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- W2108161018 abstract "This study sought to evaluate a new combined gene and protein detection platform in the context of HER2 evaluation in breast and gastric carcinomas. HER2 immunohistochemistry (IHC) and dual color in situ hybridization (Dual ISH) were combined on a single slide. Results were compared with conventional HER2 IHC and fluorescence ISH. Results from the gene and protein assay were reliable and highly reproducible for both breast and gastric carcinomas. Concordance was found between conventional HER2 IHC and ISH testing and the gene and protein assay in the same laboratory (>95% for Dual ISH; lower for IHC because of different antibody clones), between IHC and Dual ISH performed on the same slide (>92%), and in the gene and protein assays between laboratories (>96%). This cost- and time-effective method provides fast and definitive results (IHC confirmed by means of Dual ISH) to aid in rapid treatment decisions. It can also be applied to other gene and protein combinations." @default.
- W2108161018 created "2016-06-24" @default.
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- W2108161018 date "2012-12-01" @default.
- W2108161018 modified "2023-09-26" @default.
- W2108161018 title "Simultaneous Analysis of HER2 Gene and Protein on a Single Slide Facilitates HER2 Testing of Breast and Gastric Carcinomas" @default.
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- W2108161018 doi "https://doi.org/10.1309/ajcpl5iv0lawserg" @default.
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