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• W2108151320 abstract "DnaA plays a central role in initiation of DNA replication at oriC in bacteria, and is also a transcription regulator which interacts with the DnaA box relative to a specific gene. Through screening the interaction between TtDnaA and the transcription machinery in Thermoanaerobacter tengcongensis by yeast two-hybrid assays, we found for the first time that the TtDnaA could interact with an anti-terminator, TtNusG2, in this thermophilic bacterium. The direct interaction between TtDnaA and TtNusG2 was verified by surface plasmon resonance (SPR) assay in vitro, and was further confirmed by co-immunoprecipitation assay in vivo. Moreover, we demonstrated that domain I and domain III of TtDnaA were responsible for the interaction with TtNusG2. These findings might expand our understanding of cooperation of two fundamental processes, replication and transcription, in this bacterium." @default.
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• W2108151320 date "2008-07-01" @default.
• W2108151320 modified "2023-10-17" @default.
• W2108151320 title "Replication initiator DnaA interacts with an anti-terminator NusG in T. tengcongensis" @default.
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• W2108151320 doi "https://doi.org/10.1016/j.bbrc.2008.04.131" @default.
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