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- W2107883924 abstract "Oxidative phosphorylation (OXPHOS), the intracellular process that generates the majority of the ATP of a cell through the electron-transfer chain, is highly dependent on proteins encoded by the mitochondrial genome (mtDNA). MtDNA replication is regulated by the nuclear-encoded mitochondrial transcription factor A (TFAM) and the mitochondrial-specific DNA polymerase gamma, which consists of a catalytic (POLG) and an accessory (POLG2) subunit. Differentiation of pluripotent embryonic stem cells (ESCs) into specific cell types requires expansion of discrete populations of mitochondria and mtDNA replication to meet the specific metabolic requirements of the cell. We determined by real-time PCR that expression of pluripotent markers is reduced before the upregulation of Polg, Polg2 and Tfam in spontaneously differentiating R1 murine (m)ESCs, along with transient increases in mtDNA copy number. In D3 mESCs, the initial transient increase did not take place. However, precursors of neuronal and cardiomyocyte differentiation were positive for both POLG and TFAM. Similar-stage ESCs also showed active mtDNA replication, identified by 5-bromo-2′-deoxy-uridine labelling, as mtDNA copy number increased. Retinoic-acid-induced differentiation resulted in more consistent patterns of replication and upregulation of Polg, Polg2 and Tfam, whereas siRNA knockdown demonstrated that steady-state expression of POLG is essential for maintaining pluripotency." @default.
- W2107883924 created "2016-06-24" @default.
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- W2107883924 date "2007-11-15" @default.
- W2107883924 modified "2023-10-18" @default.
- W2107883924 title "Mitochondrial DNA replication during differentiation of murine embryonic stem cells" @default.
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- W2107883924 cites W183349502 @default.
- W2107883924 cites W1869370853 @default.
- W2107883924 cites W1945742482 @default.
- W2107883924 cites W1966697925 @default.
- W2107883924 cites W1968197570 @default.
- W2107883924 cites W1978422652 @default.
- W2107883924 cites W1980699340 @default.
- W2107883924 cites W1982555416 @default.
- W2107883924 cites W1986500594 @default.
- W2107883924 cites W1988888878 @default.
- W2107883924 cites W1992924696 @default.
- W2107883924 cites W1995140011 @default.
- W2107883924 cites W1996052541 @default.
- W2107883924 cites W1998826666 @default.
- W2107883924 cites W1999468613 @default.
- W2107883924 cites W2001256046 @default.
- W2107883924 cites W2006499356 @default.
- W2107883924 cites W2011073385 @default.
- W2107883924 cites W2012206386 @default.
- W2107883924 cites W2021494076 @default.
- W2107883924 cites W2025135710 @default.
- W2107883924 cites W2027635031 @default.
- W2107883924 cites W2032631439 @default.
- W2107883924 cites W2033871168 @default.
- W2107883924 cites W2036786006 @default.
- W2107883924 cites W2044494908 @default.
- W2107883924 cites W2044918998 @default.
- W2107883924 cites W2045388302 @default.
- W2107883924 cites W2045440019 @default.
- W2107883924 cites W2051070943 @default.
- W2107883924 cites W2052319461 @default.
- W2107883924 cites W2052801016 @default.
- W2107883924 cites W2053702504 @default.
- W2107883924 cites W2057973028 @default.
- W2107883924 cites W2061185374 @default.
- W2107883924 cites W2063873651 @default.
- W2107883924 cites W2065796189 @default.
- W2107883924 cites W2067322059 @default.
- W2107883924 cites W2070392246 @default.
- W2107883924 cites W2083237272 @default.
- W2107883924 cites W2083748438 @default.
- W2107883924 cites W2086087446 @default.
- W2107883924 cites W2086943348 @default.
- W2107883924 cites W2087301458 @default.
- W2107883924 cites W2091412371 @default.
- W2107883924 cites W2098246822 @default.
- W2107883924 cites W2103992993 @default.
- W2107883924 cites W2107273340 @default.
- W2107883924 cites W2108244474 @default.
- W2107883924 cites W2109291482 @default.
- W2107883924 cites W2116695601 @default.
- W2107883924 cites W2118397007 @default.
- W2107883924 cites W2118983368 @default.
- W2107883924 cites W2127367768 @default.
- W2107883924 cites W2132197657 @default.
- W2107883924 cites W2135668873 @default.
- W2107883924 cites W2141128578 @default.
- W2107883924 cites W2143146123 @default.
- W2107883924 cites W2154802086 @default.
- W2107883924 cites W2159047813 @default.
- W2107883924 cites W2160768847 @default.
- W2107883924 cites W2163176001 @default.
- W2107883924 cites W2922395101 @default.
- W2107883924 cites W4244429465 @default.
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- W2107883924 doi "https://doi.org/10.1242/jcs.016972" @default.
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