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• W2107704287 abstract "Huan-Nao-Yi-Cong-Fang is a potential specific traditional Chinese patent medicine for the treatment of Alzheimer's disease. It is composed of five herbal medicines: Radix Polygoni Multiflori Prepamousea, Panax ginseng, Ligusticum chuanxiong Hort, Coptis chinensis Franch and Acorus gramineus. A sensitive and effective UFLC-ESI/MS method has been developed for the simultaneous quantification of seven active compounds including ferulic acid, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, berberine hydrochloride, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, and emodin in Huan-Nao-Yi-Cong-Fang and rat serum. Samples were separated on a Shim-pack XR-ODS column (100 × 2.0 mm I.D., 5 μm) using acetonitrile–water containing 0.1% formic acid as the mobile phase and then ionized and detected by ESI/MS with selected ion monitoring in either positive or negative mode. All the calibration curves have good linear relationships with ng mL−1 levels of limits of quantification. The mean recoveries ranged from 87.06% to 106.79% with a relative standard deviation of <9.89%. The proposed method was further applied to the analysis of rat serum samples after oral administration of Huan-Nao-Yi-Cong-Fang. The results show that the established UFLC-ESI/MS method is a very useful tool for the quality control of Huan-Nao-Yi-Cong-Fang and pharmacokinetic research." @default.
• W2107704287 created "2016-06-24" @default.
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• W2107704287 date "2014-06-20" @default.
• W2107704287 modified "2023-10-15" @default.
• W2107704287 title "Simultaneous and sensitive quantification of seven active compounds in Huan-Nao-Yi-Cong-Fang and rat serum by UFLC-ESI/MS" @default.
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• W2107704287 doi "https://doi.org/10.1039/c4ay00840e" @default.
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