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- W2107698768 abstract "Bacillus circulans A11, an alkaline-tolerant cyclodextrin-metabolizing bacterium isolated from South-East Asian soil, was reidentified as Paenibacillus sp. A11 based on 16S rRNA gene sequence comparison, G + C content and cellular fatty acid composition. Levels of similarity of the 16S rRNA gene between strain A11 and the Paenibacillus species were 90–99%, while similarity with Bacillus circulans was only 86%. The major cellular fatty acid was anteiso-C15:0 which accounted for 59.3% of the total cellular fatty acids and the G+C content was 50.3 mol%. The CDase gene coding for this enzyme was cloned into E. coli. The open reading frame of the CDase gene was 1,959 bp encoding a CDase of 653 amino acid residues. At maximum growth, the specific activity of the recombinant CDase from E. coli was higher than that of Paenibacillus sp. A11. By SDS-PAGE, the translation product of the recombinant gene showed the same mobility as the purified CDase from the original strain. CDase from both Paenibacillus sp. A11 and E. coli produced glucose and maltose as dominant end-products of β-CD hydrolysis. The ratio of maltose to glucose was 1:2. (© 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)" @default.
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- W2107698768 date "2004-09-01" @default.
- W2107698768 modified "2023-09-23" @default.
- W2107698768 title "Identification of an alkaline-tolerant cyclodextrin-metabolizing bacterium and characterization of its cyclodextrinase gene" @default.
- W2107698768 doi "https://doi.org/10.1002/jobm.200410412" @default.
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