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• W2106540627 abstract "Abstract Background Tra1 is an essential protein in Saccharomyces cerevisiae . It was first identified in the SAGA and NuA4 complexes, both with functions in multiple aspects of gene regulation and DNA repair, and recently found in the ASTRA complex. Tra1 belongs to the PIKK family of proteins with a C-terminal PI3K domain followed by a FATC domain. Previously we found that mutation of leucine to alanine at position 3733 in the FATC domain of Tra1 ( tra1-L3733A ) results in transcriptional changes and slow growth under conditions of stress. To further define the regulatory interactions of Tra1 we isolated extragenic suppressors of the tra1-L3733A allele. Results We screened for suppressors of the ethanol sensitivity caused by tra1-L3733A . Eleven extragenic recessive mutations, belonging to three complementation groups, were identified that partially suppressed a subset of the phenotypes caused by tra 1-L3733A . Using whole genome sequencing we identified one of the mutations as an opal mutation at tryptophan 165 of UPF1/NAM7 . Partial suppression of the transcriptional defect resulting from tra1-L3733A was observed at GAL10 , but not at PHO5 . Suppression was due to loss of nonsense mediated decay (NMD) since deletion of any one of the three NMD surveillance components ( upf1/nam7, upf2/nmd2 , or upf3 ) mediated the effect. Deletion of upf1 suppressed a second FATC domain mutation, tra1-F3744A , as well as a mutation to the PIK3 domain. In contrast, deletions of SAGA or NuA4 components were not suppressed. Conclusions We have demonstrated a genetic interaction between TRA1 and genes of the NMD pathway. The suppression is specific for mutations in TRA1 . Since NMD and Tra1 generally act reciprocally to control gene expression, and the FATC domain mutations do not directly affect NMD, we suggest that suppression occurs as the result of overlap and/or crosstalk in these two broad regulatory networks." @default.
• W2106540627 created "2016-06-24" @default.
• W2106540627 creator A5020938159 @default.
• W2106540627 creator A5027317840 @default.
• W2106540627 creator A5091395679 @default.
• W2106540627 date "2012-03-22" @default.
• W2106540627 modified "2023-10-09" @default.
• W2106540627 title "Loss of nonsense mediated decay suppresses mutations in Saccharomyces cerevisiae TRA1" @default.
• W2106540627 cites W1645033312 @default.
• W2106540627 cites W1821435151 @default.
• W2106540627 cites W1940178156 @default.
• W2106540627 cites W1967390330 @default.
• W2106540627 cites W1968029309 @default.
• W2106540627 cites W1971011130 @default.
• W2106540627 cites W1975117841 @default.
• W2106540627 cites W1977480629 @default.
• W2106540627 cites W1978754000 @default.
• W2106540627 cites W1980548970 @default.
• W2106540627 cites W1981177571 @default.
• W2106540627 cites W1984356380 @default.
• W2106540627 cites W1987230839 @default.
• W2106540627 cites W1987577420 @default.
• W2106540627 cites W1989230236 @default.
• W2106540627 cites W1989277636 @default.
• W2106540627 cites W1996183355 @default.
• W2106540627 cites W1996973347 @default.
• W2106540627 cites W2003987858 @default.
• W2106540627 cites W2005855998 @default.
• W2106540627 cites W2006254819 @default.
• W2106540627 cites W2012288774 @default.
• W2106540627 cites W2013729196 @default.
• W2106540627 cites W2015881243 @default.
• W2106540627 cites W2018507091 @default.
• W2106540627 cites W2020151423 @default.
• W2106540627 cites W2021729787 @default.
• W2106540627 cites W2023620387 @default.
• W2106540627 cites W2030302003 @default.
• W2106540627 cites W2032475954 @default.
• W2106540627 cites W2034542637 @default.
• W2106540627 cites W2037579319 @default.
• W2106540627 cites W2048916500 @default.
• W2106540627 cites W2049738765 @default.
• W2106540627 cites W2050721857 @default.
• W2106540627 cites W2059553154 @default.
• W2106540627 cites W2061806029 @default.
• W2106540627 cites W2072590456 @default.
• W2106540627 cites W2072607093 @default.
• W2106540627 cites W2075161820 @default.
• W2106540627 cites W2075943942 @default.
• W2106540627 cites W2079810511 @default.
• W2106540627 cites W2088772708 @default.
• W2106540627 cites W2088857928 @default.
• W2106540627 cites W2103126892 @default.
• W2106540627 cites W2103502154 @default.
• W2106540627 cites W2105696572 @default.
• W2106540627 cites W2106755835 @default.
• W2106540627 cites W2108234281 @default.
• W2106540627 cites W2111578933 @default.
• W2106540627 cites W2111888550 @default.
• W2106540627 cites W2113725224 @default.
• W2106540627 cites W2115700741 @default.
• W2106540627 cites W2116287534 @default.
• W2106540627 cites W2116841494 @default.
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• W2106540627 cites W2127073167 @default.
• W2106540627 cites W2132264109 @default.
• W2106540627 cites W2133043413 @default.
• W2106540627 cites W2133102806 @default.
• W2106540627 cites W2136942244 @default.
• W2106540627 cites W2142812553 @default.
• W2106540627 cites W2144924084 @default.
• W2106540627 cites W2145902670 @default.
• W2106540627 cites W2157441710 @default.
• W2106540627 cites W2158235544 @default.
• W2106540627 cites W2161138122 @default.
• W2106540627 cites W2164228312 @default.
• W2106540627 cites W2164746379 @default.
• W2106540627 cites W2168747921 @default.
• W2106540627 cites W2170763327 @default.
• W2106540627 cites W4241302198 @default.
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• W2106540627 doi "https://doi.org/10.1186/1471-2156-13-19" @default.
• W2106540627 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3364908" @default.
• W2106540627 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22439631" @default.
• W2106540627 hasPublicationYear "2012" @default.
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