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- W2106530530 abstract "Cuticle ultrastructure and radiolabelling of isolated cuticles after incorporation of [ 14 C] acetate in foliar discs were investigated with ivy plants grown in vitro then ex vitro . Results show an increase in thickness, mass and wax content, between young and expanded leaves, for both in vitro and ex vitro cuticles. The cuticle of in vitro unexpanded leaves was very thin and only constituted a lamellate zone. The ultrastructure of in vitro young and expanded leaf cuticles showed characteristics similar to in situ cuticles. The thickness of the lamellate zone remained fairly constant and represented 33% of the cuticle thickness in young leaves, but only 11.4% in expanded leaves. The number of lamellar units decreased from 14 to nine between these two growth stages. The main difference between young leaves developed in vitro or ex vitro was a thinner lamellate zone for ex vitro cuticles. However, these latter cuticles had an intermediary zone between the lamellate and reticulate zones. The cuticle thickness of expanded leaves was greater for in vitro cuticles suggesting a temporary decrease in cuticle biosynthesis after transfer of the plant from in vitro to ex vitro. Results from cuticle radiolabelling show higher radioactivity incorporation in cuticles isolated from leaves developed ex vitro compared to in vitro . This radiolabelling was particularly marked for ex vitro young leaf cuticles and depended on the duration of the ex vitro growth period revealing a progressive activation of cuticle biosynthesis in response to new environmental conditions." @default.
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- W2106530530 date "1997-08-01" @default.
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- W2106530530 title "Ultrastructure and Radiolabelling of Leaf Cuticles from Ivy (Hedera helixL.) Plantsin vitroand duringex vitroAcclimatization" @default.
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- W2106530530 doi "https://doi.org/10.1006/anbo.1997.0414" @default.
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