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• W2108270955 abstract "Tumor antigen-specific T cell clones represent a useful tool in tumor immunology; however, their long-term culture is limited. To generate an immortalized cytotoxic T cell clone against the human tumor antigen mucin, we exposed a previously generated T cell culture to Herpesvirus saimiri. We obtained an immortalized human CD4+ T cell clone, termed SITAM. Clonality of these cells was shown by analysis of the alpha/beta-T cell receptor (TCR) repertoire. Cytolytic activity was demonstrated against several mucin-expressing tumor cell lines and could not be detected against non-mucin-expressing cells. SITAM cells maintained their features stably for 2 years. Furthermore, growth of the tumor cell line Capan-2 in NOD/SCID mice was inhibited when SITAM cells were coinjected subcutaneously with tumor cells. SITAM cells provide an unlimited source of clonal T cells for analysis of tumor recognition and may be of help in TCR-targeted immunotherapy." @default.
• W2108270955 created "2016-06-24" @default.
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• W2108270955 date "2001-05-01" @default.
• W2108270955 modified "2023-10-01" @default.
• W2108270955 title "Generation of an Immortalized Human CD4+ T Cell Clone Inhibiting Tumor Growth in Mice" @default.
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• W2108270955 doi "https://doi.org/10.1006/bbrc.2001.4846" @default.
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