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• W2107330312 abstract "ABSTRACT Cytophaga hutchinsonii is a widely distributed cellulolytic bacterium in the phylum Bacteroidetes . It can digest crystalline cellulose rapidly without free cellulases or cellulosomes. The mechanism of its cellulose utilization remains a mystery. We developed an efficient method based on a linear DNA double-crossover and FLP-FRT recombination system to obtain unmarked deletions of both single genes and large genomic fragments in C. hutchinsonii . Unmarked deletion of CHU _ 3237 ( porU ), an ortholog of the C-terminal signal peptidase of a type IX secretion system (T9SS), resulted in defects in colony spreading, cellulose degradation, and protein secretion, indicating that it is a component of the T9SS and that T9SS plays an important role in cellulose degradation by C. hutchinsonii . Furthermore, deletions of four large genomic fragments were obtained using our method, and the sizes of the excised fragments varied from 9 to 19 kb, spanning from 6 to 22 genes. The customized FLP-FRT method provides an efficient tool for more rapid progress in the cellulose degradation mechanism and other physiological aspects of C. hutchinsonii ." @default.
• W2107330312 created "2016-06-24" @default.
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• W2107330312 date "2014-10-01" @default.
• W2107330312 modified "2023-10-16" @default.
• W2107330312 title "FLP-FRT-Based Method To Obtain Unmarked Deletions of <i>CHU</i> _ <i>3237</i> ( <i>porU</i> ) and Large Genomic Fragments of Cytophaga hutchinsonii" @default.
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• W2107330312 doi "https://doi.org/10.1128/aem.01785-14" @default.
• W2107330312 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4178665" @default.
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