FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106548891> ?p ?o ?g. }

• W2106548891 endingPage "57" @default.
• W2106548891 startingPage "48" @default.
• W2106548891 abstract "Pancreatic triglyceride lipase (PTL) and the homologous pancreatic lipase related protein 2 (PLRP2) provide a unique opportunity to understand the molecular mechanism of lipolysis. They differ in substrate specificity, sensitivity to bile salts, and colipase dependence despite their close amino acid and tertiary structure identity. One important structure, present in both lipases, is the lid which occupies different positions in the inactive and active forms of PTL. We investigated the role of the lid in lipase function by site-specific mutagenesis. By exchanging the lids between PTL and PLRP2, we created two chimeric lipases. Additionally, we made multiple substitution mutations in the PTL lid. PLRP2 with the PTL lid had kinetic properties similar to PLRP2. PTL with the PLRP2 lid was greatly impaired and had no activity at micellar bile salt concentrations even in the presence of colipase. Both chimeras showed interfacial activation suggesting that the closed lid position was maintained. A series of substitution mutations were made in positions Arg257 and Asp258. These mutations demonstrated the importance of these two residues to maintaining the normal activity, triglyceride acyl chain specificity, and colipase interaction of PTL. The preserved interfacial activation in the chimeras, the similar crystal structure of the two lids in the closed position, and the importance of Arg257 and Asp258 in mediating the open conformation of the lid argue that the position of the open lid influences the differences in activity against triglycerides, in sensitivity to bile salts, and in colipase dependence between PTL and PLRP2. —Yang, Y., and M. E. Lowe. The open lid mediates pancreatic lipase function." @default.
• W2106548891 created "2016-06-24" @default.
• W2106548891 creator A5033268514 @default.
• W2106548891 creator A5043775067 @default.
• W2106548891 date "2000-01-01" @default.
• W2106548891 modified "2023-10-13" @default.
• W2106548891 title "The open lid mediates pancreatic lipase function" @default.
• W2106548891 cites W1528115394 @default.
• W2106548891 cites W1539365560 @default.
• W2106548891 cites W1574276740 @default.
• W2106548891 cites W1587176444 @default.
• W2106548891 cites W1597623702 @default.
• W2106548891 cites W1859800025 @default.
• W2106548891 cites W1927538892 @default.
• W2106548891 cites W1969172212 @default.
• W2106548891 cites W1980129832 @default.
• W2106548891 cites W1981765089 @default.
• W2106548891 cites W2001892038 @default.
• W2106548891 cites W2003143812 @default.
• W2106548891 cites W2007697108 @default.
• W2106548891 cites W2012176370 @default.
• W2106548891 cites W2028523580 @default.
• W2106548891 cites W2036414218 @default.
• W2106548891 cites W2046429206 @default.
• W2106548891 cites W2049506201 @default.
• W2106548891 cites W2054038165 @default.
• W2106548891 cites W2058209852 @default.
• W2106548891 cites W2064184667 @default.
• W2106548891 cites W2067431380 @default.
• W2106548891 cites W2082015820 @default.
• W2106548891 cites W2093475422 @default.
• W2106548891 cites W4293247451 @default.
• W2106548891 cites W74269732 @default.
• W2106548891 doi "https://doi.org/10.1016/s0022-2275(20)32073-3" @default.
• W2106548891 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10627501" @default.
• W2106548891 hasPublicationYear "2000" @default.
• W2106548891 type Work @default.
• W2106548891 sameAs 2106548891 @default.
• W2106548891 citedByCount "44" @default.
• W2106548891 countsByYear W21065488912012 @default.
• W2106548891 countsByYear W21065488912013 @default.
• W2106548891 countsByYear W21065488912014 @default.
• W2106548891 countsByYear W21065488912015 @default.
• W2106548891 countsByYear W21065488912016 @default.
• W2106548891 countsByYear W21065488912017 @default.
• W2106548891 countsByYear W21065488912018 @default.
• W2106548891 countsByYear W21065488912019 @default.
• W2106548891 countsByYear W21065488912020 @default.
• W2106548891 countsByYear W21065488912021 @default.
• W2106548891 countsByYear W21065488912022 @default.
• W2106548891 countsByYear W21065488912023 @default.
• W2106548891 crossrefType "journal-article" @default.
• W2106548891 hasAuthorship W2106548891A5033268514 @default.
• W2106548891 hasAuthorship W2106548891A5043775067 @default.
• W2106548891 hasBestOaLocation W21065488911 @default.
• W2106548891 hasConcept C104317684 @default.
• W2106548891 hasConcept C127947577 @default.
• W2106548891 hasConcept C140985366 @default.
• W2106548891 hasConcept C143065580 @default.
• W2106548891 hasConcept C171089720 @default.
• W2106548891 hasConcept C175106660 @default.
• W2106548891 hasConcept C181199279 @default.
• W2106548891 hasConcept C185592680 @default.
• W2106548891 hasConcept C2779697368 @default.
• W2106548891 hasConcept C2780716463 @default.
• W2106548891 hasConcept C30765947 @default.
• W2106548891 hasConcept C41183919 @default.
• W2106548891 hasConcept C55493867 @default.
• W2106548891 hasConceptScore W2106548891C104317684 @default.
• W2106548891 hasConceptScore W2106548891C127947577 @default.
• W2106548891 hasConceptScore W2106548891C140985366 @default.
• W2106548891 hasConceptScore W2106548891C143065580 @default.
• W2106548891 hasConceptScore W2106548891C171089720 @default.
• W2106548891 hasConceptScore W2106548891C175106660 @default.
• W2106548891 hasConceptScore W2106548891C181199279 @default.
• W2106548891 hasConceptScore W2106548891C185592680 @default.
• W2106548891 hasConceptScore W2106548891C2779697368 @default.
• W2106548891 hasConceptScore W2106548891C2780716463 @default.
• W2106548891 hasConceptScore W2106548891C30765947 @default.
• W2106548891 hasConceptScore W2106548891C41183919 @default.
• W2106548891 hasConceptScore W2106548891C55493867 @default.
• W2106548891 hasIssue "1" @default.
• W2106548891 hasLocation W21065488911 @default.
• W2106548891 hasOpenAccess W2106548891 @default.
• W2106548891 hasPrimaryLocation W21065488911 @default.
• W2106548891 hasRelatedWork W1528115394 @default.
• W2106548891 hasRelatedWork W1859800025 @default.
• W2106548891 hasRelatedWork W1962611077 @default.
• W2106548891 hasRelatedWork W1982522926 @default.
• W2106548891 hasRelatedWork W1983071870 @default.
• W2106548891 hasRelatedWork W1991615911 @default.
• W2106548891 hasRelatedWork W2056185138 @default.
• W2106548891 hasRelatedWork W2091223506 @default.
• W2106548891 hasRelatedWork W2115731835 @default.
• W2106548891 hasRelatedWork W2276923771 @default.
• W2106548891 hasVolume "41" @default.
• W2106548891 isParatext "false" @default.
• W2106548891 isRetracted "false" @default.

• next