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- W2106405081 endingPage "2459" @default.
- W2106405081 startingPage "2441" @default.
- W2106405081 abstract "Gas3/PMP22 plays a crucial role in regulating myelin formation and maintenance, and different genetic alterations in gas3/PMP22 are responsible for a set of human peripheral neuropathies. We have previously demonstrated that Gas3/PMP22 could regulate susceptibility to apoptosis in NIH3T3 cells but not in REF 52 cells. In this report we demonstrate that when the apoptotic response triggered by gas3/PMP22 was counteracted by Bcl-2 coexpression, morphological changes were observed. Time-lapse analysis confirmed that Gas3/PMP22 can modulate cell spreading, and this effect was strengthened after inhibition of phosphoinositide 3-kinase. Using the active form of the small GTPase RhoA, we have been able to dissect the different Gas3/PMP22 biological activities. RhoA counteracted the Gas3/PMP22-dependent morphological response but was unable to neutralize the apoptotic response. Treatment of NIH3T3 cells with cytotoxic necrotizing factor 1, which activates endogenous Rho, also counteracted Gas3/PMP22-mediated cell shape and spreading changes. Treatment of REF 52 cells, which are unresponsive to Gas3/PMP22 overexpression, with the C3 exoenzyme, inhibiting Rho activity, renders REF 52 cells responsive to Gas3/PMP22 overexpression for cell shape and spreading changes. Finally, assembly of stress fibers and focal adhesions complexes, in response to lysophosphatidic acid-induced endogenous Rho activation, was impaired in Gas3/PMP22-overexpressing cells. We hypothesize that cell shape and spreading regulated by Gas3/PMP22 through the Rho GTPase might have an important role during Schwann cells differentiation and myelinization." @default.
- W2106405081 created "2016-06-24" @default.
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- W2106405081 date "1999-07-01" @default.
- W2106405081 modified "2023-09-24" @default.
- W2106405081 title "Rho-dependent Regulation of Cell Spreading by the Tetraspan Membrane Protein Gas3/PMP22" @default.
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- W2106405081 doi "https://doi.org/10.1091/mbc.10.7.2441" @default.
- W2106405081 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/25466" @default.
- W2106405081 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10397775" @default.
- W2106405081 hasPublicationYear "1999" @default.
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