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• W2107074454 abstract "To study the effect of glucose on sodium butyrate-induced proliferation inhibition and apoptosis in HT-29 cell line, and explored its possible mechanisms.HT-29 cells were grown in RPMI-1640 medium supplemented with 10% fetal calf serum, and were allowed to adhere for 24 h, and then replaced with experimental medium. Cell survival rates were detected by MTT assay. Apoptosis was detected by TUNEL assay. Glucose transport protein 1 (GLUT1) and monocarboxylate transporter 1 (MCT1) mRNA expression was detected by RT-PCR.Low concentration of glucose induced apoptosis and regulated proliferation in HT-29 cell line, and glucose can obviously inhibit the effect of proliferation inhibition and apoptosis induced by sodium butyrate. Glucose also down-regulated the expression of MCT1mRNA (0.28 +/- 0.07 vs 0.19 +/- 0.10, P < 0.05), and decreased the expression of GLUT1mRNA slightly (0.18 +/- 0.04 vs 0.13 +/- 0.03, P < 0.05).Glucose can regulate the effect of proliferation inhibition and apoptosis induced by sodium butyrate and this influence may be associated with the intracellular concentration of glucose and sodium butyrate." @default.
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• W2107074454 date "2007-01-01" @default.
• W2107074454 modified "2023-09-26" @default.
• W2107074454 title "Possible mechanism for the regulation of glucose on proliferation, inhibition and apoptosis of colon cancer cells induced by sodium butyrate" @default.
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• W2107074454 doi "https://doi.org/10.3748/wjg.v13.i29.4015" @default.
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