FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2107751372> ?p ?o ?g. }

• W2107751372 endingPage "1799" @default.
• W2107751372 startingPage "1789" @default.
• W2107751372 abstract "ABSTRACT A putative maltose/maltodextrin operon was found in the Sulfolobus acidocaldarius DSM639 genome. The gene cluster consisted of 7 genes ( malA , trmB , amyA , malG , malF , malE , and malK ). Here, we report the identification of MalA, which is responsible for the hydrolysis of maltose or maltodextrin to glucose in S. acidocaldarius . The transcription level of malA was increased 3-fold upon the addition of maltose or starch to the medium. Moreover, the α-glucosidase activity for maltose as a substrate in cell extracts of S. acidocaldarius DSM639 was also 11- and 10-fold higher during growth in YT medium (Brock's mineral salts, 0.1% [wt/vol] tryptone, and 0.005% [wt/vol] yeast extract) containing maltose or starch, respectively, than during growth on other sugars. The gene encoding MalA was cloned and expressed in S. acidocaldarius . The enzyme purified from the organism was a dodecamer in its active state and showed strong maltose-hydrolyzing activity at 100°C and pH 5.0. MalA was remarkably thermostable, with half-lives of 33.8 h, 10.6 h, and 1.8 h at 95°C, 100°C, and 105°C, respectively. Substrate specificity and kinetic studies of MalA with maltooligosaccharides indicated that MalA efficiently hydrolyzed maltose to maltopentaose, which is a typical characteristic of GH31-type α-glucosidases. However, glycogen or starch was not hydrolyzed. Reverse transcription-PCR, sugar uptake, and growth studies of the wild-type DSM639 and Δ malEFG mutant on different sugars demonstrated that MalA located in the mal operon gene cluster is involved in maltose and starch metabolism in S. acidocaldarius ." @default.
• W2107751372 created "2016-06-24" @default.
• W2107751372 creator A5024000478 @default.
• W2107751372 creator A5043692950 @default.
• W2107751372 creator A5066416698 @default.
• W2107751372 date "2013-04-15" @default.
• W2107751372 modified "2023-09-24" @default.
• W2107751372 title "Identification and Characterization of MalA in the Maltose/Maltodextrin Operon of Sulfolobus acidocaldarius DSM639" @default.
• W2107751372 cites W1532217983 @default.
• W2107751372 cites W1856872680 @default.
• W2107751372 cites W1968062298 @default.
• W2107751372 cites W1997146378 @default.
• W2107751372 cites W2000838625 @default.
• W2107751372 cites W2013516534 @default.
• W2107751372 cites W2020450170 @default.
• W2107751372 cites W2020659155 @default.
• W2107751372 cites W2025320572 @default.
• W2107751372 cites W2031059486 @default.
• W2107751372 cites W2031517834 @default.
• W2107751372 cites W2050433725 @default.
• W2107751372 cites W2057370325 @default.
• W2107751372 cites W2066498304 @default.
• W2107751372 cites W2068772472 @default.
• W2107751372 cites W2076107442 @default.
• W2107751372 cites W2083354414 @default.
• W2107751372 cites W2084498039 @default.
• W2107751372 cites W2087583350 @default.
• W2107751372 cites W2093004518 @default.
• W2107751372 cites W2096997987 @default.
• W2107751372 cites W2106435451 @default.
• W2107751372 cites W2126416255 @default.
• W2107751372 cites W2127849149 @default.
• W2107751372 cites W2128684194 @default.
• W2107751372 cites W2130047273 @default.
• W2107751372 cites W2138049414 @default.
• W2107751372 cites W2145442228 @default.
• W2107751372 cites W2158958137 @default.
• W2107751372 cites W4293247451 @default.
• W2107751372 doi "https://doi.org/10.1128/jb.01713-12" @default.
• W2107751372 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3624569" @default.
• W2107751372 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23396915" @default.
• W2107751372 hasPublicationYear "2013" @default.
• W2107751372 type Work @default.
• W2107751372 sameAs 2107751372 @default.
• W2107751372 citedByCount "22" @default.
• W2107751372 countsByYear W21077513722014 @default.
• W2107751372 countsByYear W21077513722015 @default.
• W2107751372 countsByYear W21077513722016 @default.
• W2107751372 countsByYear W21077513722017 @default.
• W2107751372 countsByYear W21077513722018 @default.
• W2107751372 countsByYear W21077513722020 @default.
• W2107751372 countsByYear W21077513722021 @default.
• W2107751372 countsByYear W21077513722022 @default.
• W2107751372 countsByYear W21077513722023 @default.
• W2107751372 crossrefType "journal-article" @default.
• W2107751372 hasAuthorship W2107751372A5024000478 @default.
• W2107751372 hasAuthorship W2107751372A5043692950 @default.
• W2107751372 hasAuthorship W2107751372A5066416698 @default.
• W2107751372 hasBestOaLocation W21077513721 @default.
• W2107751372 hasConcept C104317684 @default.
• W2107751372 hasConcept C143065580 @default.
• W2107751372 hasConcept C181199279 @default.
• W2107751372 hasConcept C203075996 @default.
• W2107751372 hasConcept C2778197599 @default.
• W2107751372 hasConcept C2780095022 @default.
• W2107751372 hasConcept C2781287210 @default.
• W2107751372 hasConcept C36857842 @default.
• W2107751372 hasConcept C529335014 @default.
• W2107751372 hasConcept C550995028 @default.
• W2107751372 hasConcept C55493867 @default.
• W2107751372 hasConcept C86803240 @default.
• W2107751372 hasConceptScore W2107751372C104317684 @default.
• W2107751372 hasConceptScore W2107751372C143065580 @default.
• W2107751372 hasConceptScore W2107751372C181199279 @default.
• W2107751372 hasConceptScore W2107751372C203075996 @default.
• W2107751372 hasConceptScore W2107751372C2778197599 @default.
• W2107751372 hasConceptScore W2107751372C2780095022 @default.
• W2107751372 hasConceptScore W2107751372C2781287210 @default.
• W2107751372 hasConceptScore W2107751372C36857842 @default.
• W2107751372 hasConceptScore W2107751372C529335014 @default.
• W2107751372 hasConceptScore W2107751372C550995028 @default.
• W2107751372 hasConceptScore W2107751372C55493867 @default.
• W2107751372 hasConceptScore W2107751372C86803240 @default.
• W2107751372 hasIssue "8" @default.
• W2107751372 hasLocation W21077513721 @default.
• W2107751372 hasLocation W21077513722 @default.
• W2107751372 hasLocation W21077513723 @default.
• W2107751372 hasLocation W21077513724 @default.
• W2107751372 hasOpenAccess W2107751372 @default.
• W2107751372 hasPrimaryLocation W21077513721 @default.
• W2107751372 hasRelatedWork W1503440502 @default.
• W2107751372 hasRelatedWork W1999291015 @default.
• W2107751372 hasRelatedWork W2008569598 @default.
• W2107751372 hasRelatedWork W2064051574 @default.
• W2107751372 hasRelatedWork W2107751372 @default.
• W2107751372 hasRelatedWork W2114656992 @default.
• W2107751372 hasRelatedWork W2171171862 @default.
• W2107751372 hasRelatedWork W2628139963 @default.

• next