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• W2106711360 abstract "Cells in the mammalian blastocyst segregate into three distinct lineages, namely, trophoblast, hypoblast, and epiblast. During development, these will form extraembryonic and embryonic tissues, respectively. In mouse, only epiblast cells can be directly converted into cultured pluripotent embryonic stem cells, capable of forming all adult cell types. This conversion is promoted by the double inhibition (i.e., 2i) of mitogen-activated protein kinase kinase (Map2k), antagonizing Fgf signaling, and of glycogen synthase kinase 3 (Gsk3), stimulating the Wnt pathway. We investigated the effect of 2i treatment on lineage segregation and pluripotency-related gene expression in bovine blastocysts. In vitro fertilized (IVF) embryos were cultured in the presence of dimethyl sulfoxide or inhibitors of MAP2K (0.4 μM PD0325901) and GSK3 (3 μM CHIR99021) from the zygote (Day 1) stage. Compared to vehicle controls, 2i conditions increased the abundance of cumulus cells in bovine IVF cultures, which compromised blastocyst formation. Following cumulus removal, 2i accelerated blastocyst development and increased inner cell mass (ICM) and trophoblast cell numbers by 30% and 27%, respectively. These developmental and morphological changes were accompanied by alterations in gene expression. Signal inhibition increased transcription of putative epiblast markers NANOG and SOX2 while repressing putative hypoblast marker GATA4. Using microsurgical blastocyst dissection, we found that the increase in NANOG and SOX2 levels was specific to the ICM and not due to ectopic expression in the trophoblast. Expression of other pluripotency-related (POU5F1, KLF4, DPPA3) or trophoblast-enriched (CDX2) genes was not affected. In summary, 2i conditions reprogrammed the transcriptional profile of bovine ICM but not trophoblast cells. By shifting the balance from hypoblast- to epiblast-associated gene expression, 2i culture may prime bovine epiblast for subsequent derivation of pluripotent stem cell cultures." @default.
• W2106711360 created "2016-06-24" @default.
• W2106711360 creator A5010055049 @default.
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• W2106711360 date "2013-03-01" @default.
• W2106711360 modified "2023-10-15" @default.
• W2106711360 title "Inhibition of MAP2K and GSK3 Signaling Promotes Bovine Blastocyst Development and Epiblast-Associated Expression of Pluripotency Factors1" @default.
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• W2106711360 cites W1972343960 @default.
• W2106711360 cites W1974365957 @default.
• W2106711360 cites W1977505187 @default.
• W2106711360 cites W1981088384 @default.
• W2106711360 cites W1982331566 @default.
• W2106711360 cites W1983272142 @default.
• W2106711360 cites W1985748545 @default.
• W2106711360 cites W1989436729 @default.
• W2106711360 cites W1990180850 @default.
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• W2106711360 cites W1992663780 @default.
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• W2106711360 cites W1999601272 @default.
• W2106711360 cites W2001073369 @default.
• W2106711360 cites W2006499356 @default.
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• W2106711360 cites W2017703255 @default.
• W2106711360 cites W2018765068 @default.
• W2106711360 cites W2020965347 @default.
• W2106711360 cites W2027200579 @default.
• W2106711360 cites W2032942434 @default.
• W2106711360 cites W2036326229 @default.
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• W2106711360 cites W2042231893 @default.
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• W2106711360 cites W2123829995 @default.
• W2106711360 cites W2126851387 @default.
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• W2106711360 cites W2132555347 @default.
• W2106711360 cites W2133200532 @default.
• W2106711360 cites W2134030824 @default.
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• W2106711360 doi "https://doi.org/10.1095/biolreprod.112.103390" @default.
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• W2106711360 hasPublicationYear "2013" @default.
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