FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108138064> ?p ?o ?g. }

• W2108138064 endingPage "884" @default.
• W2108138064 startingPage "879" @default.
• W2108138064 abstract "ARM-seq enables enhanced sequencing of modified tRNAs and tRNA fragments. Treatment of RNA with the demethylase AlkB prior to reverse transcription removes three ‘hard-stop’ modifications, allowing for discovery of modified tRNA fragments and their precursors by RNA sequencing. High-throughput RNA sequencing has accelerated discovery of the complex regulatory roles of small RNAs, but RNAs containing modified nucleosides may escape detection when those modifications interfere with reverse transcription during RNA-seq library preparation. Here we describe AlkB-facilitated RNA methylation sequencing (ARM-seq), which uses pretreatment with Escherichia coli AlkB to demethylate N1-methyladenosine (m1A), N3-methylcytidine (m3C) and N1-methylguanosine (m1G), all commonly found in tRNAs. Comparative methylation analysis using ARM-seq provides the first detailed, transcriptome-scale map of these modifications and reveals an abundance of previously undetected, methylated small RNAs derived from tRNAs. ARM-seq demonstrates that tRNA fragments accurately recapitulate the m1A modification state for well-characterized yeast tRNAs and generates new predictions for a large number of human tRNAs, including tRNA precursors and mitochondrial tRNAs. Thus, ARM-seq provides broad utility for identifying previously overlooked methyl-modified RNAs, can efficiently monitor methylation state and may reveal new roles for tRNA fragments as biomarkers or signaling molecules." @default.
• W2108138064 created "2016-06-24" @default.
• W2108138064 creator A5011365621 @default.
• W2108138064 creator A5016860853 @default.
• W2108138064 creator A5029891285 @default.
• W2108138064 creator A5059159279 @default.
• W2108138064 creator A5080580237 @default.
• W2108138064 creator A5081624140 @default.
• W2108138064 date "2015-08-03" @default.
• W2108138064 modified "2023-10-16" @default.
• W2108138064 title "ARM-seq: AlkB-facilitated RNA methylation sequencing reveals a complex landscape of modified tRNA fragments" @default.
• W2108138064 cites W1187995737 @default.
• W2108138064 cites W1562507949 @default.
• W2108138064 cites W1566729113 @default.
• W2108138064 cites W1982749428 @default.
• W2108138064 cites W1983255679 @default.
• W2108138064 cites W1983859619 @default.
• W2108138064 cites W2007801600 @default.
• W2108138064 cites W2021039243 @default.
• W2108138064 cites W2021599506 @default.
• W2108138064 cites W2022465064 @default.
• W2108138064 cites W2022862766 @default.
• W2108138064 cites W2036649644 @default.
• W2108138064 cites W2055101024 @default.
• W2108138064 cites W2058767191 @default.
• W2108138064 cites W2060902122 @default.
• W2108138064 cites W2065129911 @default.
• W2108138064 cites W2065847836 @default.
• W2108138064 cites W2067022150 @default.
• W2108138064 cites W2076772609 @default.
• W2108138064 cites W2085928743 @default.
• W2108138064 cites W2093956979 @default.
• W2108138064 cites W2097843864 @default.
• W2108138064 cites W2106039274 @default.
• W2108138064 cites W2107400254 @default.
• W2108138064 cites W2112192177 @default.
• W2108138064 cites W2112765717 @default.
• W2108138064 cites W2113263393 @default.
• W2108138064 cites W2117567932 @default.
• W2108138064 cites W2119264359 @default.
• W2108138064 cites W2119635233 @default.
• W2108138064 cites W2136270995 @default.
• W2108138064 cites W2145067337 @default.
• W2108138064 cites W2148224382 @default.
• W2108138064 cites W2149429041 @default.
• W2108138064 cites W2152114580 @default.
• W2108138064 cites W2158472340 @default.
• W2108138064 cites W2160475169 @default.
• W2108138064 cites W2161890613 @default.
• W2108138064 cites W2164256634 @default.
• W2108138064 cites W2170551349 @default.
• W2108138064 cites W2179438025 @default.
• W2108138064 doi "https://doi.org/10.1038/nmeth.3508" @default.
• W2108138064 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4553111" @default.
• W2108138064 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26237225" @default.
• W2108138064 hasPublicationYear "2015" @default.
• W2108138064 type Work @default.
• W2108138064 sameAs 2108138064 @default.
• W2108138064 citedByCount "311" @default.
• W2108138064 countsByYear W21081380642015 @default.
• W2108138064 countsByYear W21081380642016 @default.
• W2108138064 countsByYear W21081380642017 @default.
• W2108138064 countsByYear W21081380642018 @default.
• W2108138064 countsByYear W21081380642019 @default.
• W2108138064 countsByYear W21081380642020 @default.
• W2108138064 countsByYear W21081380642021 @default.
• W2108138064 countsByYear W21081380642022 @default.
• W2108138064 countsByYear W21081380642023 @default.
• W2108138064 crossrefType "journal-article" @default.
• W2108138064 hasAuthorship W2108138064A5011365621 @default.
• W2108138064 hasAuthorship W2108138064A5016860853 @default.
• W2108138064 hasAuthorship W2108138064A5029891285 @default.
• W2108138064 hasAuthorship W2108138064A5059159279 @default.
• W2108138064 hasAuthorship W2108138064A5080580237 @default.
• W2108138064 hasAuthorship W2108138064A5081624140 @default.
• W2108138064 hasBestOaLocation W21081380642 @default.
• W2108138064 hasConcept C104317684 @default.
• W2108138064 hasConcept C132917006 @default.
• W2108138064 hasConcept C141231307 @default.
• W2108138064 hasConcept C150194340 @default.
• W2108138064 hasConcept C153957851 @default.
• W2108138064 hasConcept C162317418 @default.
• W2108138064 hasConcept C2777721518 @default.
• W2108138064 hasConcept C2909856833 @default.
• W2108138064 hasConcept C33288867 @default.
• W2108138064 hasConcept C54355233 @default.
• W2108138064 hasConcept C547475151 @default.
• W2108138064 hasConcept C67705224 @default.
• W2108138064 hasConcept C70721500 @default.
• W2108138064 hasConcept C86803240 @default.
• W2108138064 hasConcept C91965660 @default.
• W2108138064 hasConceptScore W2108138064C104317684 @default.
• W2108138064 hasConceptScore W2108138064C132917006 @default.
• W2108138064 hasConceptScore W2108138064C141231307 @default.
• W2108138064 hasConceptScore W2108138064C150194340 @default.
• W2108138064 hasConceptScore W2108138064C153957851 @default.
• W2108138064 hasConceptScore W2108138064C162317418 @default.
• W2108138064 hasConceptScore W2108138064C2777721518 @default.

• next