FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106867111> ?p ?o ?g. }

• W2106867111 endingPage "133" @default.
• W2106867111 startingPage "121" @default.
• W2106867111 abstract "The objective of this study was to evaluate the effect of insect larval feeding on the fate and genetic transformability of recombinant DNA from a transplastomic plant. Leaves of tobacco plants with an aadA antibiotic resistance gene inserted into their chloroplast genome were incubated with larvae of the tobacco hornworm Manduca sexta (Lepidoptera). The specifically designed Acinetobacter strain BD413 pBAB2 was chosen to analyze the functional integrity of the aadA transgene for natural transformation after gut passages. No gene transfer was detected after simultaneous feeding of leaves and the Acinetobacter BD413 pBAB2 as a recipient, even though 15% of ingested Acinetobacter BD413 cells could be recovered as viable cells from feces 6 h after feeding. Results with real-time PCR indicated that an average of 98.2 to 99.99% of the aadA gene was degraded during the gut passage, but the range in the number of aadA genes in feces of larvae fed with transplastomic leaves was enormous, varying from 5 × 106 to 1 × 109 copies.g-1. DNA extracted from feces of larvae fed with transplastomic leaves was still able to transform externally added competent Acinetobacter BD413 pBAB2 in vitro. Transformation frequencies with concentrated feces DNA were in the same range as those found with leaves (10-4–10-6 transformants per recipient) or purified plasmid DNA (10-3–10-7). The presence of functionally intact DNA was also qualitatively observed after incubation of 30 mg freshly shed feces directly with competent Acinetobacter BD413 pBAB2, demonstrating that aadA genes in feces have a potential to undergo further horizontal gene transfer under environmental conditions." @default.
• W2106867111 created "2016-06-24" @default.
• W2106867111 creator A5059984692 @default.
• W2106867111 creator A5088473813 @default.
• W2106867111 date "2007-01-01" @default.
• W2106867111 modified "2023-09-23" @default.
• W2106867111 title "Leaf-feeding larvae of<i>Manduca sexta</i>(Insecta, Lepidoptera) drastically reduce copy numbers of<i>aadA</i>antibiotic resistance genes from transplastomic tobacco but maintain intact<i>aadA</i>genes in their feces" @default.
• W2106867111 cites W1582413449 @default.
• W2106867111 cites W1899687008 @default.
• W2106867111 cites W1977537416 @default.
• W2106867111 cites W1982486848 @default.
• W2106867111 cites W1982851306 @default.
• W2106867111 cites W1989126791 @default.
• W2106867111 cites W1995923687 @default.
• W2106867111 cites W2008370788 @default.
• W2106867111 cites W2009746333 @default.
• W2106867111 cites W2010931021 @default.
• W2106867111 cites W2027486968 @default.
• W2106867111 cites W2049619932 @default.
• W2106867111 cites W2050802703 @default.
• W2106867111 cites W2060460206 @default.
• W2106867111 cites W2060617312 @default.
• W2106867111 cites W2063349298 @default.
• W2106867111 cites W2072476917 @default.
• W2106867111 cites W2088057086 @default.
• W2106867111 cites W2088524973 @default.
• W2106867111 cites W2099202697 @default.
• W2106867111 cites W2123944159 @default.
• W2106867111 cites W2126154952 @default.
• W2106867111 cites W2131095547 @default.
• W2106867111 cites W2140212187 @default.
• W2106867111 cites W2141015650 @default.
• W2106867111 cites W2146219846 @default.
• W2106867111 cites W2151895705 @default.
• W2106867111 cites W2162158529 @default.
• W2106867111 cites W2166527836 @default.
• W2106867111 cites W2167199448 @default.
• W2106867111 cites W2169576181 @default.
• W2106867111 cites W2223518195 @default.
• W2106867111 cites W2268063248 @default.
• W2106867111 cites W2346556209 @default.
• W2106867111 cites W2463804924 @default.
• W2106867111 cites W4239054554 @default.
• W2106867111 cites W4249596264 @default.
• W2106867111 cites W4249690974 @default.
• W2106867111 doi "https://doi.org/10.1051/ebr:2007028" @default.
• W2106867111 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17961486" @default.
• W2106867111 hasPublicationYear "2007" @default.
• W2106867111 type Work @default.
• W2106867111 sameAs 2106867111 @default.
• W2106867111 citedByCount "13" @default.
• W2106867111 countsByYear W21068671112013 @default.
• W2106867111 countsByYear W21068671112016 @default.
• W2106867111 countsByYear W21068671112017 @default.
• W2106867111 countsByYear W21068671112021 @default.
• W2106867111 countsByYear W21068671112022 @default.
• W2106867111 crossrefType "journal-article" @default.
• W2106867111 hasAuthorship W2106867111A5059984692 @default.
• W2106867111 hasAuthorship W2106867111A5088473813 @default.
• W2106867111 hasBestOaLocation W21068671111 @default.
• W2106867111 hasConcept C104317684 @default.
• W2106867111 hasConcept C173758957 @default.
• W2106867111 hasConcept C177756618 @default.
• W2106867111 hasConcept C204241405 @default.
• W2106867111 hasConcept C22744801 @default.
• W2106867111 hasConcept C2776071262 @default.
• W2106867111 hasConcept C2780312687 @default.
• W2106867111 hasConcept C54355233 @default.
• W2106867111 hasConcept C59822182 @default.
• W2106867111 hasConcept C61716771 @default.
• W2106867111 hasConcept C86803240 @default.
• W2106867111 hasConcept C89423630 @default.
• W2106867111 hasConceptScore W2106867111C104317684 @default.
• W2106867111 hasConceptScore W2106867111C173758957 @default.
• W2106867111 hasConceptScore W2106867111C177756618 @default.
• W2106867111 hasConceptScore W2106867111C204241405 @default.
• W2106867111 hasConceptScore W2106867111C22744801 @default.
• W2106867111 hasConceptScore W2106867111C2776071262 @default.
• W2106867111 hasConceptScore W2106867111C2780312687 @default.
• W2106867111 hasConceptScore W2106867111C54355233 @default.
• W2106867111 hasConceptScore W2106867111C59822182 @default.
• W2106867111 hasConceptScore W2106867111C61716771 @default.
• W2106867111 hasConceptScore W2106867111C86803240 @default.
• W2106867111 hasConceptScore W2106867111C89423630 @default.
• W2106867111 hasIssue "1-2" @default.
• W2106867111 hasLocation W21068671111 @default.
• W2106867111 hasLocation W21068671112 @default.
• W2106867111 hasOpenAccess W2106867111 @default.
• W2106867111 hasPrimaryLocation W21068671111 @default.
• W2106867111 hasRelatedWork W2007904255 @default.
• W2106867111 hasRelatedWork W2044200937 @default.
• W2106867111 hasRelatedWork W2052710835 @default.
• W2106867111 hasRelatedWork W2112082204 @default.
• W2106867111 hasRelatedWork W2320099147 @default.
• W2106867111 hasRelatedWork W2769030298 @default.
• W2106867111 hasRelatedWork W2773528115 @default.
• W2106867111 hasRelatedWork W3028512630 @default.
• W2106867111 hasRelatedWork W4232080345 @default.

• next