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• W2107237638 abstract "In this study, the potential of matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF ICMS) was investigated for the identification of clinical isolates. The isolates were analyzed at the species and strain level. Spectral identification by MALDI-TOF ICMS was performed for all strains, and compared with the results of sequencing of the internal transcribed spacers (ITS1 and ITS2), and the 5.8S rDNA region. PCR fingerprinting analysis using primers M13, (GACA)4, and (AC)10 was performed in order to assess the intra-specific variability of Trichophyton rubrum strains. The identification of strains at species level by MALDI-TOF ICMS was in agreement with the previously performed morphological and biochemical analysis. Sequence data confirmed spectral mass identification at species level. Intra-specific variability was assessed. Within the T. rubrum cluster, strains were distributed into smaller highly related sub-groups with a similarity values above 85%. MALDI-TOF ICMS was shown to be a rapid, low-cost and accurate alternative tool for the identification and strain typing of T. rubrum. En este estudio se investigó el potencial de matrix-assisted laser desorption/ionization time-of-flight intact cell mass (MALDI-TOF ICMS) para la identificación de aislados clínicos. Los aislados fueron analizados al nivel de especie y de cepa. Se realizó una identificación espectral mediante MALDI-TOF ICMS de todas las cepas que se comparó con los resultados de secuenciación de la región espaciadores transcritos internos (ITS1 e ITS2) y el ADNr 5,8S. Los análisis PCR fingerprinting usando los primers M13, (GACA)4 y (AC)10 se realizaron con la intención de evaluar la variabilidad intraespecífica de las cepas de Trichophyton rubrum. La identificación de las cepas al nivel de especie mediante MALDI-TOF ICMS concordó con la ID realizada retrospectivamente en el análisis morfológico y bioquímico. La secuencia de datos confirmó la identificación por espectro de masas a nivel de especie. Se evaluó la variabilidad intraespecífica. Dentro del clúster de T. rubrum, las cepas se distribuyeron en subgrupos menores y muy relacionados con valores de similaridad superiores a 85%. Se demuestra que MALDI-TOF ICMS es una herramienta alternativa rápida, de bajo coste y precisa para la identificación y tipificación de cepas de T. rubrum." @default.
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• W2107237638 date "2014-01-01" @default.
• W2107237638 modified "2023-09-23" @default.
• W2107237638 title "The use of MALDI-TOF ICMS as an alternative tool for Trichophyton rubrum identification and typing" @default.
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• W2107237638 doi "https://doi.org/10.1016/j.eimc.2013.01.009" @default.
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