FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108346683> ?p ?o ?g. }

• W2108346683 endingPage "919" @default.
• W2108346683 startingPage "911" @default.
• W2108346683 abstract "Garvicin ML (GarML) is a circular bacteriocin produced by Lactococcus garvieae DCC43. The recently published draft genome of this strain allowed determination of the genetic background for bacteriocin production. Bioinformatic analysis identified a gene cluster consisting of nine open reading frames likely involved in the production of and immunity to GarML. The garA gene encodes the bacteriocin precursor, garX a large transmembrane protein, garBCDE a putative immunity protein (garB) followed by an ATPase and two transmembrane proteins, and garFGH a putative ABC transporter complex. Functional genetic analysis revealed that deletion of garFGH had no effect on sensitivity to or production of GarML. In contrast, deletion of garBCDE or inactivation of garX resulted in high-level sensitivity to GarML and completely abolished production of active bacteriocin. Mass spectrometry of culture supernatants revealed that wild-type cultures contained the mature circular form as well as the linear forms of the bacteriocin, both with and without the three-amino-acid leader sequence, while bacteriocin-negative mutants contained only the linear forms. These results indicate that cleavage of the leader peptide precedes circularization and is likely performed by a functional entity separate from the GarML gene cluster. To our knowledge, this is the first conclusive evidence for these processes being separated in time. Loss of immunity and antimicrobial activity in addition to our inability to detect the circular bacteriocin in the ΔgarBCDE and garX::pCG47 mutants demonstrate that both these units are indispensable for GarML biosynthesis as well as immunity. Furthermore, the results indicate that these genes are implicated in the circularization of the bacteriocin and that their functions are probably interlinked." @default.
• W2108346683 created "2016-06-24" @default.
• W2108346683 creator A5010563845 @default.
• W2108346683 creator A5020537889 @default.
• W2108346683 creator A5061710272 @default.
• W2108346683 creator A5063386849 @default.
• W2108346683 creator A5091233106 @default.
• W2108346683 date "2013-12-13" @default.
• W2108346683 modified "2023-09-23" @default.
• W2108346683 title "Functional Genetic Analysis of the GarML Gene Cluster in Lactococcus garvieae DCC43 Gives New Insights into Circular Bacteriocin Biosynthesis" @default.
• W2108346683 cites W1835465021 @default.
• W2108346683 cites W1865024295 @default.
• W2108346683 cites W1902304336 @default.
• W2108346683 cites W1964338435 @default.
• W2108346683 cites W1964470616 @default.
• W2108346683 cites W1981813945 @default.
• W2108346683 cites W1988876705 @default.
• W2108346683 cites W1999652679 @default.
• W2108346683 cites W2003236418 @default.
• W2108346683 cites W2004418119 @default.
• W2108346683 cites W2004634628 @default.
• W2108346683 cites W2009221323 @default.
• W2108346683 cites W2009283988 @default.
• W2108346683 cites W2026245939 @default.
• W2108346683 cites W2028245576 @default.
• W2108346683 cites W2055043387 @default.
• W2108346683 cites W2056717825 @default.
• W2108346683 cites W2059576791 @default.
• W2108346683 cites W2060178110 @default.
• W2108346683 cites W2071422363 @default.
• W2108346683 cites W2073606320 @default.
• W2108346683 cites W2082059574 @default.
• W2108346683 cites W2089705909 @default.
• W2108346683 cites W2095393087 @default.
• W2108346683 cites W2098554849 @default.
• W2108346683 cites W2102298254 @default.
• W2108346683 cites W2108649941 @default.
• W2108346683 cites W2109276669 @default.
• W2108346683 cites W2110425545 @default.
• W2108346683 cites W2111931465 @default.
• W2108346683 cites W2114645323 @default.
• W2108346683 cites W2123119918 @default.
• W2108346683 cites W2139021858 @default.
• W2108346683 cites W2144362290 @default.
• W2108346683 cites W2147912681 @default.
• W2108346683 cites W2152301430 @default.
• W2108346683 cites W2156340561 @default.
• W2108346683 cites W2157939704 @default.
• W2108346683 cites W2158013238 @default.
• W2108346683 cites W2160462976 @default.
• W2108346683 cites W2162031862 @default.
• W2108346683 cites W2162792752 @default.
• W2108346683 cites W2162854463 @default.
• W2108346683 cites W2167410536 @default.
• W2108346683 cites W2167767378 @default.
• W2108346683 cites W2170566642 @default.
• W2108346683 cites W2783981376 @default.
• W2108346683 cites W4233351338 @default.
• W2108346683 doi "https://doi.org/10.1128/jb.01115-13" @default.
• W2108346683 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3957696" @default.
• W2108346683 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24336941" @default.
• W2108346683 hasPublicationYear "2013" @default.
• W2108346683 type Work @default.
• W2108346683 sameAs 2108346683 @default.
• W2108346683 citedByCount "19" @default.
• W2108346683 countsByYear W21083466832014 @default.
• W2108346683 countsByYear W21083466832015 @default.
• W2108346683 countsByYear W21083466832016 @default.
• W2108346683 countsByYear W21083466832017 @default.
• W2108346683 countsByYear W21083466832018 @default.
• W2108346683 countsByYear W21083466832020 @default.
• W2108346683 countsByYear W21083466832021 @default.
• W2108346683 countsByYear W21083466832023 @default.
• W2108346683 crossrefType "journal-article" @default.
• W2108346683 hasAuthorship W2108346683A5010563845 @default.
• W2108346683 hasAuthorship W2108346683A5020537889 @default.
• W2108346683 hasAuthorship W2108346683A5061710272 @default.
• W2108346683 hasAuthorship W2108346683A5063386849 @default.
• W2108346683 hasAuthorship W2108346683A5091233106 @default.
• W2108346683 hasBestOaLocation W21083466831 @default.
• W2108346683 hasConcept C104317684 @default.
• W2108346683 hasConcept C115574571 @default.
• W2108346683 hasConcept C132141352 @default.
• W2108346683 hasConcept C143065580 @default.
• W2108346683 hasConcept C36857842 @default.
• W2108346683 hasConcept C523546767 @default.
• W2108346683 hasConcept C54355233 @default.
• W2108346683 hasConcept C86803240 @default.
• W2108346683 hasConcept C89423630 @default.
• W2108346683 hasConceptScore W2108346683C104317684 @default.
• W2108346683 hasConceptScore W2108346683C115574571 @default.
• W2108346683 hasConceptScore W2108346683C132141352 @default.
• W2108346683 hasConceptScore W2108346683C143065580 @default.
• W2108346683 hasConceptScore W2108346683C36857842 @default.
• W2108346683 hasConceptScore W2108346683C523546767 @default.
• W2108346683 hasConceptScore W2108346683C54355233 @default.
• W2108346683 hasConceptScore W2108346683C86803240 @default.
• W2108346683 hasConceptScore W2108346683C89423630 @default.

• next