FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106470598> ?p ?o ?g. }

• W2106470598 endingPage "57" @default.
• W2106470598 startingPage "52" @default.
• W2106470598 abstract "Ureaplasma urealyticum comprises 14 serotypes. The existing serotyping methods all use polyclonal antibodies. These methods are time-consuming and labor-intensive, and they cannot always be performed on primary isolates; in addition, the results are difficult to interpret. We developed a new enzyme-linked immunosorbent assay (ELISA) method using serotype-specific monoclonal antibodies (MAbs) to enable the serotyping of U. urealyticum isolates from primary broth cultures. Each of the 14 serotype reference strains was tested against 14 selected MAbs. Homologous reactions were very strong, while heterologous reactions were negligible. Three cross-reactions were observed: MAb 5 cross-reacted with serotype 2, MAb 14 cross-reacted with serotype 3, and MAb 8 cross-reacted with serotype 13. Despite the cross-reactions observed, all the serotype reference strains of U. urealyticum could be identified and differentiated using a combination of MAbs. Reproducibility was analyzed with a fractionated antigenic preparation and with several freshly prepared antigens of the same strain. No significant interrun variation was found with the fractionated antigen, but significant variations in optical density (OD) values were found when freshly prepared antigens were tested. However, the variation in OD values did not influence the overall interpretation of the ELISA: reactions with homologous MAbs were always prominent compared to those of the negative controls. This newly developed ELISA using MAbs seems promising for serotyping of U. urealyticum clinical isolates." @default.
• W2106470598 created "2016-06-24" @default.
• W2106470598 creator A5009171869 @default.
• W2106470598 creator A5014534403 @default.
• W2106470598 creator A5036014918 @default.
• W2106470598 creator A5063743099 @default.
• W2106470598 date "2001-01-01" @default.
• W2106470598 modified "2023-10-12" @default.
• W2106470598 title "Development of an Enzyme-Linked Immunosorbent Assay for Serotyping <i>Ureaplasma urealyticum</i> Strains Using Monoclonal Antibodies" @default.
• W2106470598 cites W1509277618 @default.
• W2106470598 cites W1540763980 @default.
• W2106470598 cites W1551499932 @default.
• W2106470598 cites W1599399841 @default.
• W2106470598 cites W1632795955 @default.
• W2106470598 cites W1683430312 @default.
• W2106470598 cites W1882128847 @default.
• W2106470598 cites W1942144208 @default.
• W2106470598 cites W1946946982 @default.
• W2106470598 cites W199908055 @default.
• W2106470598 cites W2004781440 @default.
• W2106470598 cites W2009020300 @default.
• W2106470598 cites W2016641643 @default.
• W2106470598 cites W2026192798 @default.
• W2106470598 cites W2032258057 @default.
• W2106470598 cites W2041385092 @default.
• W2106470598 cites W2043573892 @default.
• W2106470598 cites W2054112599 @default.
• W2106470598 cites W2088780315 @default.
• W2106470598 cites W2109164892 @default.
• W2106470598 cites W2113680301 @default.
• W2106470598 cites W2129769027 @default.
• W2106470598 cites W2158472106 @default.
• W2106470598 cites W2159752086 @default.
• W2106470598 cites W2161420885 @default.
• W2106470598 cites W2167482017 @default.
• W2106470598 cites W2169516102 @default.
• W2106470598 cites W2171973844 @default.
• W2106470598 cites W2191917379 @default.
• W2106470598 cites W2411626092 @default.
• W2106470598 doi "https://doi.org/10.1128/cdli.8.1.52-57.2001" @default.
• W2106470598 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/96010" @default.
• W2106470598 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11139195" @default.
• W2106470598 hasPublicationYear "2001" @default.
• W2106470598 type Work @default.
• W2106470598 sameAs 2106470598 @default.
• W2106470598 citedByCount "13" @default.
• W2106470598 countsByYear W21064705982013 @default.
• W2106470598 countsByYear W21064705982015 @default.
• W2106470598 countsByYear W21064705982017 @default.
• W2106470598 countsByYear W21064705982018 @default.
• W2106470598 countsByYear W21064705982019 @default.
• W2106470598 crossrefType "journal-article" @default.
• W2106470598 hasAuthorship W2106470598A5009171869 @default.
• W2106470598 hasAuthorship W2106470598A5014534403 @default.
• W2106470598 hasAuthorship W2106470598A5036014918 @default.
• W2106470598 hasAuthorship W2106470598A5063743099 @default.
• W2106470598 hasBestOaLocation W21064705981 @default.
• W2106470598 hasConcept C10389963 @default.
• W2106470598 hasConcept C104317684 @default.
• W2106470598 hasConcept C147483822 @default.
• W2106470598 hasConcept C159047783 @default.
• W2106470598 hasConcept C159654299 @default.
• W2106470598 hasConcept C203014093 @default.
• W2106470598 hasConcept C2776672898 @default.
• W2106470598 hasConcept C2778838687 @default.
• W2106470598 hasConcept C2780115692 @default.
• W2106470598 hasConcept C2781209916 @default.
• W2106470598 hasConcept C542903549 @default.
• W2106470598 hasConcept C55493867 @default.
• W2106470598 hasConcept C86803240 @default.
• W2106470598 hasConcept C89423630 @default.
• W2106470598 hasConcept C956191 @default.
• W2106470598 hasConceptScore W2106470598C10389963 @default.
• W2106470598 hasConceptScore W2106470598C104317684 @default.
• W2106470598 hasConceptScore W2106470598C147483822 @default.
• W2106470598 hasConceptScore W2106470598C159047783 @default.
• W2106470598 hasConceptScore W2106470598C159654299 @default.
• W2106470598 hasConceptScore W2106470598C203014093 @default.
• W2106470598 hasConceptScore W2106470598C2776672898 @default.
• W2106470598 hasConceptScore W2106470598C2778838687 @default.
• W2106470598 hasConceptScore W2106470598C2780115692 @default.
• W2106470598 hasConceptScore W2106470598C2781209916 @default.
• W2106470598 hasConceptScore W2106470598C542903549 @default.
• W2106470598 hasConceptScore W2106470598C55493867 @default.
• W2106470598 hasConceptScore W2106470598C86803240 @default.
• W2106470598 hasConceptScore W2106470598C89423630 @default.
• W2106470598 hasConceptScore W2106470598C956191 @default.
• W2106470598 hasIssue "1" @default.
• W2106470598 hasLocation W21064705981 @default.
• W2106470598 hasLocation W21064705982 @default.
• W2106470598 hasLocation W21064705983 @default.
• W2106470598 hasLocation W21064705984 @default.
• W2106470598 hasOpenAccess W2106470598 @default.
• W2106470598 hasPrimaryLocation W21064705981 @default.
• W2106470598 hasRelatedWork W1942144208 @default.
• W2106470598 hasRelatedWork W1982126292 @default.
• W2106470598 hasRelatedWork W2006959176 @default.
• W2106470598 hasRelatedWork W2161420885 @default.

• next