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• W2107097505 abstract "Abstract— A UV-dosimeter has been developed for routine measurements which mainly weights the various components of the spectrum in relation to their damaging effects on a microorganism. For this purpose a biofilm was constructed, comprising dried spores of Bacillus subtilis (wild-type or DNA repair defective strain), immobilized on transparent polyester plastic sheets. After irradiation, the biofilm was incubated in a growth medium. The proteins, synthesized by the immobilized microorganisms after spore germination and several cell divisions, were stained and determined by photometry, giving a measure of the biological activity. The ”biologically effective dose“ was determined from a calibration curve. It reflects the dose equivalent to that of the calibration source producing the same effect. The UV-response of the biofilm is additive and follows the reciprocity law in the range of fluence rates investigated. The response is nearly independent of temperature within the range of -20°C to +70°C as well as of humidity between about 37 and 80% relative humidity. Storage for up to 9 months at <70% relative humidity and room temperature does not significantly influence the viability of the spores." @default.
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• W2107097505 date "1992-03-01" @default.
• W2107097505 modified "2023-09-23" @default.
• W2107097505 title "A BIOFILM USED AS ULTRAVIOLET-DOSIMETER" @default.
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• W2107097505 doi "https://doi.org/10.1111/j.1751-1097.1992.tb04252.x" @default.
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