Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108318932> ?p ?o ?g. }
- W2108318932 endingPage "714" @default.
- W2108318932 startingPage "708" @default.
- W2108318932 abstract "Abstract Recombinant consensus interferon (CIFN) is a therapeutic protein with molecular weight of 19.5 kDa having broad spectrum antiviral activity. Recombinant human CIFN (rhCIFN) has previously been expressed in Escherichia coli using isopropyl‐β‐ d ‐thiogalactopyranoside (IPTG), a non‐metabolizable and expensive compound, as inducer. For economical and commercial‐scale recombinant protein production, it is greatly needed to increase the product yield in a limited time frame to reduce the processing cost. To reduce the cost of production of rhCIFN in E. coli , induction was accomplished by using lactose instead of IPTG. Lactose induction (14 g/L) in shake flask experiment resulted in higher yield as compared with 1 mM IPTG. Finally, with single‐step purification on DEAE sepharose, 150 mg/L of >98% pure rhCIFN was achieved. In the present study, an attempt was made to develop a low cost process for producing quality product with high purity. Methods devised may be helpful for pilot‐scale production of recombinant proteins at low cost." @default.
- W2108318932 created "2016-06-24" @default.
- W2108318932 creator A5005999074 @default.
- W2108318932 creator A5040754239 @default.
- W2108318932 creator A5050440812 @default.
- W2108318932 creator A5066870764 @default.
- W2108318932 creator A5066873531 @default.
- W2108318932 creator A5069992127 @default.
- W2108318932 creator A5074907594 @default.
- W2108318932 creator A5080202547 @default.
- W2108318932 date "2015-10-11" @default.
- W2108318932 modified "2023-10-11" @default.
- W2108318932 title "Simple procedure applying lactose induction and one-step purification for high-yield production of rhCIFN" @default.
- W2108318932 cites W1784952030 @default.
- W2108318932 cites W1968089709 @default.
- W2108318932 cites W1984119456 @default.
- W2108318932 cites W2002746052 @default.
- W2108318932 cites W2023607480 @default.
- W2108318932 cites W2028816545 @default.
- W2108318932 cites W2043886637 @default.
- W2108318932 cites W2063313184 @default.
- W2108318932 cites W2064890428 @default.
- W2108318932 cites W2068411237 @default.
- W2108318932 cites W2084321999 @default.
- W2108318932 cites W2098346284 @default.
- W2108318932 cites W2100837269 @default.
- W2108318932 cites W2113653720 @default.
- W2108318932 cites W2129217817 @default.
- W2108318932 cites W2134226107 @default.
- W2108318932 cites W2143481612 @default.
- W2108318932 cites W2145250917 @default.
- W2108318932 cites W2150996203 @default.
- W2108318932 cites W2177943720 @default.
- W2108318932 cites W2333515303 @default.
- W2108318932 cites W2782303467 @default.
- W2108318932 cites W3024136902 @default.
- W2108318932 cites W4293247451 @default.
- W2108318932 doi "https://doi.org/10.1002/bab.1426" @default.
- W2108318932 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26256695" @default.
- W2108318932 hasPublicationYear "2015" @default.
- W2108318932 type Work @default.
- W2108318932 sameAs 2108318932 @default.
- W2108318932 citedByCount "15" @default.
- W2108318932 countsByYear W21083189322016 @default.
- W2108318932 countsByYear W21083189322017 @default.
- W2108318932 countsByYear W21083189322019 @default.
- W2108318932 countsByYear W21083189322020 @default.
- W2108318932 countsByYear W21083189322021 @default.
- W2108318932 countsByYear W21083189322022 @default.
- W2108318932 countsByYear W21083189322023 @default.
- W2108318932 crossrefType "journal-article" @default.
- W2108318932 hasAuthorship W2108318932A5005999074 @default.
- W2108318932 hasAuthorship W2108318932A5040754239 @default.
- W2108318932 hasAuthorship W2108318932A5050440812 @default.
- W2108318932 hasAuthorship W2108318932A5066870764 @default.
- W2108318932 hasAuthorship W2108318932A5066873531 @default.
- W2108318932 hasAuthorship W2108318932A5069992127 @default.
- W2108318932 hasAuthorship W2108318932A5074907594 @default.
- W2108318932 hasAuthorship W2108318932A5080202547 @default.
- W2108318932 hasConcept C104317684 @default.
- W2108318932 hasConcept C127413603 @default.
- W2108318932 hasConcept C134121241 @default.
- W2108318932 hasConcept C170344550 @default.
- W2108318932 hasConcept C185592680 @default.
- W2108318932 hasConcept C191897082 @default.
- W2108318932 hasConcept C192562407 @default.
- W2108318932 hasConcept C2225953 @default.
- W2108318932 hasConcept C2778724459 @default.
- W2108318932 hasConcept C2985847231 @default.
- W2108318932 hasConcept C40767141 @default.
- W2108318932 hasConcept C43617362 @default.
- W2108318932 hasConcept C547475151 @default.
- W2108318932 hasConcept C55493867 @default.
- W2108318932 hasConcept C78519656 @default.
- W2108318932 hasConceptScore W2108318932C104317684 @default.
- W2108318932 hasConceptScore W2108318932C127413603 @default.
- W2108318932 hasConceptScore W2108318932C134121241 @default.
- W2108318932 hasConceptScore W2108318932C170344550 @default.
- W2108318932 hasConceptScore W2108318932C185592680 @default.
- W2108318932 hasConceptScore W2108318932C191897082 @default.
- W2108318932 hasConceptScore W2108318932C192562407 @default.
- W2108318932 hasConceptScore W2108318932C2225953 @default.
- W2108318932 hasConceptScore W2108318932C2778724459 @default.
- W2108318932 hasConceptScore W2108318932C2985847231 @default.
- W2108318932 hasConceptScore W2108318932C40767141 @default.
- W2108318932 hasConceptScore W2108318932C43617362 @default.
- W2108318932 hasConceptScore W2108318932C547475151 @default.
- W2108318932 hasConceptScore W2108318932C55493867 @default.
- W2108318932 hasConceptScore W2108318932C78519656 @default.
- W2108318932 hasIssue "5" @default.
- W2108318932 hasLocation W21083189321 @default.
- W2108318932 hasLocation W21083189322 @default.
- W2108318932 hasOpenAccess W2108318932 @default.
- W2108318932 hasPrimaryLocation W21083189321 @default.
- W2108318932 hasRelatedWork W1588856937 @default.
- W2108318932 hasRelatedWork W2037323891 @default.
- W2108318932 hasRelatedWork W2352852219 @default.
- W2108318932 hasRelatedWork W2356506558 @default.