FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106568364> ?p ?o ?g. }

• W2106568364 endingPage "654" @default.
• W2106568364 startingPage "629" @default.
• W2106568364 abstract "Summary We report here a broad analysis of the excretory/secretory (E/S) products of adult Brugia malayi, collected by in–vitro cultivation of the parasite. Culture media and conditions were optimized, and non–essential amino acids were found to be crucial for efficient protein synthesis under cell– and serum–free culture conditions. A close correlation was found between total protein secretion, phosphorylcholine–bearing antigen release and lactate production on each day of culture, indicating that E/S molecules are actively secreted. Parasites cultured in vitro take 2–3 days to adjust to the new environment, and show peak levels of secretion at days 3 and 4. The active secretion of phosphorylcholine by the parasite therefore justifies the measurement of this molecule as an indication of active infection, possibly reflecting total worm burdens. By comparing metaboli–cally labelled E/S from male and female worms, several molecules of low mol. wt, namely 10000, 13000, 14 000 and 22 000, together with high mol. wt components of above 12000 were found to be female specific. Tracing the origin of the E/S products, several molecules were also found to be associated with the surface. Among these, there are at least two glycoproteins, 29 000 and 51000 of which the 29000 molecule is a major surface protein. The immunogenicity of the E/S was examined and antigenic cross–reactivity was found with sera from most filarial infections but not with non–filarial nematodiases such as hookworm or Trichi–. nella. However, two molecules of low mol. wt, 15 000 and 19000, were not recognized by anti–Onchocerca sera and appeared to be potential Brugia–specific diagnostic molecules. Possible functional roles of the adult E/S products were examined but we could find no evidence of protease activity in the E/S or glutathione S–transferase activity in either the E/S or in whole somatic extract." @default.
• W2106568364 created "2016-06-24" @default.
• W2106568364 creator A5005805901 @default.
• W2106568364 creator A5012892998 @default.
• W2106568364 creator A5029260456 @default.
• W2106568364 creator A5032064825 @default.
• W2106568364 creator A5037852142 @default.
• W2106568364 date "1989-11-01" @default.
• W2106568364 modified "2023-09-30" @default.
• W2106568364 title "Secreted antigens of filarial nematodes: a survey and characterization of in vitro excreted/secreted products of adult Brugia malayi" @default.
• W2106568364 cites W1490226901 @default.
• W2106568364 cites W1493036774 @default.
• W2106568364 cites W1549705017 @default.
• W2106568364 cites W1570630012 @default.
• W2106568364 cites W1581414347 @default.
• W2106568364 cites W1965132098 @default.
• W2106568364 cites W1965748505 @default.
• W2106568364 cites W1968738962 @default.
• W2106568364 cites W1968766357 @default.
• W2106568364 cites W1974345115 @default.
• W2106568364 cites W1975298589 @default.
• W2106568364 cites W1988247703 @default.
• W2106568364 cites W1990635550 @default.
• W2106568364 cites W1995493144 @default.
• W2106568364 cites W2001607819 @default.
• W2106568364 cites W2003367776 @default.
• W2106568364 cites W2009053999 @default.
• W2106568364 cites W2015324455 @default.
• W2106568364 cites W2017594050 @default.
• W2106568364 cites W2018706813 @default.
• W2106568364 cites W2018980190 @default.
• W2106568364 cites W2021844965 @default.
• W2106568364 cites W2022842834 @default.
• W2106568364 cites W2027393724 @default.
• W2106568364 cites W2031119708 @default.
• W2106568364 cites W2032664717 @default.
• W2106568364 cites W2037102375 @default.
• W2106568364 cites W2041803234 @default.
• W2106568364 cites W2052084054 @default.
• W2106568364 cites W2053684851 @default.
• W2106568364 cites W2055669408 @default.
• W2106568364 cites W2070010734 @default.
• W2106568364 cites W2071225235 @default.
• W2106568364 cites W2072388873 @default.
• W2106568364 cites W2080363074 @default.
• W2106568364 cites W2083453910 @default.
• W2106568364 cites W2090870669 @default.
• W2106568364 cites W2092267744 @default.
• W2106568364 cites W2095098448 @default.
• W2106568364 cites W2096819129 @default.
• W2106568364 cites W2100837269 @default.
• W2106568364 cites W2128635872 @default.
• W2106568364 cites W2325618265 @default.
• W2106568364 cites W2328813281 @default.
• W2106568364 cites W2417021228 @default.
• W2106568364 cites W258376682 @default.
• W2106568364 cites W4256333067 @default.
• W2106568364 cites W2005192809 @default.
• W2106568364 doi "https://doi.org/10.1111/j.1365-3024.1989.tb00926.x" @default.
• W2106568364 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2616192" @default.
• W2106568364 hasPublicationYear "1989" @default.
• W2106568364 type Work @default.
• W2106568364 sameAs 2106568364 @default.
• W2106568364 citedByCount "32" @default.
• W2106568364 countsByYear W21065683642016 @default.
• W2106568364 countsByYear W21065683642020 @default.
• W2106568364 countsByYear W21065683642021 @default.
• W2106568364 crossrefType "journal-article" @default.
• W2106568364 hasAuthorship W2106568364A5005805901 @default.
• W2106568364 hasAuthorship W2106568364A5012892998 @default.
• W2106568364 hasAuthorship W2106568364A5029260456 @default.
• W2106568364 hasAuthorship W2106568364A5032064825 @default.
• W2106568364 hasAuthorship W2106568364A5037852142 @default.
• W2106568364 hasConcept C108625454 @default.
• W2106568364 hasConcept C136764020 @default.
• W2106568364 hasConcept C147483822 @default.
• W2106568364 hasConcept C156399914 @default.
• W2106568364 hasConcept C165901193 @default.
• W2106568364 hasConcept C202751555 @default.
• W2106568364 hasConcept C203014093 @default.
• W2106568364 hasConcept C2775848585 @default.
• W2106568364 hasConcept C2776833585 @default.
• W2106568364 hasConcept C2777629521 @default.
• W2106568364 hasConcept C41008148 @default.
• W2106568364 hasConcept C49039625 @default.
• W2106568364 hasConcept C55493867 @default.
• W2106568364 hasConcept C71928629 @default.
• W2106568364 hasConcept C86803240 @default.
• W2106568364 hasConcept C89423630 @default.
• W2106568364 hasConceptScore W2106568364C108625454 @default.
• W2106568364 hasConceptScore W2106568364C136764020 @default.
• W2106568364 hasConceptScore W2106568364C147483822 @default.
• W2106568364 hasConceptScore W2106568364C156399914 @default.
• W2106568364 hasConceptScore W2106568364C165901193 @default.
• W2106568364 hasConceptScore W2106568364C202751555 @default.
• W2106568364 hasConceptScore W2106568364C203014093 @default.
• W2106568364 hasConceptScore W2106568364C2775848585 @default.
• W2106568364 hasConceptScore W2106568364C2776833585 @default.

• next