FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108016865> ?p ?o ?g. }

• W2108016865 endingPage "453" @default.
• W2108016865 startingPage "448" @default.
• W2108016865 abstract "Universal primers targeting conserved sequences flanking the 3' end of the 16S and the 5' end of the 23S rRNA genes (rDNAs) were used to amplify the 16S-23S rDNA internal transcribed spacers (ITS) from eight species of pseudomonads which have been associated with human infections. Amplicons from reference strains of Pseudomonas aeruginosa, Pseudomonas cepacia, Pseudomonas gladioli, Pseudomonas mallei, Pseudomonas mendocina, Pseudomonas pickettii, Pseudomonas pseudomallei, and Xanthomonas maltophilia were cloned from each species, and sequence analysis revealed a total of 19 distinct ITS regions, each defining a unique sequevar with ITS sizes ranging from 394 (P. cepacia) to 641 (P. pseudomallei) bp. Five distinct ITS sequevars in P. cepacia, four in P. mendocina, three in P. aeruginosa, two each in P. gladioli and P. pseudomallei, and one each in P. mallei, P. pickettii, and X. maltophilia were identified. With the exception of one P. cepacia ITS, all ITS regions contained potential tRNA sequences for isoleucine and/or alanine. On the basis of these ITS sequence data, species-specific oligonucleotide primers were designed to differentiate P. aeruginosa, P. cepacia, and P. pickettii. The specificities of these primers were investigated by testing 220 clinical isolates, including 101 strains of P. aeruginosa, 103 strains of P. cepacia, and 16 strains of P. pickettii, in addition to 24 American Type Culture Collection (ATCC) Pseudomonas strains. The results showed that single primer pairs directed at particular ITSs were capable of specifically identifying the ATCC reference strains and all of the clinical isolates of P. aeruginosa and P. pickettii, but this was not the case with several ITS-based primer pairs tested for P. cepacia. This pathogen, on the other hand, could be specifically identified by primer pairs directed against the 23S rDNA." @default.
• W2108016865 created "2016-06-24" @default.
• W2108016865 creator A5006259485 @default.
• W2108016865 creator A5024381943 @default.
• W2108016865 creator A5057470948 @default.
• W2108016865 creator A5064045418 @default.
• W2108016865 date "1995-07-01" @default.
• W2108016865 modified "2023-10-12" @default.
• W2108016865 title "Oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16S-23S rRNA internal transcribed spacers" @default.
• W2108016865 cites W1716783468 @default.
• W2108016865 cites W1754799330 @default.
• W2108016865 cites W1780620559 @default.
• W2108016865 cites W1877239098 @default.
• W2108016865 cites W1908540868 @default.
• W2108016865 cites W1952076072 @default.
• W2108016865 cites W1960080934 @default.
• W2108016865 cites W1968435596 @default.
• W2108016865 cites W2040628751 @default.
• W2108016865 cites W2057430453 @default.
• W2108016865 cites W2095175839 @default.
• W2108016865 cites W2096827106 @default.
• W2108016865 cites W2106722662 @default.
• W2108016865 cites W2125777028 @default.
• W2108016865 cites W2127405675 @default.
• W2108016865 cites W2146018009 @default.
• W2108016865 cites W2160004892 @default.
• W2108016865 cites W2328279504 @default.
• W2108016865 cites W276370537 @default.
• W2108016865 cites W4249569496 @default.
• W2108016865 doi "https://doi.org/10.1128/cdli.2.4.448-453.1995" @default.
• W2108016865 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/170177" @default.
• W2108016865 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7583922" @default.
• W2108016865 hasPublicationYear "1995" @default.
• W2108016865 type Work @default.
• W2108016865 sameAs 2108016865 @default.
• W2108016865 citedByCount "64" @default.
• W2108016865 countsByYear W21080168652012 @default.
• W2108016865 countsByYear W21080168652013 @default.
• W2108016865 countsByYear W21080168652014 @default.
• W2108016865 countsByYear W21080168652015 @default.
• W2108016865 countsByYear W21080168652016 @default.
• W2108016865 countsByYear W21080168652017 @default.
• W2108016865 countsByYear W21080168652021 @default.
• W2108016865 countsByYear W21080168652022 @default.
• W2108016865 crossrefType "journal-article" @default.
• W2108016865 hasAuthorship W2108016865A5006259485 @default.
• W2108016865 hasAuthorship W2108016865A5024381943 @default.
• W2108016865 hasAuthorship W2108016865A5057470948 @default.
• W2108016865 hasAuthorship W2108016865A5064045418 @default.
• W2108016865 hasBestOaLocation W21080168652 @default.
• W2108016865 hasConcept C104317684 @default.
• W2108016865 hasConcept C2777637488 @default.
• W2108016865 hasConcept C2781463711 @default.
• W2108016865 hasConcept C40749294 @default.
• W2108016865 hasConcept C49105822 @default.
• W2108016865 hasConcept C523546767 @default.
• W2108016865 hasConcept C54355233 @default.
• W2108016865 hasConcept C67705224 @default.
• W2108016865 hasConcept C67905577 @default.
• W2108016865 hasConcept C8185291 @default.
• W2108016865 hasConcept C86803240 @default.
• W2108016865 hasConcept C88478588 @default.
• W2108016865 hasConcept C89423630 @default.
• W2108016865 hasConceptScore W2108016865C104317684 @default.
• W2108016865 hasConceptScore W2108016865C2777637488 @default.
• W2108016865 hasConceptScore W2108016865C2781463711 @default.
• W2108016865 hasConceptScore W2108016865C40749294 @default.
• W2108016865 hasConceptScore W2108016865C49105822 @default.
• W2108016865 hasConceptScore W2108016865C523546767 @default.
• W2108016865 hasConceptScore W2108016865C54355233 @default.
• W2108016865 hasConceptScore W2108016865C67705224 @default.
• W2108016865 hasConceptScore W2108016865C67905577 @default.
• W2108016865 hasConceptScore W2108016865C8185291 @default.
• W2108016865 hasConceptScore W2108016865C86803240 @default.
• W2108016865 hasConceptScore W2108016865C88478588 @default.
• W2108016865 hasConceptScore W2108016865C89423630 @default.
• W2108016865 hasIssue "4" @default.
• W2108016865 hasLocation W21080168651 @default.
• W2108016865 hasLocation W21080168652 @default.
• W2108016865 hasLocation W21080168653 @default.
• W2108016865 hasOpenAccess W2108016865 @default.
• W2108016865 hasPrimaryLocation W21080168651 @default.
• W2108016865 hasRelatedWork W1698086670 @default.
• W2108016865 hasRelatedWork W1969290582 @default.
• W2108016865 hasRelatedWork W1973109513 @default.
• W2108016865 hasRelatedWork W2067833174 @default.
• W2108016865 hasRelatedWork W2068880248 @default.
• W2108016865 hasRelatedWork W2127112464 @default.
• W2108016865 hasRelatedWork W2143915336 @default.
• W2108016865 hasRelatedWork W2162814068 @default.
• W2108016865 hasRelatedWork W2409596141 @default.
• W2108016865 hasRelatedWork W25410580 @default.
• W2108016865 hasVolume "2" @default.
• W2108016865 isParatext "false" @default.
• W2108016865 isRetracted "false" @default.
• W2108016865 magId "2108016865" @default.
• W2108016865 workType "article" @default.