FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2107758052> ?p ?o ?g. }

• W2107758052 abstract "Staphylococcus aureus (S. aureus) is a major nosocomial pathogen and the infection with this organism in human is increasing due to the spread of antibiotic resistant strains. One of the resistance mechanisms of S. aureus comprises modification in binding proteins to penicillin. Vaccine strategy may be useful in controlling the infections induced by this organism. This study aimed at developing and producing the recombinant protein PBP2a as a vaccine candidate and evaluating the related humoral immune response in a murine model.A 242 bp fragment of mecA gene was amplified by PCR from S. aureus COL strain and then cloned into prokaryotic expression vector pET-24a. For expression of recombinant protein, pET24a-mec plasmid was transformed into competent E. coli BL21 (DE3) cells. Recombinant protein was over expressed with 1 mM isopropythio-β-D-galctoside (IPTG) and purified using Ni-NTA agarose. SDS-PAGE and western blotting were carried out to confirm protein expression. For immunization of experimental groups, Balb/c mice were injected subcutaneously with 20 µg of recombinant PBP2a three times with three weeks intervals. The sera of experimental groups were collected three weeks after the last immunization and then specific antibodies were evaluated by ELISA method.Successful cloning of mecA was confirmed by colony-PCR, enzymatic digestion, and sequencing. SDS-PAGE and western blot analysis showed that recombinant protein with molecular weight of 13 kDa is over expressed. In addition, high titer of specific antibody against PBP2a in vaccinated mice was developed as compared with the control group and confirmed the immunogenicity of the vaccine candidate.Results suggest that PBP2a recombinant induced specific antibodies and can be used as Staphylococcal vaccine candidate after further studies." @default.
• W2107758052 created "2016-06-24" @default.
• W2107758052 creator A5021513156 @default.
• W2107758052 creator A5024066810 @default.
• W2107758052 creator A5038567292 @default.
• W2107758052 creator A5070567360 @default.
• W2107758052 creator A5084050163 @default.
• W2107758052 date "2013-10-01" @default.
• W2107758052 modified "2023-09-24" @default.
• W2107758052 title "Cloning, Expression and Purification of Penicillin Binding Protein2a (PBP2a) from Methicillin Resistant Staphylococcus aureus: A Study on Immunoreactivity in Balb/C Mouse." @default.
• W2107758052 cites W1558813989 @default.
• W2107758052 cites W1984039441 @default.
• W2107758052 cites W1999985155 @default.
• W2107758052 cites W2017252260 @default.
• W2107758052 cites W2032294136 @default.
• W2107758052 cites W2044628504 @default.
• W2107758052 cites W2051225320 @default.
• W2107758052 cites W2060893110 @default.
• W2107758052 cites W2086046084 @default.
• W2107758052 cites W2100419851 @default.
• W2107758052 cites W2109793735 @default.
• W2107758052 cites W2110830499 @default.
• W2107758052 cites W2113091465 @default.
• W2107758052 cites W2116607488 @default.
• W2107758052 cites W2119011878 @default.
• W2107758052 cites W2124126957 @default.
• W2107758052 cites W2130436333 @default.
• W2107758052 cites W2132682385 @default.
• W2107758052 cites W2147612191 @default.
• W2107758052 cites W2150923940 @default.
• W2107758052 cites W2154775956 @default.
• W2107758052 cites W2156502117 @default.
• W2107758052 cites W2166709011 @default.
• W2107758052 cites W2314649741 @default.
• W2107758052 cites W2623255807 @default.
• W2107758052 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3838764" @default.
• W2107758052 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24285994" @default.
• W2107758052 hasPublicationYear "2013" @default.
• W2107758052 type Work @default.
• W2107758052 sameAs 2107758052 @default.
• W2107758052 citedByCount "6" @default.
• W2107758052 countsByYear W21077580522015 @default.
• W2107758052 countsByYear W21077580522016 @default.
• W2107758052 countsByYear W21077580522018 @default.
• W2107758052 countsByYear W21077580522019 @default.
• W2107758052 countsByYear W21077580522022 @default.
• W2107758052 crossrefType "journal-article" @default.
• W2107758052 hasAuthorship W2107758052A5021513156 @default.
• W2107758052 hasAuthorship W2107758052A5024066810 @default.
• W2107758052 hasAuthorship W2107758052A5038567292 @default.
• W2107758052 hasAuthorship W2107758052A5070567360 @default.
• W2107758052 hasAuthorship W2107758052A5084050163 @default.
• W2107758052 hasConcept C104317684 @default.
• W2107758052 hasConcept C121050878 @default.
• W2107758052 hasConcept C153911025 @default.
• W2107758052 hasConcept C159047783 @default.
• W2107758052 hasConcept C159654299 @default.
• W2107758052 hasConcept C174802600 @default.
• W2107758052 hasConcept C199360897 @default.
• W2107758052 hasConcept C203014093 @default.
• W2107758052 hasConcept C2776415932 @default.
• W2107758052 hasConcept C2776653525 @default.
• W2107758052 hasConcept C2779489039 @default.
• W2107758052 hasConcept C40767141 @default.
• W2107758052 hasConcept C41008148 @default.
• W2107758052 hasConcept C501593827 @default.
• W2107758052 hasConcept C523546767 @default.
• W2107758052 hasConcept C54355233 @default.
• W2107758052 hasConcept C55493867 @default.
• W2107758052 hasConcept C85265743 @default.
• W2107758052 hasConcept C86803240 @default.
• W2107758052 hasConcept C89423630 @default.
• W2107758052 hasConceptScore W2107758052C104317684 @default.
• W2107758052 hasConceptScore W2107758052C121050878 @default.
• W2107758052 hasConceptScore W2107758052C153911025 @default.
• W2107758052 hasConceptScore W2107758052C159047783 @default.
• W2107758052 hasConceptScore W2107758052C159654299 @default.
• W2107758052 hasConceptScore W2107758052C174802600 @default.
• W2107758052 hasConceptScore W2107758052C199360897 @default.
• W2107758052 hasConceptScore W2107758052C203014093 @default.
• W2107758052 hasConceptScore W2107758052C2776415932 @default.
• W2107758052 hasConceptScore W2107758052C2776653525 @default.
• W2107758052 hasConceptScore W2107758052C2779489039 @default.
• W2107758052 hasConceptScore W2107758052C40767141 @default.
• W2107758052 hasConceptScore W2107758052C41008148 @default.
• W2107758052 hasConceptScore W2107758052C501593827 @default.
• W2107758052 hasConceptScore W2107758052C523546767 @default.
• W2107758052 hasConceptScore W2107758052C54355233 @default.
• W2107758052 hasConceptScore W2107758052C55493867 @default.
• W2107758052 hasConceptScore W2107758052C85265743 @default.
• W2107758052 hasConceptScore W2107758052C86803240 @default.
• W2107758052 hasConceptScore W2107758052C89423630 @default.
• W2107758052 hasLocation W21077580521 @default.
• W2107758052 hasOpenAccess W2107758052 @default.
• W2107758052 hasPrimaryLocation W21077580521 @default.
• W2107758052 hasRelatedWork W1489927095 @default.
• W2107758052 hasRelatedWork W2007133830 @default.
• W2107758052 hasRelatedWork W2342745674 @default.
• W2107758052 hasRelatedWork W2355669527 @default.
• W2107758052 hasRelatedWork W2361163271 @default.

• next