Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106965174> ?p ?o ?g. }
- W2106965174 endingPage "2322" @default.
- W2106965174 startingPage "2316" @default.
- W2106965174 abstract "Here, we report a novel method consisting of capillary electrophoretic separation followed by two‐color LIF detection with postcolumn derivatization. The method can be used to discriminate glycoproteins in a protein mixture containing both glycosylated and unglycosylated proteins. The detector permitted simultaneous measurements of two electropherograms obtained by 450 nm (diode laser) and 532 nm (Nd:YAG laser) lasers excited native proteins following postcolumn derivatization with naphthalene‐2,3‐dicarboxaldehyde and concanavalin A (Con A) labeled with tetramethylrhodamine (rhodamine‐labeled Con A), respectively. So, a protein can be assigned as glycosylated if it shows a peak at the same migration time in both electropherograms. According to the proposed principle, in a single run we discriminated a glycosylated protein (thyroglobulin) from an unglycosylated protein (albumin) in the presence of rhodamine‐labeled Con A. Because the methodology permits the simultaneous detection of native proteins and their complexes with a fluorescently labeled probe, it should have broad applicability to binding assays." @default.
- W2106965174 created "2016-06-24" @default.
- W2106965174 creator A5053293073 @default.
- W2106965174 creator A5070684851 @default.
- W2106965174 date "2013-07-26" @default.
- W2106965174 modified "2023-09-30" @default.
- W2106965174 title "Discrimination of glycoproteins via two‐color laser‐induced fluorescence detection coupled with postcolumn derivatization in capillary electrophoresis" @default.
- W2106965174 cites W1612151898 @default.
- W2106965174 cites W1967961992 @default.
- W2106965174 cites W1972126018 @default.
- W2106965174 cites W1991045019 @default.
- W2106965174 cites W1994742101 @default.
- W2106965174 cites W1995913815 @default.
- W2106965174 cites W1998948607 @default.
- W2106965174 cites W2009739420 @default.
- W2106965174 cites W2014349811 @default.
- W2106965174 cites W2015147482 @default.
- W2106965174 cites W2022327561 @default.
- W2106965174 cites W2023778637 @default.
- W2106965174 cites W2024017427 @default.
- W2106965174 cites W2024893777 @default.
- W2106965174 cites W2027653329 @default.
- W2106965174 cites W2032679727 @default.
- W2106965174 cites W2034787930 @default.
- W2106965174 cites W2051092822 @default.
- W2106965174 cites W2058985131 @default.
- W2106965174 cites W2063507801 @default.
- W2106965174 cites W2064568709 @default.
- W2106965174 cites W2065419217 @default.
- W2106965174 cites W2067233371 @default.
- W2106965174 cites W2069278693 @default.
- W2106965174 cites W2079422209 @default.
- W2106965174 cites W2080851296 @default.
- W2106965174 cites W2083260383 @default.
- W2106965174 cites W2083949124 @default.
- W2106965174 cites W2091499280 @default.
- W2106965174 cites W2098601733 @default.
- W2106965174 cites W2104436843 @default.
- W2106965174 cites W2110907204 @default.
- W2106965174 cites W2114989907 @default.
- W2106965174 cites W2156979594 @default.
- W2106965174 cites W2160464134 @default.
- W2106965174 cites W2160594603 @default.
- W2106965174 cites W2181456498 @default.
- W2106965174 cites W81368696 @default.
- W2106965174 doi "https://doi.org/10.1002/elps.201300149" @default.
- W2106965174 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23775479" @default.
- W2106965174 hasPublicationYear "2013" @default.
- W2106965174 type Work @default.
- W2106965174 sameAs 2106965174 @default.
- W2106965174 citedByCount "4" @default.
- W2106965174 countsByYear W21069651742015 @default.
- W2106965174 countsByYear W21069651742016 @default.
- W2106965174 countsByYear W21069651742018 @default.
- W2106965174 countsByYear W21069651742023 @default.
- W2106965174 crossrefType "journal-article" @default.
- W2106965174 hasAuthorship W2106965174A5053293073 @default.
- W2106965174 hasAuthorship W2106965174A5070684851 @default.
- W2106965174 hasBestOaLocation W21069651742 @default.
- W2106965174 hasConcept C108625454 @default.
- W2106965174 hasConcept C121332964 @default.
- W2106965174 hasConcept C1342733 @default.
- W2106965174 hasConcept C162356407 @default.
- W2106965174 hasConcept C185592680 @default.
- W2106965174 hasConcept C2776923573 @default.
- W2106965174 hasConcept C2779916640 @default.
- W2106965174 hasConcept C2900643 @default.
- W2106965174 hasConcept C40250595 @default.
- W2106965174 hasConcept C43617362 @default.
- W2106965174 hasConcept C44995494 @default.
- W2106965174 hasConcept C55493867 @default.
- W2106965174 hasConcept C62520636 @default.
- W2106965174 hasConcept C91881484 @default.
- W2106965174 hasConceptScore W2106965174C108625454 @default.
- W2106965174 hasConceptScore W2106965174C121332964 @default.
- W2106965174 hasConceptScore W2106965174C1342733 @default.
- W2106965174 hasConceptScore W2106965174C162356407 @default.
- W2106965174 hasConceptScore W2106965174C185592680 @default.
- W2106965174 hasConceptScore W2106965174C2776923573 @default.
- W2106965174 hasConceptScore W2106965174C2779916640 @default.
- W2106965174 hasConceptScore W2106965174C2900643 @default.
- W2106965174 hasConceptScore W2106965174C40250595 @default.
- W2106965174 hasConceptScore W2106965174C43617362 @default.
- W2106965174 hasConceptScore W2106965174C44995494 @default.
- W2106965174 hasConceptScore W2106965174C55493867 @default.
- W2106965174 hasConceptScore W2106965174C62520636 @default.
- W2106965174 hasConceptScore W2106965174C91881484 @default.
- W2106965174 hasIssue "16" @default.
- W2106965174 hasLocation W21069651741 @default.
- W2106965174 hasLocation W21069651742 @default.
- W2106965174 hasLocation W21069651743 @default.
- W2106965174 hasOpenAccess W2106965174 @default.
- W2106965174 hasPrimaryLocation W21069651741 @default.
- W2106965174 hasRelatedWork W1973404691 @default.
- W2106965174 hasRelatedWork W1977308121 @default.
- W2106965174 hasRelatedWork W1979341406 @default.
- W2106965174 hasRelatedWork W2011407426 @default.
- W2106965174 hasRelatedWork W2064148978 @default.