FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106355752> ?p ?o ?g. }

• W2106355752 endingPage "9315" @default.
• W2106355752 startingPage "9306" @default.
• W2106355752 abstract "In many bacteria and archaea, an ancestral pathway is used where asparagine and glutamine are formed from their acidic precursors while covalently linked to tRNA(Asn) and tRNA(Gln), respectively. Stable complexes formed by the enzymes of these indirect tRNA aminoacylation pathways are found in several thermophilic organisms, and are called transamidosomes. We describe here a transamidosome forming Gln-tRNA(Gln) in Helicobacter pylori, an ε-proteobacterium pathogenic for humans; this transamidosome displays novel properties that may be characteristic of mesophilic organisms. This ternary complex containing the non-canonical GluRS2 specific for Glu-tRNA(Gln) formation, the tRNA-dependent amidotransferase GatCAB and tRNA(Gln) was characterized by dynamic light scattering. Moreover, we observed by interferometry a weak interaction between GluRS2 and GatCAB (K(D) = 40 ± 5 µM). The kinetics of Glu-tRNA(Gln) and Gln-tRNA(Gln) formation indicate that conformational shifts inside the transamidosome allow the tRNA(Gln) acceptor stem to interact alternately with GluRS2 and GatCAB despite their common identity elements. The integrity of this dynamic transamidosome depends on a critical concentration of tRNA(Gln), above which it dissociates into separate GatCAB/tRNA(Gln) and GluRS2/tRNA(Gln) complexes. Ester bond protection assays show that both enzymes display a good affinity for tRNA(Gln) regardless of its aminoacylation state, and support a mechanism where GluRS2 can hydrolyze excess Glu-tRNA(Gln), ensuring faithful decoding of Gln codons." @default.
• W2106355752 created "2016-06-24" @default.
• W2106355752 creator A5004096613 @default.
• W2106355752 creator A5006682222 @default.
• W2106355752 creator A5007226771 @default.
• W2106355752 creator A5008649323 @default.
• W2106355752 creator A5061894291 @default.
• W2106355752 creator A5063419170 @default.
• W2106355752 creator A5068518155 @default.
• W2106355752 creator A5082328497 @default.
• W2106355752 creator A5086636135 @default.
• W2106355752 date "2011-08-03" @default.
• W2106355752 modified "2023-10-18" @default.
• W2106355752 title "Gln-tRNAGln synthesis in a dynamic transamidosome from Helicobacter pylori, where GluRS2 hydrolyzes excess Glu-tRNAGln" @default.
• W2106355752 cites W1000298608 @default.
• W2106355752 cites W1565590096 @default.
• W2106355752 cites W1575542444 @default.
• W2106355752 cites W1658189890 @default.
• W2106355752 cites W1774953805 @default.
• W2106355752 cites W1967448903 @default.
• W2106355752 cites W1973363547 @default.
• W2106355752 cites W1977219542 @default.
• W2106355752 cites W1983867616 @default.
• W2106355752 cites W1993111592 @default.
• W2106355752 cites W1996870953 @default.
• W2106355752 cites W1997638590 @default.
• W2106355752 cites W2002895409 @default.
• W2106355752 cites W2005747480 @default.
• W2106355752 cites W2011628818 @default.
• W2106355752 cites W2016085798 @default.
• W2106355752 cites W2017102688 @default.
• W2106355752 cites W2018645572 @default.
• W2106355752 cites W2026350792 @default.
• W2106355752 cites W2037825700 @default.
• W2106355752 cites W2043100866 @default.
• W2106355752 cites W2048432086 @default.
• W2106355752 cites W2050266415 @default.
• W2106355752 cites W2068652047 @default.
• W2106355752 cites W2073265639 @default.
• W2106355752 cites W2082487774 @default.
• W2106355752 cites W2085118852 @default.
• W2106355752 cites W2106687404 @default.
• W2106355752 cites W2119662144 @default.
• W2106355752 cites W2136201971 @default.
• W2106355752 cites W2140020971 @default.
• W2106355752 cites W2147072283 @default.
• W2106355752 cites W2147302373 @default.
• W2106355752 cites W2147488317 @default.
• W2106355752 cites W2153693600 @default.
• W2106355752 doi "https://doi.org/10.1093/nar/gkr619" @default.
• W2106355752 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3241645" @default.
• W2106355752 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21813455" @default.
• W2106355752 hasPublicationYear "2011" @default.
• W2106355752 type Work @default.
• W2106355752 sameAs 2106355752 @default.
• W2106355752 citedByCount "26" @default.
• W2106355752 countsByYear W21063557522012 @default.
• W2106355752 countsByYear W21063557522013 @default.
• W2106355752 countsByYear W21063557522014 @default.
• W2106355752 countsByYear W21063557522015 @default.
• W2106355752 countsByYear W21063557522016 @default.
• W2106355752 countsByYear W21063557522017 @default.
• W2106355752 countsByYear W21063557522019 @default.
• W2106355752 countsByYear W21063557522020 @default.
• W2106355752 countsByYear W21063557522021 @default.
• W2106355752 crossrefType "journal-article" @default.
• W2106355752 hasAuthorship W2106355752A5004096613 @default.
• W2106355752 hasAuthorship W2106355752A5006682222 @default.
• W2106355752 hasAuthorship W2106355752A5007226771 @default.
• W2106355752 hasAuthorship W2106355752A5008649323 @default.
• W2106355752 hasAuthorship W2106355752A5061894291 @default.
• W2106355752 hasAuthorship W2106355752A5063419170 @default.
• W2106355752 hasAuthorship W2106355752A5068518155 @default.
• W2106355752 hasAuthorship W2106355752A5082328497 @default.
• W2106355752 hasAuthorship W2106355752A5086636135 @default.
• W2106355752 hasBestOaLocation W21063557521 @default.
• W2106355752 hasConcept C104317684 @default.
• W2106355752 hasConcept C151238385 @default.
• W2106355752 hasConcept C153957851 @default.
• W2106355752 hasConcept C21312847 @default.
• W2106355752 hasConcept C2775895851 @default.
• W2106355752 hasConcept C2779349466 @default.
• W2106355752 hasConcept C515207424 @default.
• W2106355752 hasConcept C55493867 @default.
• W2106355752 hasConcept C67705224 @default.
• W2106355752 hasConcept C75972863 @default.
• W2106355752 hasConcept C86803240 @default.
• W2106355752 hasConceptScore W2106355752C104317684 @default.
• W2106355752 hasConceptScore W2106355752C151238385 @default.
• W2106355752 hasConceptScore W2106355752C153957851 @default.
• W2106355752 hasConceptScore W2106355752C21312847 @default.
• W2106355752 hasConceptScore W2106355752C2775895851 @default.
• W2106355752 hasConceptScore W2106355752C2779349466 @default.
• W2106355752 hasConceptScore W2106355752C515207424 @default.
• W2106355752 hasConceptScore W2106355752C55493867 @default.
• W2106355752 hasConceptScore W2106355752C67705224 @default.
• W2106355752 hasConceptScore W2106355752C75972863 @default.
• W2106355752 hasConceptScore W2106355752C86803240 @default.

• next