FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2107698932> ?p ?o ?g. }

• W2107698932 endingPage "400" @default.
• W2107698932 startingPage "396" @default.
• W2107698932 abstract "Enterobacter sakazakii is a widespread and life-threatening bacterium especially in polluted powdered infant milk formula. Several methods have been developed for detection of E. sakazakii such as physiological and biochemical methods, PCR and loop-mediated isothermal amplification. However, these procedures were disadvantages due to a long assay time, low sensitivity or the use of toxic reagents. Our method of cross-priming amplification (CPA) under isothermal conditions combined with immuno-blotting analysis made the whole detection procedure more sensitivity and lower time-consuming. A set of specific displacement primers, cross primers and testing primers were designed based on six specific sequences in E. sakazakii 16S-23S rDNA internal transcribed spacer. Under isothermal condition at 63 °C for 60 min, the specific amplification and hybridization steps were processed simultaneously. The specificity of the CPA was tested in panel of 54 different bacterial strains and 236 milk powder products. Two red signal lines were developed on the BioHelix Express strip in all of positive E. sakazakii strains, and only one signal line was demonstrated by non- E. sakazakii bacterial strains. The limit of decetion of CPA was 6.3 ± 2.7277 fg for the genomic DNA, 88 ± 8.7892 cfu/ml for pure bacterial culture, and 3.2 ± 2.0569 cfu per 100 g milk powder with pre-enrichment. The current study demonstrated that the assay method of CPA combined with immuno-blotting analysis was a specific and sensitive detection for the rapid detection of E. sakazakii." @default.
• W2107698932 created "2016-06-24" @default.
• W2107698932 creator A5033656534 @default.
• W2107698932 creator A5041234681 @default.
• W2107698932 creator A5045599932 @default.
• W2107698932 creator A5047651198 @default.
• W2107698932 creator A5047937524 @default.
• W2107698932 creator A5076084917 @default.
• W2107698932 date "2010-12-01" @default.
• W2107698932 modified "2023-09-26" @default.
• W2107698932 title "Rapid and sensitive detection of Enterobacter sakazakii by cross-priming amplification combined with immuno-blotting analysis" @default.
• W2107698932 cites W1941260612 @default.
• W2107698932 cites W1964520015 @default.
• W2107698932 cites W1989990305 @default.
• W2107698932 cites W1992622868 @default.
• W2107698932 cites W1997153901 @default.
• W2107698932 cites W2000635215 @default.
• W2107698932 cites W2005166906 @default.
• W2107698932 cites W2028782066 @default.
• W2107698932 cites W2039196334 @default.
• W2107698932 cites W2043590721 @default.
• W2107698932 cites W2044898162 @default.
• W2107698932 cites W2046810696 @default.
• W2107698932 cites W2051092843 @default.
• W2107698932 cites W2067979569 @default.
• W2107698932 cites W2071269610 @default.
• W2107698932 cites W2073697850 @default.
• W2107698932 cites W2100128068 @default.
• W2107698932 cites W2153917781 @default.
• W2107698932 cites W2155030659 @default.
• W2107698932 cites W2167026482 @default.
• W2107698932 cites W2168370293 @default.
• W2107698932 cites W2169787126 @default.
• W2107698932 cites W2299724385 @default.
• W2107698932 cites W4235630810 @default.
• W2107698932 doi "https://doi.org/10.1016/j.mcp.2010.09.001" @default.
• W2107698932 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20850522" @default.
• W2107698932 hasPublicationYear "2010" @default.
• W2107698932 type Work @default.
• W2107698932 sameAs 2107698932 @default.
• W2107698932 citedByCount "39" @default.
• W2107698932 countsByYear W21076989322012 @default.
• W2107698932 countsByYear W21076989322013 @default.
• W2107698932 countsByYear W21076989322014 @default.
• W2107698932 countsByYear W21076989322015 @default.
• W2107698932 countsByYear W21076989322016 @default.
• W2107698932 countsByYear W21076989322017 @default.
• W2107698932 countsByYear W21076989322018 @default.
• W2107698932 countsByYear W21076989322019 @default.
• W2107698932 countsByYear W21076989322020 @default.
• W2107698932 countsByYear W21076989322021 @default.
• W2107698932 countsByYear W21076989322022 @default.
• W2107698932 countsByYear W21076989322023 @default.
• W2107698932 crossrefType "journal-article" @default.
• W2107698932 hasAuthorship W2107698932A5033656534 @default.
• W2107698932 hasAuthorship W2107698932A5041234681 @default.
• W2107698932 hasAuthorship W2107698932A5045599932 @default.
• W2107698932 hasAuthorship W2107698932A5047651198 @default.
• W2107698932 hasAuthorship W2107698932A5047937524 @default.
• W2107698932 hasAuthorship W2107698932A5076084917 @default.
• W2107698932 hasConcept C104317684 @default.
• W2107698932 hasConcept C119128265 @default.
• W2107698932 hasConcept C153911025 @default.
• W2107698932 hasConcept C185592680 @default.
• W2107698932 hasConcept C2778983983 @default.
• W2107698932 hasConcept C43617362 @default.
• W2107698932 hasConcept C54355233 @default.
• W2107698932 hasConcept C547475151 @default.
• W2107698932 hasConcept C552990157 @default.
• W2107698932 hasConcept C60635243 @default.
• W2107698932 hasConcept C85265743 @default.
• W2107698932 hasConcept C86803240 @default.
• W2107698932 hasConcept C89423630 @default.
• W2107698932 hasConceptScore W2107698932C104317684 @default.
• W2107698932 hasConceptScore W2107698932C119128265 @default.
• W2107698932 hasConceptScore W2107698932C153911025 @default.
• W2107698932 hasConceptScore W2107698932C185592680 @default.
• W2107698932 hasConceptScore W2107698932C2778983983 @default.
• W2107698932 hasConceptScore W2107698932C43617362 @default.
• W2107698932 hasConceptScore W2107698932C54355233 @default.
• W2107698932 hasConceptScore W2107698932C547475151 @default.
• W2107698932 hasConceptScore W2107698932C552990157 @default.
• W2107698932 hasConceptScore W2107698932C60635243 @default.
• W2107698932 hasConceptScore W2107698932C85265743 @default.
• W2107698932 hasConceptScore W2107698932C86803240 @default.
• W2107698932 hasConceptScore W2107698932C89423630 @default.
• W2107698932 hasIssue "6" @default.
• W2107698932 hasLocation W21076989321 @default.
• W2107698932 hasLocation W21076989322 @default.
• W2107698932 hasOpenAccess W2107698932 @default.
• W2107698932 hasPrimaryLocation W21076989321 @default.
• W2107698932 hasRelatedWork W2268419274 @default.
• W2107698932 hasRelatedWork W2366402691 @default.
• W2107698932 hasRelatedWork W2368836504 @default.
• W2107698932 hasRelatedWork W2377082422 @default.
• W2107698932 hasRelatedWork W2378991221 @default.
• W2107698932 hasRelatedWork W2379707776 @default.
• W2107698932 hasRelatedWork W2383859492 @default.

• next