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- W2107698477 abstract "The present study evaluated the effects of heat stress on the ram seminal plasma proteome. Six Morada Nova rams were scrotal insulated for 8 days. Scrotal circumference, sperm parameters, and seminal fluid proteins were evaluated before (Day 0) and twice during scrotal insulation (Days 4 and 8), and weekly until semen parameters returned to preinsulation values (normal). Seminal proteins were analyzed by two-dimensional SDS-PAGE and mass spectrometry. Scrotal circumference decreased from 30 ± 0.4 cm on Day 0 to 22.6 ± 0.6 cm on Day 36 (P < 0.05) and became equivalent to preinsulation values on Day 71. Motile sperm became nearly absent from Day 8 to Day 64 but returned to normal on Day 113. Percentage of normal sperm changed similarly and returned to normal on Day 106. Rams were azoospermic between Days 29 and 64, and sperm concentration came back to normal on Day 92. The number of spots/two-dimensional gel reduced from 256 ± 31 on Day 0 to 104 ± 14 on Day 29 (when rams were azoospermic) and then increased to 183 ± 9 on Day 113 (P < 0.05), similar to spot counts before insulation. The intensities of 24 spots, referring to 17 seminal plasma proteins, were affected by treatment (P < 0.05). After insulation, seminal plasma had greater expression of actin (two isoforms), albumin, heat shock protein 70 kDa, protein DJ-1, HRPE773-like, C-reactive protein precursor, bodhesin-2 (one isoform), spermadhesins. Most protein spots had the greatest intensity between Days 8 and 29, returning to preinsulation values on Day 113 (when many sperm criteria returned to normal). Proteins downregulated after scrotal insulation included dipeptidyl peptidase 3, isoforms of heat shock protein 90 kDa, RSVP22, MMP2 and of Bdh2. In this case, RSVP22 was reduced on Day 113 and all others, on Day 134. Expression of MMP2 and HSP90.1 was reduced throughout the study. Integrin β5, V-type H+-ATPase subunit A, ZBTB 42-like protein, isoforms of Bdh2, PSP-I, and RSVP22 were upregulated after testis insulation. Intensities of these spots were maximum (P < 0.05) 8 days after insulation started or on Day 29. Expression of most of such proteins returned to normal on Day 113. In conclusion, scrotal insulation affected testis and sperm parameters of rams, indicating alterations in both spermatogenesis and sperm maturation. Changes of seminal plasma proteome were coincidental with variations in semen parameters. Proteins affected by heat challenge are potentially involved in sperm protection, maturation, and fertilization." @default.
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- W2107698477 date "2015-11-01" @default.
- W2107698477 modified "2023-09-22" @default.
- W2107698477 title "Effect of increased testicular temperature on seminal plasma proteome of the ram" @default.
- W2107698477 cites W1972889589 @default.
- W2107698477 cites W1975493095 @default.
- W2107698477 cites W1978218993 @default.
- W2107698477 cites W1978313308 @default.
- W2107698477 cites W1981546800 @default.
- W2107698477 cites W1983662609 @default.
- W2107698477 cites W1985393225 @default.
- W2107698477 cites W1985854006 @default.
- W2107698477 cites W1987106431 @default.
- W2107698477 cites W1987926476 @default.
- W2107698477 cites W1992117628 @default.
- W2107698477 cites W1992958573 @default.
- W2107698477 cites W1993246122 @default.
- W2107698477 cites W1994809016 @default.
- W2107698477 cites W1998241496 @default.
- W2107698477 cites W1998966201 @default.
- W2107698477 cites W2003618993 @default.
- W2107698477 cites W2004995471 @default.
- W2107698477 cites W2007790334 @default.
- W2107698477 cites W2010586194 @default.
- W2107698477 cites W2022720511 @default.
- W2107698477 cites W2038032661 @default.
- W2107698477 cites W2038086488 @default.
- W2107698477 cites W2041477841 @default.
- W2107698477 cites W2046726975 @default.
- W2107698477 cites W2047013333 @default.
- W2107698477 cites W2051133969 @default.
- W2107698477 cites W2053250093 @default.
- W2107698477 cites W2057987372 @default.
- W2107698477 cites W2058883374 @default.
- W2107698477 cites W2059117123 @default.
- W2107698477 cites W2059766228 @default.
- W2107698477 cites W206041396 @default.
- W2107698477 cites W2061514092 @default.
- W2107698477 cites W2062086261 @default.
- W2107698477 cites W2064514248 @default.
- W2107698477 cites W2065861852 @default.
- W2107698477 cites W2070407562 @default.
- W2107698477 cites W2073371105 @default.
- W2107698477 cites W2075437609 @default.
- W2107698477 cites W2076981817 @default.
- W2107698477 cites W2081402053 @default.
- W2107698477 cites W2082624234 @default.
- W2107698477 cites W2087094729 @default.
- W2107698477 cites W2099860833 @default.
- W2107698477 cites W2101381149 @default.
- W2107698477 cites W2102017305 @default.
- W2107698477 cites W2110475626 @default.
- W2107698477 cites W2117006560 @default.
- W2107698477 cites W2117163228 @default.
- W2107698477 cites W2120075368 @default.
- W2107698477 cites W2127787395 @default.
- W2107698477 cites W2128560987 @default.
- W2107698477 cites W2135957572 @default.
- W2107698477 cites W2142157062 @default.
- W2107698477 cites W2142901670 @default.
- W2107698477 cites W2144514579 @default.
- W2107698477 cites W2145981397 @default.
- W2107698477 cites W2148448950 @default.
- W2107698477 cites W2152400519 @default.
- W2107698477 cites W2153891800 @default.
- W2107698477 cites W2164578937 @default.
- W2107698477 cites W2170748127 @default.
- W2107698477 cites W4213141530 @default.
- W2107698477 cites W4293247451 @default.
- W2107698477 doi "https://doi.org/10.1016/j.theriogenology.2015.07.008" @default.
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