Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106455172> ?p ?o ?g. }
- W2106455172 abstract "Background: Hepatitis B virus (HBV) infection is a public health problem in developing countries. HBV genotypes play major role in the evolution of infection since they were involved in different clinical presentations and response to treatment. Objectives: This study was conducted to determine circulating HBV genotypes in the country and to evaluate the efficiency of restriction fragment length polymorphism (RFLP) analysis for HBV genotyping. Patients and Methods: We investigated 98 samples collected from patients chronically infected with HBV. HBV genotypes were determined by analysis of patterns obtained after amplification in Pre-S region and digestion of the amplicon by two endonucleases AvaII and DpnII. Obtained results were confirmed by partial sequencing in the same region. Results: Two different HBV genotypes were detected in this study, Genotype D (in 95. 9%) and Genotype A (in 4.1%). Seventy-four samples (75.5%) were successfully genotyped with RFLP analysis and all classified as genotype D. The remaining 24 samples (24.5%) which were un-genotyped by RFLP analysis, were classified by partial sequencing of the pre-S region as HBV genotype D (20 samples, 20.4%) and genotype A (4 samples, 4.1%). Atypical profiles were significantly associated with advanced liver disease (P = 0.001) as well as older age (P < 0.05). Conclusions: Several previous studies used PCR-RFLP to genotype HBV; however, we showed the high risk to obtain atypical profiles, especially in advanced stages of chronic infection, with as results difficulties to genotype the virus. These profiles resulted from the accumulation of mutations during natural course of infection resulting in a modification in restriction sites for enzymes. So, we recommended completing the investigation by partial sequencing to confirm obtained results." @default.
- W2106455172 created "2016-06-24" @default.
- W2106455172 creator A5002310931 @default.
- W2106455172 creator A5026212286 @default.
- W2106455172 creator A5029805211 @default.
- W2106455172 creator A5044532548 @default.
- W2106455172 creator A5075489748 @default.
- W2106455172 creator A5078947210 @default.
- W2106455172 creator A5085674500 @default.
- W2106455172 date "2013-10-15" @default.
- W2106455172 modified "2023-09-26" @default.
- W2106455172 title "Evaluation of PCR-RFLP in the Pre-S Region as Molecular Method for Hepatitis B Virus Genotyping" @default.
- W2106455172 cites W1597477033 @default.
- W2106455172 cites W1606019832 @default.
- W2106455172 cites W1969818818 @default.
- W2106455172 cites W1971248139 @default.
- W2106455172 cites W1972620184 @default.
- W2106455172 cites W1973678305 @default.
- W2106455172 cites W1974381921 @default.
- W2106455172 cites W1979190243 @default.
- W2106455172 cites W1979438148 @default.
- W2106455172 cites W1981456217 @default.
- W2106455172 cites W1982184546 @default.
- W2106455172 cites W1984513955 @default.
- W2106455172 cites W1985071920 @default.
- W2106455172 cites W2000336618 @default.
- W2106455172 cites W2010680280 @default.
- W2106455172 cites W2018115348 @default.
- W2106455172 cites W2020818462 @default.
- W2106455172 cites W2027592155 @default.
- W2106455172 cites W2039709676 @default.
- W2106455172 cites W2039957427 @default.
- W2106455172 cites W2047733064 @default.
- W2106455172 cites W2049298824 @default.
- W2106455172 cites W2052036402 @default.
- W2106455172 cites W2054764460 @default.
- W2106455172 cites W2061364024 @default.
- W2106455172 cites W2064589208 @default.
- W2106455172 cites W2064695462 @default.
- W2106455172 cites W2069869637 @default.
- W2106455172 cites W2077040241 @default.
- W2106455172 cites W2081556170 @default.
- W2106455172 cites W2081603190 @default.
- W2106455172 cites W2083651334 @default.
- W2106455172 cites W2092973452 @default.
- W2106455172 cites W2100916717 @default.
- W2106455172 cites W2108308364 @default.
- W2106455172 cites W2116369632 @default.
- W2106455172 cites W2133129754 @default.
- W2106455172 cites W2135201594 @default.
- W2106455172 cites W2136445954 @default.
- W2106455172 cites W2142834280 @default.
- W2106455172 cites W2144375124 @default.
- W2106455172 cites W2145620204 @default.
- W2106455172 cites W2145916511 @default.
- W2106455172 cites W2166274558 @default.
- W2106455172 cites W1996535930 @default.
- W2106455172 doi "https://doi.org/10.5812/hepatmon.11781" @default.
- W2106455172 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3842526" @default.
- W2106455172 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24348634" @default.
- W2106455172 hasPublicationYear "2013" @default.
- W2106455172 type Work @default.
- W2106455172 sameAs 2106455172 @default.
- W2106455172 citedByCount "6" @default.
- W2106455172 countsByYear W21064551722015 @default.
- W2106455172 countsByYear W21064551722016 @default.
- W2106455172 countsByYear W21064551722017 @default.
- W2106455172 countsByYear W21064551722018 @default.
- W2106455172 countsByYear W21064551722022 @default.
- W2106455172 crossrefType "journal-article" @default.
- W2106455172 hasAuthorship W2106455172A5002310931 @default.
- W2106455172 hasAuthorship W2106455172A5026212286 @default.
- W2106455172 hasAuthorship W2106455172A5029805211 @default.
- W2106455172 hasAuthorship W2106455172A5044532548 @default.
- W2106455172 hasAuthorship W2106455172A5075489748 @default.
- W2106455172 hasAuthorship W2106455172A5078947210 @default.
- W2106455172 hasAuthorship W2106455172A5085674500 @default.
- W2106455172 hasBestOaLocation W21064551721 @default.
- W2106455172 hasConcept C104317684 @default.
- W2106455172 hasConcept C128319531 @default.
- W2106455172 hasConcept C135763542 @default.
- W2106455172 hasConcept C159047783 @default.
- W2106455172 hasConcept C168007829 @default.
- W2106455172 hasConcept C2522874641 @default.
- W2106455172 hasConcept C2780593183 @default.
- W2106455172 hasConcept C31467283 @default.
- W2106455172 hasConcept C49105822 @default.
- W2106455172 hasConcept C54355233 @default.
- W2106455172 hasConcept C71924100 @default.
- W2106455172 hasConcept C8185291 @default.
- W2106455172 hasConcept C86803240 @default.
- W2106455172 hasConceptScore W2106455172C104317684 @default.
- W2106455172 hasConceptScore W2106455172C128319531 @default.
- W2106455172 hasConceptScore W2106455172C135763542 @default.
- W2106455172 hasConceptScore W2106455172C159047783 @default.
- W2106455172 hasConceptScore W2106455172C168007829 @default.
- W2106455172 hasConceptScore W2106455172C2522874641 @default.
- W2106455172 hasConceptScore W2106455172C2780593183 @default.
- W2106455172 hasConceptScore W2106455172C31467283 @default.
- W2106455172 hasConceptScore W2106455172C49105822 @default.