppe Seyler. 1994; 375: 43-51Crossref PubMed Scopus (51) Google Scholar). An interesting recent result is that second-site suppressors of a c subunit mutation (Q42E) were mapped to ∊-Glu-31. Quite possibly, both ∊ and γ might be intimately involved in the coupling of proton translocation to ATP synthesis, as has been reviewed recently(21Capaldi R.A. Aggeler R. Turina P. Wilkens S. Trends Biochem. Sci. 1994; 19: 284-289Abstract Full Text PDF PubMed Scopus (130) Google Scholar). To probe the interactions of ∊ with other subunits, and its role in function, we have applied alanine-scanning mutagenesis(22Wells J.A. Methods Enzymol. 1991; 202: 390-411Crossref PubMed Scopus (309) Google Scholar).EXPERIMENTAL PROCEDURESMaterialsRestriction endonucleases and T4 DNA ligase were obtained from New England Biolabs, Inc. Taq DNA polymerase was obtained from Life Technologies, Inc. or from Promega Corp. [α-35S]dATP was obtained from Amersham. Synthetic oligonucleotides (Fig. 1) were obtained from Operon Technologies, Inc. or from National Biosciences, Inc. Urea was from International Biotechnologies, Inc. ATP was obtained from Boehringer Mannheim. Neutralized phenol-chloroform was obtained from Amresco. Promega Corp. supplied Wizard Minipreps for plasmid isolation for sequencing. ACMA 1The abbreviations used are: ACMA9-amino,6-chloro,2-methoxyacridineLDAOlauryldiethylamine oxideDCCDN,N‘-dicyclohexylcarbodiimideMops4-morpholinepropanesulfonic acid. was obtained from Molecular Probes. LDAO and DCCD were obtained from Fluka. Immunoblotting reagents were obtained from Bio-Rad, and anti-∊ monoclonal antibodies, ∊I and ∊II, were a generous gift of Dr. Roderick Capaldi, University of Oregon(23Aggeler R. Mendel-Hartvig J. Capaldi R.A. Biochemistry. 1990; 29: 10387-10393Crossref PubMed Scopus (25) Google Scholar).Bacterial StrainsStrain XL1-Blue (recA1, endA1, gyrA96, thi, hsdR17(rk-, mk+), supE44, relA1, λ-, (lac), (F‘, proAB, lacIq ZΔM15, Tn10(TetR))) was obtained from Stratagene and was used routinely for subclonings and mutagenesis. Strain XH1 (ΔuncC, bglR, thi-1, rel-1, Hfr PO1) (24Xiong H. Vik S.B. J. Bacteriol. 1995; 177: 851-853Crossref PubMed Google Scholar) was used to characterize mutations in uncC. It contains a deletion from −2 to 271 in uncC and produces no detectable ∊ subunit. It is complemented by plasmid pXH302S, which contains a synthetic version of uncC. Cultures were grown at 37°C, and cell density was monitored by optical density at 600 nm using a Milton Roy 1001 spectrophotometer. Rich medium was Luria Broth supplemented with 0.2% glucose, and minimal medium was A salts supplemented with succinate (0.2%) or with glucose, as indicated(25Miller J.H. Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY1972Google Scholar). Media were supplemented with ampicillin (100 mg/liter) or tetracycline (12.5 mg/liter) as appropriate.Construction of MutationsPlasmid pXH302S was used to construct the mutations (shown in Fig. 1) using the cassette mutagenesis technique, as described previously(26Vik S.B. Cain B.D. Chun K.T. Simoni R.D. J. Biol. Chem. 1988; 263: 6599-6605Abstract Full Text PDF PubMed Google Scholar, 27LaRoe D.L. Vik S.B. J. Bacteriol. 1992; 174: 633-637Crossref PubMed Google Scholar). Plasmids were subjected to sequential digestion by each of the two indicated restriction enzymes, followed by ligation with annealed oligonucleotides. Following mutagenesis, plasmids were isolated from transformants (28Sambrook J. Fritsch E.F. Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY1989Google Scholar) and screened by various restriction enzyme digestions to detect the presence of mutations. Subsequently, the DNA sequences of plasmids thought to bear mutations were analyzed using [α-35S]dATP as described before (26Vik S.B. Cain B.D. Chun K.T. Simoni R.D. J. Biol. Chem. 1988; 263: 6599-6605Abstract Full Text PDF PubMed Google Scholar) or by a silver staining technique (Promega) according to the manufacturer's instructions. As indicated in Fig. 1, S10A and F16A were constructed simultaneously in the same plasmid. These mutations were later isolated using a unique SacII restriction site which lies between the two mutations. Most of the other mutations were constructed using degenerate oligonucleotides, which produced mixtures of wild type and mutant residues that were identified after sequence analysis. One mutation, E90A, was never isolated. Mutations were characterized following transformation of the strain XH1.Preparation of Cell Fractions and AssaysFractionation of cells and isolation of membranes and stripped membranes were carried out as described(29Cain B.D. Simoni R.D. J. Biol. Chem. 1988; 263: 6606-6612Abstract Full Text PDF PubMed Google Scholar). Protein concentrations were determined by the Bio-Rad protein assay using bovine serum albumin as standard. ATP hydrolysis assays were carried out as described (9Lötscher H.R. deJong C. Capaldi R.A. Biochemistry. 1984; 23: 4140-4143Crossref PubMed Scopus (99) Google Scholar) with 50 mM Tris acetate, pH 7.5, 3 mM ATP, 5 mM MgCl2, 5 mM KCN, 2 mM phosphoenolpyruvate, 0.25 mM NADH, pyruvate kinase (6 units), and lactate dehydrogenase (8 units) at 37°C using a Beckman DU70 spectrophotometer. For release of inhibition by ∊, LDAO was added to the assay medium to a final concentration of 1%(9Lötscher H.R. deJong C. Capaldi R.A. Biochemistry. 1984; 23: 4140-4143Crossref PubMed Scopus (99) Google Scholar). Inhibition of ATP hydrolysis by DCCD was performed as described previously(26Vik S.B. Cain B.D. Chun K.T. Simoni R.D. J. Biol. Chem. 1988; 263: 6599-6605Abstract Full Text PDF PubMed Google Scholar). Fluorescence quenching assays were performed using 400 μg/ml membrane protein in a solution of 50 mM Mops, pH 7.3, 10 mM MgCl2, 1 μM ACMA, and either 0.5 mM NADH or 0.1 mM ATP, as appropriate. The excitation wavelength was 410 nm and the emission wavelength was 490 nm. Fluorescence quenching was abolished by addition of 1 μM carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone.ImmunoblottingSamples of membranes (75 μg of protein) were electrophoresed and immunoblotted as described previously(30Wang S. Vik S.B. J. Biol. Chem. 1994; 269: 3095-3099Abstract Full Text PDF PubMed Google Scholar), using anti-∊ monoclonal antibodies and goat, anti-mouse IgG-alkaline phosphatase conjugate.RESULTSMutations were constructed as outlined in Fig. 1. Amino acid residues in ∊ were converted to alanine to maximize the possibility that the effects of mutagenesis would be confined to local changes. Residues were selected for mutagenesis based on the following criteria. First, partially conserved, and generally polar, residues were selected. Second, the locations of the mutations were generally confined to the first 85 amino acids, the size of ∊ that was shown to be sufficient for inhibition of F1 ATPase activity(16Kuki M. Noumi T. Maeda M. Amemura A. Futai M. J. Biol. Chem. 1988; 263: 17437-17442Abstract Full Text PDF PubMed Google Scholar).Eighteen mutants, with either single or double alanine substitutions in ∊, were analyzed for growth properties following transformation of the ∊ deletion strain XH1. The mutants that were constructed and their growth properties are shown in Table 1. Only 2 mutants, S10A and F16A, grew poorly on minimal succinate medium, as compared to wild type. The measurement of growth yields in minimal glucose medium confirmed those results: S10A (48%), F16A (67%), T77A (85%), and all of the rest were >90% of the wild type level. The results of ATP-driven proton translocation assays by membrane vesicles followed the same pattern: S10A and F16A had no activity, E70A and T77A had 65% and 60% of the wild type activity, respectively, and all of the rest were >90%.Tabled 1 Open table in a new tab ATP hydrolysis by membrane vesicles from these mutants was measured under three sets of conditions, and the results are shown in Table 1. The standard assay was the coupled enzyme assay at pH 7.5. This assay was repeated in the presence of 1% LDAO, which is known to release the inhibition of F1 ATPase activity by ∊(9Lötscher H.R. deJong C. Capaldi R.A. Biochemistry. 1984; 23: 4140-4143Crossref PubMed Scopus (99) Google Scholar, 31Dunn S.D. Tozer R.G. Zadorozny V.D. Biochemistry. 1990; 29: 4335-4340Crossref PubMed Scopus (53) Google Scholar). The second assay reveals two properties of the mutants. First, it yields the level of uninhibited ATPase activity of the membranes, which reflects the total amount of F1 bound. Second, when compared with the first assay it shows the fold stimulation upon LDAO addition, which reflects the fold inhibition by ∊. Finally, a third assay was performed after preincubation with DCCD, a specific inhibitor of proton translocation by F0. Decreased sensitivity of ATPase activity to DCCD reflects ATP hydrolysis that is not obligatorily coupled to proton translocation.Results of the standard ATP hydrolysis assays revealed that membranes from S10A and F16A had undetectable levels of membrane-bound ATPase. Membranes from the other mutants ranged from 51% to 132% of the wild type levels of ATPase activity, suggesting 2 classes of mutants. This was confirmed by assays in the presence of LDAO, or after preincubation with DCCD. Six mutants showed stimulation of ATP hydrolysis by LDAO of less than 2.4-fold, compared to the wild type value of 3.1-fold. These mutants were T77A, R85A, D81A/R85A, T82A/R85A, T43A, and K46A. This group also tended to have lower levels of bound F1 ATPase, averaging 61% of wild type, and to have normal sensitivity to DCCD, averaging 60% (95% of the wild type value).A second class consisted of E70A, S65A, E59A/E60A, K54A/E60A, E59A, and E31A. These mutants showed higher than normal stimulation of ATPase activity by LDAO, with values ranging from 3.5- to 4.6-fold. These mutants showed a more normal level of membrane-bound F1 ATPase, averaging 85% of the wild type level. However, the membrane-bound ATPase activity was less sensitive to DCCD, averaging only 48% (76% of the wild type level). Six additional mutants, E21A, Q24A, E29A, R51A, K54A, and R93A had properties that were intermediate between the other two classes and were more similar to the wild type.Membranes from all mutants were subjected to immunoblotting using two previously described monoclonal antibodies to ∊(23Aggeler R. Mendel-Hartvig J. Capaldi R.A. Biochemistry. 1990; 29: 10387-10393Crossref PubMed Scopus (25) Google Scholar). Both monoclonal antibodies failed to recognize F16A, and S10A was recognized only weakly. The only significant difference between the two antibodies was that ∊I failed to recognize E21A and Q24A.A sequence alignment and secondary structure prediction was obtained using the EMBL PHD system(32Rost B. Sander C. J. Mol. Biol. 1993; 232: 584-599Crossref PubMed Scopus (2636) Google Scholar), and results of the secondary structure predictions are summarized in Fig. 2. Sequences from seven ∊ subunits were found to have greater than 30% identity with that of E. coli, and those were used in the secondary structure prediction. Six regions of β-strand were predicted in the first 78 amino acids, and three regions of α-helix were predicted at the carboxyl terminus(79-138), consisting of 5, 16, and 21 residues. Two other regions were weakly predicted to be β-strands: residues 15-16 and 32-34. Each of the 8 predicted β-strands shown in Fig. 2has 2 hydrophobic residues separated by a single residue. These residues are underlined. This trend exists in each of the eight ∊ sequences and is consistent with amphipathic β-strands, such as those which would form a β-barrel or β-sandwich. The only exception is the second predicted β-strand, in which the 2 hydrophobic residues are adjacent. Further analysis of 21 bacterial ∊ sequences retrieved by BLAST analysis (33Altschul S.F. Gish W. Miller W. Myers E.W. Lipman D.J. J. Mol. Biol. 1990; 215: 403-410Crossref PubMed Scopus (69041) Google Scholar) revealed that this pattern of hydrophobic residues was present in each sequence (not shown).Figure 2:PHD prediction of secondary structure of ∊. The lines labeled AA contain the amino acid sequence of ∊, with the corresponding numbers above. PHD indicates the profile network prediction, where E indicates β-strand, H indicates α-helix, and blank spaces indicate a loop prediction. Rel indicates the reliability index of prediction, on a scale from 0 to 9 (most reliable). SUB indicates a subset of the predictions for which the expected accuracy will be >82%. In this line, loop predictions are indicated by L. Where Rel < 5, no prediction is made, and these are indicated by dots. Underlined residues are the hydrophobic residues in the predicted β-strands. Other sequences used in the prediction are Vibrio alginolyticus, Bacillus PS3, Bacillus megaterium, Bacillus firmus, Rhodospirillum rubrum, Synechocystis PCC 6803, and Synechococcus PCC 6301.View Large Image Figure ViewerDownload Hi-res image Download (PPT)DISCUSSIONThe ∊ subunit has a central role in energy transduction by the E. coli F1-F0 ATP synthase. The goal of the present work was to identify by mutagenesis individual amino acids in ∊ that are involved in specific roles. Since amino acid replacements can cause a variety of nonlocal effects on protein structure, alanine-scanning mutagenesis (22Wells J.A. Methods Enzymol. 1991; 202: 390-411Crossref PubMed Scopus (309) Google Scholar) was used in this study.Two mutants, S10A and F16A, seemed to be defective in assembly of the ATP synthase. Residue 10 is predicted to be in the first turn following the first β-strand. The mutant S10C has previously been characterized (13Aggeler R. Chicas-Cruz K. Lai S.X. Keana J.F.W. Capaldi R.A. Biochemistry. 1992; 31: 2956-2961Crossref PubMed Scopus (95) Google Scholar) and has been cross-linked to γ via the thiol group. Our results are consistent with the location of residue 10 being near the ∊-γ interface. In addition, introduction of alanine at position 10 creates a hydrophobic patch at residues 8-11 (VVAA) that may promote misfolding or ∊-∊ aggregation. Others (18Skakoon E.N. Dunn S.D. Arch. Biochem. Biophys. 1993; 302: 279-284Crossref PubMed Scopus (14) Google Scholar) have observed that a deletion of residues 7-14 in ∊ caused aggregation.The mutant F16A is even less functional. Residue 16 resides in the second predicted β-strand at a position that is always Phe, Tyr, or Trp. Other studies have shown that amino-terminal deletions in ∊ of 16 residues are much more severe than are deletions of only 15 residues (17Jounouchi M. Takeyama M. Noumi T. Moriyama Y. Maeda M. Futai M. Arch. Biochem. Biophys. 1992; 292: 87-94Crossref PubMed Scopus (28) Google Scholar). Our results also indicate a critical role for residue 16.Residues Thr-77, Asp-81, Thr-82 and Arg-85 seem to be important for the binding of ∊ to other subunits. Each of the four mutants showed reduced levels of membrane-bound F1 ATPase activity, but this activity was not significantly inhibited by ∊ as shown by the low stimulation by LDAO (approximately 1.5 versus 3.1 for wild type). It is not unexpected that mutations that alter ∊ binding to other subunits, presumably γ(11Dunn S.D. J. Biol. Chem. 1982; 257: 7354-7359Abstract Full Text PDF PubMed Google Scholar), would reduce both the binding of F1 to F0 in membranes and also the inhibition of ATPase activity by ∊. These residues extend from the eighth predicted β-strand to the α-helical region. This region has been implicated previously in the inhibitory role of ∊ by analysis of deletions from the carboxyl terminus(16Kuki M. Noumi T. Maeda M. Amemura A. Futai M. J. Biol. Chem. 1988; 263: 17437-17442Abstract Full Text PDF PubMed Google Scholar). It has also been shown that an ∊-monoclonal antibody has an epitope that is buried in F1 and part of that epitope is between residues 78 and 85 (18Skakoon E.N. Dunn S.D. Arch. Biochem. Biophys. 1993; 302: 279-284Crossref PubMed Scopus (14) Google Scholar). Two other mutants analyzed in this study have similar properties: T43A and K46A. These residues lie in a stretch of 15 amino acids between the fourth and fifth predicted β-strands. It is possible that these residues make up part of the epitope for ∊-mAb-1, described previously(18Skakoon E.N. Dunn S.D. Arch. Biochem. Biophys. 1993; 302: 279-284Crossref PubMed Scopus (14) Google Scholar).A second group of mutants has an opposite effect on the inhibitory properties of ∊. This group also contains residues from two distinct regions: residue 31 and residues 59-70. With these mutants a relatively high level of F1 is bound to membranes, averaging 85% of wild type, but the ATPase activity is highly inhibited. Upon addition of LDAO, the activity is stimulated up to 4.6-fold. It appears that these mutants, E31A, E59A, E60A/K54A, E59A/E60A, S65A, and E70A, form stable F1-F0 complexes, but that the F1 is locked in an abnormally inhibited state.Residue Glu-31 is located in the fourth predicted β-strand and has been mutagenized previously(27LaRoe D.L. Vik S.B. J. Bacteriol. 1992; 174: 633-637Crossref PubMed Google Scholar). This residue has also been identified as the site of partial suppressors of the c subunit mutant Q42E(34Zhang Y. Oldenburg M. Fillingame R.H. J. Biol. Chem. 1994; 269: 10221-10224Abstract Full Text PDF PubMed Google Scholar). In the present study, E31A was found to reduce the sensitivity of F1 ATPase to DCCD (76% of wild type). Mutations at the nearby residue His-38 were shown (27LaRoe D.L. Vik S.B. J. Bacteriol. 1992; 174: 633-637Crossref PubMed Google Scholar) to reduce the sensitivity of F1 ATPase to DCCD. This evidence tends to support either of the possibilities offered(34Zhang Y. Oldenburg M. Fillingame R.H. J. Biol. Chem. 1994; 269: 10221-10224Abstract Full Text PDF PubMed Google Scholar), that this region of ∊ interacts directly with the polar loop of c or that it interacts with “stalk” subunits, which interact with c. A reduced sensitivity to DCCD by membrane-bound F1 ATPase is also seen with most of the other members of this group, especially S65A and E70A (70% and 62% of wild type, respectively). Residue 65 is predicted to lie in a loop between the sixth and seventh β-strands, and residue 70 is part of the predicted seventh β-strand. The double mutant E59A/E60A also has reduced sensitivity to DCCD, and these residues are found in a loop between the fifth and sixth predicted β-strands.The two monoclonal antibodies have been characterized previously with respect to binding of ∊ in F1 and F1-F0, ∊I (5A3-C11), and ∊II (13A7-E9) (23Aggeler R. Mendel-Hartvig J. Capaldi R.A. Biochemistry. 1990; 29: 10387-10393Crossref PubMed Scopus (25) Google Scholar). Our results reveal a single difference between these two antibodies: ∊I does not recognize the mutants E21A and Q24A. Since it was determined previously (23Aggeler R. Mendel-Hartvig J. Capaldi R.A. Biochemistry. 1990; 29: 10387-10393Crossref PubMed Scopus (25) Google Scholar) that ∊I does not recognize ∊ in F1-F0 complexes, but that ∊II does weakly, it is likely that residues 21 and 24 of ∊ are shielded by the binding of F1 to F0.The results of the present study contribute to the growing picture of the role of ∊ in the structure and function of the F1-F0 ATP synthase. Previous work by others has indicated a two-domain structure for ∊(16Kuki M. Noumi T. Maeda M. Amemura A. Futai M. J. Biol. Chem. 1988; 263: 17437-17442Abstract Full Text PDF PubMed Google Scholar), separated by a “hinge” region near residues 80-85(18Skakoon E.N. Dunn S.D. Arch. Biochem. Biophys. 1993; 302: 279-284Crossref PubMed Scopus (14) Google Scholar). Conformational changes in ∊ have been observed via differences in protease sensitivity and in response to ligands at nucleotide binding sites(19Mendel-Hartvig J. Capaldi R.A. Biochemistry. 1991; 30: 1278-1284Crossref PubMed Scopus (71) Google Scholar). DCCD has been shown to alter the nucleotide dependence of trypsin cleavage(35Mendel-Hartvig J. Capaldi R.A. Biochemistry. 1991; 30: 10987-10991Crossref PubMed Scopus (55) Google Scholar). Movement of ∊ relative to other F1 subunits has been observed via specific cross-linking reagents, also in response to ligands at nucleotide binding sites(6Gogol E.P. Johnston E. Aggeler R. Capaldi R.A. Proc. Natl. Acad. Sci. U. S. A. 1990; 87: 9585-9589Crossref PubMed Scopus (100) Google Scholar, 12Dallmann H.G. Flynn T.G. Dunn S.D. J. Biol. Chem. 1992; 267: 18953-18960Abstract Full Text PDF PubMed Google Scholar, 13Aggeler R. Chicas-Cruz K. Lai S.X. Keana J.F.W. Capaldi R.A. Biochemistry. 1992; 31: 2956-2961Crossref PubMed Scopus (95) Google Scholar). The effects of several mutations at the strictly conserved residue His-38 have also been studied. H38C has been shown to be accessible to Ellman's reagent, and the modified ∊ remains inhibitory(36Skakoon E.N. Dunn S.D. Arch. Biochem. Biophys. 1993; 302: 272-278Crossref PubMed Scopus (30) Google Scholar). Two other mutants H38I and H38R were found to have decreased sensitivity to DCCD, but ∊ seemed to be highly inhibitory(27LaRoe D.L. Vik S.B. J. Bacteriol. 1992; 174: 633-637Crossref PubMed Google Scholar).A schematic model for the secondary structure of ∊ is shown in Fig. 3, based on the PHD secondary structure predictions, hydrophobicity considerations, and the results of our mutagenesis. The model is oriented such that the top would be near the α and β subunits in F1, and the bottom would be near the c subunits of F0. The model includes 8 antiparallel β-strands, 3 α-helical segments, and one significant region of undesignated secondary structure." @default.
• W2106345114 created "2016-06-24" @default.
• W2106345114 creator A5051977441 @default.
• W2106345114 creator A5069609913 @default.
• W2106345114 date "1995-10-01" @default.
• W2106345114 modified "2023-09-27" @default.
• W2106345114 title "Alanine-scanning Mutagenesis of the ∊ Subunit of the F1-F0 ATP Synthase from Escherichia coli Reveals Two Classes of Mutants" @default.
• W2106345114 cites W145875931 @default.
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