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- W2107890609 abstract "Importin α is the nuclear localization signal (NLS) receptor that is involved in the nuclear import of proteins containing basic NLSs. Using importin α as a tool, we were interested in determining whether the cytoskeleton could function in the transport of NLS-containing proteins from the cytoplasm to the nucleus. Double-labeling immunofluorescence studies showed that most of the cytoplasmic importin α coaligned with microtubules and microfilaments in tobacco protoplasts. Treatment of tobacco protoplasts with microtubule- or microfilament-depolymerizing agents disrupted the strands of importin α in the cytoplasm, whereas a microtubule-stabilizing agent had no effect. Biochemical analysis showed that importin α associated with microtubules and microfilaments in vitro in an NLS-dependent manner. The interaction of importin α with the cytoskeleton could be an essential element of protein transport from the cytoplasm to the nucleus in vivo." @default.
- W2107890609 created "2016-06-24" @default.
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- W2107890609 date "1998-11-01" @default.
- W2107890609 modified "2023-10-16" @default.
- W2107890609 title "Nuclear Localization Signal Receptor Importin α Associates with the Cytoskeleton" @default.
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- W2107890609 doi "https://doi.org/10.1105/tpc.10.11.1791" @default.
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