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- W2106366838 abstract "Background: Resistance among anaerobic bacteria is increasing and posing a major problem for clinical periodontology, maxillo-facial and cardiovascular surgery. In the present study, we attempted to determine susceptibility of clinical strains of anaerobic bacteria to fluoroquinolones (FQ) and the frequency for FQ-resistant isolates and to study some mechanisms of resistance. Methods: We evaluated 408 strains of anaerobic (Actinomyces israelii, Bacteroides forsithus, Fusobacterium nucleatum, Peptostreptococcus anaerobius, Porphyromonas gingivalis, Prevotella intermedia, Streptococcus intermedius) and microaerophilic bacteria (H. actinomycetemcomitans, A. naeslundii, S. milleri) from different patients with oral diseases, which were collected and tested. The cultivation was carried out in an anaerobic jar with gas mixture (80% N2; 10% CO2; 10% H2). MICs were determined in vitro for Gemi-, Gati-, Moxi-, Levo-, Spar- and Ciprofloxacin against these bacteria according to NCCLS, using dilution method. Besides, then we have examined the chromosomal mutations in the FQ-resistance-determining region of gyrA, gyrB genes and parC, parE. Results: Establishment of the variability of anaerobic bacteria susceptibility to FQ of various generations. High level of susceptibility to the generations III-IV FQ was demonstrated for the tested strains. Gemifloxacin was the most active and potent regarding all strains of anaerobic bacteria (susceptibility 100%, MIC90 0,012–0,05 mg/L). In comparison: Moxi - 98,5%, MIC90 0,025–0,25 mg/L, Gati - 86,1%, MIC90 0,05–0,5 mg/L (generation IV), spar - 85%, MIC90 0,25–0,5 mg/L, Levo - 85%, MIC90 0,25–1 mg/L (generation III), and Ciprofloxacin - 70%, MIC90 1–2,5 mg/L (generation II). In FQ-resistant isolates we determined nucleotide sequences of the FQ-resistant regions of gyrA, gyrB genes and parC, parE. In 18 clinical FQ-resistant isolates (S.intermedius and P. gingivalis) we have revealed mutations in the FQ-resistance-determining region of gyrA and parC genes - substitutions of serine 83 and 85 to Leucin. Clinical FQ-resistant isolates showed a wide variation in MIC, ranging from 2,5 to 12,5 mg/L. Conclusion: Generation IV FQ were found more effective. Among the group Gemifloxacin demonstrated excellent activity regarding all anaerobic bacteria. The most common mechanism of oral anaerobic bacteria resistance to FQ is the modification of type II (DNA gyrase) and IV topoisomerase. However, the difference in the level of resistance could not be explained only on the basis of mutations in gyrB or parE." @default.
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- W2106366838 date "2008-12-01" @default.
- W2106366838 modified "2023-09-26" @default.
- W2106366838 title "Susceptibility of Oral Anaerobic Bacteria to Fluoroquinolones of Various Generations and Molecular Characterization of Resistant Strains" @default.
- W2106366838 doi "https://doi.org/10.1016/j.ijid.2008.05.1052" @default.
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