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- W2107792917 abstract "The sensitivity of human oocytes to cryodamage may compromise their developmental competence following cryopreservation. Herein, we compared the ultrastructure and the response to the calcium (Ca²⁺) ionophore A23187 of fresh, slow-frozen and vitrified metaphase II (MII) human oocytes.Supernumerary fresh MII oocytes, donated under written informed consent, were cryopreserved through either a slow cooling procedure based on propane-1,2-diol and 0.3 M sucrose or a closed vitrification system based on dimethylsulphoxide (DMSO) and ethylene glycol (EG). Ultrastructure of fresh and cryopreserved oocytes was assessed by transmission electron microscopy and compared through morphometrical analysis; intracellular calcium ([Ca²⁺](i)) dynamics was studied by evaluating the response to the Ca²⁺ ionophore A23187.Morphometric analysis demonstrated a markedly higher proportion of oocytes with large vacuoles, inward displacement of organelles from the pericortical toward the deep cytoplasm, and mitochondrial damage in slow-frozen compared with both fresh and vitrified oocytes. A23187 increased the [Ca²⁺](i) in all oocyte groups and the peak average increase in slow-frozen oocytes was significantly higher than in both fresh and vitrified oocytes. Moreover, the ability of slow-frozen oocytes to recover [Ca²⁺](i) to basal levels was significantly reduced compared with both fresh and vitrified oocytes.Closed vitrification based on DMSO and EG preserves the ultrastructural features and the ability to respond to the Ca²⁺ ionophore A23187 significantly better than does slow freezing with 0.3 M sucrose. Damage to organelles involved in the [Ca²⁺](i) modulation might reduce the developmental competence of cryopreserved oocytes." @default.
- W2107792917 created "2016-06-24" @default.
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- W2107792917 date "2011-06-29" @default.
- W2107792917 modified "2023-10-18" @default.
- W2107792917 title "Ultrastructure and intracellular calcium response during activation in vitrified and slow-frozen human oocytes" @default.
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- W2107792917 doi "https://doi.org/10.1093/humrep/der210" @default.
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