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• W2107595417 abstract "Abstract The G‐quadruplex (G4) is a non‐canonical nucleic acid structure which regulates important cellular processes. RNA G4s have recently been shown to exist in human cells and be biologically significant. Described herein is a new approach to detect and map RNA G4s in cellular transcripts. This method exploits the specific control of RNA G4–cation and RNA G4–ligand interactions during reverse transcription, by using a selective reverse transcriptase to monitor RNA G4‐mediated reverse transcriptase stalling (RTS) events. Importantly, a ligation‐amplification strategy is coupled with RTS, and enables detection and mapping of G4s in important, low‐abundance cellular RNAs. Strong evidence is provided for G4 formation in full‐length cellular human telomerase RNA, offering important insights into its cellular function." @default.
• W2107595417 created "2016-06-24" @default.
• W2107595417 creator A5017879021 @default.
• W2107595417 creator A5040551102 @default.
• W2107595417 date "2015-04-23" @default.
• W2107595417 modified "2023-10-18" @default.
• W2107595417 title "Targeted Detection of G-Quadruplexes in Cellular RNAs" @default.
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• W2107595417 doi "https://doi.org/10.1002/anie.201500891" @default.
• W2107595417 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4510783" @default.
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