FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2107826964> ?p ?o ?g. }

• W2107826964 endingPage "25" @default.
• W2107826964 startingPage "25e" @default.
• W2107826964 abstract "The use of primary somatic cells in nuclear transfer procedure has opened a new opportunity to manipulate domestic animal genomes via homologous recombination. To date, while a few loci have been targeted in somatic cells using similar enrichment strategies as those used in mouse ES cells, there have been problems of low efficiency, mixed targeted and non-targeted cells within a colony and difficulties in cloning the cell after targeting. Utilizing the hypoxanthine guanine phosphoribosyl transferase (HPRT) as a test locus, it was determined that while no targeted colonies were identified using a conventional targeting construct, an average of 1 per million targeted cells were identified when a nuclear localization signal (nls) was added to the construct. When the nls was combined with cell synchronization using a thymidine block, targeting efficiency increased 7-fold. Moreover, the number of random integrants decreased by over 54-fold resulting in a 1:3 targeted to random integration ratio. This method should facilitate the application of homologous recombination to primary somatic cells." @default.
• W2107826964 created "2016-06-24" @default.
• W2107826964 creator A5030851843 @default.
• W2107826964 creator A5064912159 @default.
• W2107826964 date "2004-02-13" @default.
• W2107826964 modified "2023-09-28" @default.
• W2107826964 title "Nuclear localization signal and cell synchrony enhance gene targeting efficiency in primary fetal fibroblasts" @default.
• W2107826964 cites W1538506308 @default.
• W2107826964 cites W1597619535 @default.
• W2107826964 cites W1781623861 @default.
• W2107826964 cites W1826783215 @default.
• W2107826964 cites W1970356965 @default.
• W2107826964 cites W1970894097 @default.
• W2107826964 cites W1975844162 @default.
• W2107826964 cites W1978581150 @default.
• W2107826964 cites W1983237714 @default.
• W2107826964 cites W1992525863 @default.
• W2107826964 cites W1992934869 @default.
• W2107826964 cites W2008661813 @default.
• W2107826964 cites W2013379590 @default.
• W2107826964 cites W2014825046 @default.
• W2107826964 cites W2028408655 @default.
• W2107826964 cites W2058755882 @default.
• W2107826964 cites W2058800908 @default.
• W2107826964 cites W2062108627 @default.
• W2107826964 cites W2081299140 @default.
• W2107826964 cites W2092474188 @default.
• W2107826964 cites W2122496049 @default.
• W2107826964 cites W2134934522 @default.
• W2107826964 cites W2138355752 @default.
• W2107826964 cites W2153881449 @default.
• W2107826964 cites W292884782 @default.
• W2107826964 doi "https://doi.org/10.1093/nar/gnh023" @default.
• W2107826964 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/373419" @default.
• W2107826964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14960709" @default.
• W2107826964 hasPublicationYear "2004" @default.
• W2107826964 type Work @default.
• W2107826964 sameAs 2107826964 @default.
• W2107826964 citedByCount "20" @default.
• W2107826964 countsByYear W21078269642012 @default.
• W2107826964 countsByYear W21078269642014 @default.
• W2107826964 countsByYear W21078269642015 @default.
• W2107826964 countsByYear W21078269642018 @default.
• W2107826964 countsByYear W21078269642021 @default.
• W2107826964 crossrefType "journal-article" @default.
• W2107826964 hasAuthorship W2107826964A5030851843 @default.
• W2107826964 hasAuthorship W2107826964A5064912159 @default.
• W2107826964 hasBestOaLocation W21078269641 @default.
• W2107826964 hasConcept C102744134 @default.
• W2107826964 hasConcept C104317684 @default.
• W2107826964 hasConcept C111829913 @default.
• W2107826964 hasConcept C121050878 @default.
• W2107826964 hasConcept C134305767 @default.
• W2107826964 hasConcept C143065580 @default.
• W2107826964 hasConcept C153911025 @default.
• W2107826964 hasConcept C199360897 @default.
• W2107826964 hasConcept C202438428 @default.
• W2107826964 hasConcept C2778177303 @default.
• W2107826964 hasConcept C41008148 @default.
• W2107826964 hasConcept C49805395 @default.
• W2107826964 hasConcept C54355233 @default.
• W2107826964 hasConcept C78179603 @default.
• W2107826964 hasConcept C84597430 @default.
• W2107826964 hasConcept C86803240 @default.
• W2107826964 hasConcept C87073359 @default.
• W2107826964 hasConcept C95444343 @default.
• W2107826964 hasConceptScore W2107826964C102744134 @default.
• W2107826964 hasConceptScore W2107826964C104317684 @default.
• W2107826964 hasConceptScore W2107826964C111829913 @default.
• W2107826964 hasConceptScore W2107826964C121050878 @default.
• W2107826964 hasConceptScore W2107826964C134305767 @default.
• W2107826964 hasConceptScore W2107826964C143065580 @default.
• W2107826964 hasConceptScore W2107826964C153911025 @default.
• W2107826964 hasConceptScore W2107826964C199360897 @default.
• W2107826964 hasConceptScore W2107826964C202438428 @default.
• W2107826964 hasConceptScore W2107826964C2778177303 @default.
• W2107826964 hasConceptScore W2107826964C41008148 @default.
• W2107826964 hasConceptScore W2107826964C49805395 @default.
• W2107826964 hasConceptScore W2107826964C54355233 @default.
• W2107826964 hasConceptScore W2107826964C78179603 @default.
• W2107826964 hasConceptScore W2107826964C84597430 @default.
• W2107826964 hasConceptScore W2107826964C86803240 @default.
• W2107826964 hasConceptScore W2107826964C87073359 @default.
• W2107826964 hasConceptScore W2107826964C95444343 @default.
• W2107826964 hasIssue "3" @default.
• W2107826964 hasLocation W21078269641 @default.
• W2107826964 hasLocation W21078269642 @default.
• W2107826964 hasLocation W21078269643 @default.
• W2107826964 hasLocation W21078269644 @default.
• W2107826964 hasOpenAccess W2107826964 @default.
• W2107826964 hasPrimaryLocation W21078269641 @default.
• W2107826964 hasRelatedWork W15003116 @default.
• W2107826964 hasRelatedWork W1503592731 @default.
• W2107826964 hasRelatedWork W2073803229 @default.
• W2107826964 hasRelatedWork W2081299140 @default.
• W2107826964 hasRelatedWork W2107826964 @default.
• W2107826964 hasRelatedWork W2359584604 @default.
• W2107826964 hasRelatedWork W2394198949 @default.

• next