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• W2108149119 startingPage "876" @default.
• W2108149119 abstract "SUMMARY Eimeria maxima has been used as a model apicomplexan parasite to study sexual stage development and oocyst wall formation. A complete understanding of the wall's biochemical and biophysical properties is of great interest in research on all apicomplexan parasites. Purified gametocytes, zygotes and oocysts were analysed by three-dimensional confocal microscopy, and wide-field fluorescent microscopy was used to investigate the appearance and spatial organization of the 2 types of wall-forming bodies (WFBs). In addition, a variety of staining procedures and immunoassays were used to assess the biosynthesis, metabolic activity, intactness and molecular composition of the WFBs in situ . WFBs were extracted from gametocytes/zygotes and their composition was assessed by microscopy and SDS-PAGE analysis. It was concluded that isolated gametocytes are intact and metabolically active. Additionally, it was observed that the Type 1 WFBs are aligned at the periphery of the parasite and fuse together producing neutral lipid rich patches that appear to be inserted into the space between 2 parasite-specific membranes. Finally, it was shown that the WFBs extracted from purified gametocytes had the same shape, size and staining properties as those observed in situ , and contained the major glycoprotein antigens known to be present in these organelles." @default.
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• W2108149119 date "2013-03-14" @default.
• W2108149119 modified "2023-09-23" @default.
• W2108149119 title "The spatial organization and extraction of the wall-forming bodies of<i>Eimeria maxima</i>" @default.
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• W2108149119 doi "https://doi.org/10.1017/s0031182012002247" @default.
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