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- W2107227120 abstract "The diagnosis of toxocarosis is based upon the demonstration of antibodies by ELISA methods,although cross-reaction with other ascarids may occur in populations from tropical areas. For thisreason, some authors proposed western blotting as a confirmatory test. The aim of this work was todevelop an immunoblotting in simpler technical conditions and to compare results with the ELISAtest. With this purpose sera from adults and children with sign and/or symptoms of toxocarosis andliving in the metropolitan area of Resistencia city (Northeast of Argentina) were studied. ELISA testwas performed and 120 positives and 60 negatives sera were selected and analyzed again byimmunoblotting. Positive samples and controls showed a WB pattern with six bands of 67.6 kDa, 55.6kDa, 43.9 kDa, 32.4 kDa, 26.6 kDa and 23.4 kDa, while negative controls from endemic and non-endemic areas of toxocarosis showed no bands. Out of the 180 samples studied, in 172 coincidentresults for both methods were obtained (95.6%), 6 ELISA negative samples were positive for WB(3.3%) and 2 ELISA positive samples resulted negative in the WB (1.1%). The immunoblotting techniquedescribed in this work may constitute an adequate method for the diagnosis of toxocarosis in subtropicalareas, particularly useful in cases with negative or low-titers ELISA test results and with signs orsymptoms of the infection." @default.
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- W2107227120 date "2005-12-01" @default.
- W2107227120 modified "2023-10-01" @default.
- W2107227120 title "Immunoblotting para diagnóstico de toxocarosis humana en un área subtropical" @default.
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- W2107227120 doi "https://doi.org/10.4067/s0717-77122005000200003" @default.
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