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• W2106485617 abstract "We utilized a mammalian expression system to purify and characterize autotaxin (ATX)/lysophospholipase D, an enzyme present in the blood responsible for biosynthesis of lysophosphatidic acid. The human ATX cDNA encoding amino acids 29-915 was cloned downstream of a secretion signal of CD5. At the carboxyl terminus was a thrombin cleavage site followed by the constant domain (Fc) of IgG to facilitate protein purification. The ATX-Fc fusion protein was expressed in HEK293 cells and isolated from conditioned medium of a stable clone by affinity chromatography with Protein A sepharose followed by cleavage with thrombin. The untagged ATX protein was further purified to essential homogeneity by gel filtration chromatography with a yield of approximately 5 mg/liter medium. The purified ATX protein was enzymatically active and biologically functional, offering a useful tool for further biological and structural studies of this important enzyme." @default.
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• W2106485617 date "2010-08-31" @default.
• W2106485617 modified "2023-09-26" @default.
• W2106485617 title "Large scale purification and characterization of recombinant human autotaxin/lysophospholipase D from mammalian cells" @default.
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• W2106485617 doi "https://doi.org/10.5483/bmbrep.2010.43.8.541" @default.
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