FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108355596> ?p ?o ?g. }

• W2108355596 endingPage "374" @default.
• W2108355596 startingPage "363" @default.
• W2108355596 abstract "Exoribonuclease II (RNase II), encoded by the rnb gene, is a ubiquitous enzyme that is responsible for 90% of the hydrolytic activity in Escherichia coli crude extracts. The E. coli strain SK4803, carrying the mutant allele rnb296, has been widely used in the study of the role of RNase II. We determined the DNA sequence of rnb296 and cloned this mutant gene in an expression vector. Only a point mutation in the coding sequence of the gene was detected, which results in the single substitution of aspartate 209 for asparagine. The mutant and the wild-type RNase II enzymes were purified, and their 3' to 5' exoribonucleolytic activity, as well as their RNA binding capability, were characterized. We also studied the metal dependency of the exoribonuclease activity of RNase II. The results obtained demonstrated that aspartate 209 is absolutely essential for RNA hydrolysis, but is not required for substrate binding. This is the first evidence of an acidic residue that is essential for the activity of RNase II-like enzymes. The possible involvement of this residue in metal binding at the active site of the enzyme is discussed. These results are particularly relevant at this time given that no structural or mutational analysis has been performed for any protein of the RNR family of exoribonucleases." @default.
• W2108355596 created "2016-06-24" @default.
• W2108355596 creator A5039556725 @default.
• W2108355596 creator A5069854122 @default.
• W2108355596 date "2004-12-21" @default.
• W2108355596 modified "2023-09-30" @default.
• W2108355596 title "A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding" @default.
• W2108355596 cites W1509760380 @default.
• W2108355596 cites W1525643420 @default.
• W2108355596 cites W1572002230 @default.
• W2108355596 cites W1699731869 @default.
• W2108355596 cites W1750148576 @default.
• W2108355596 cites W1775749144 @default.
• W2108355596 cites W1873199501 @default.
• W2108355596 cites W1975993749 @default.
• W2108355596 cites W1980163639 @default.
• W2108355596 cites W1988098435 @default.
• W2108355596 cites W1996031986 @default.
• W2108355596 cites W1999894502 @default.
• W2108355596 cites W2009429279 @default.
• W2108355596 cites W2014746960 @default.
• W2108355596 cites W2026430354 @default.
• W2108355596 cites W2028622989 @default.
• W2108355596 cites W2036290842 @default.
• W2108355596 cites W2045108614 @default.
• W2108355596 cites W2052200921 @default.
• W2108355596 cites W2053643897 @default.
• W2108355596 cites W2063335878 @default.
• W2108355596 cites W2079208013 @default.
• W2108355596 cites W2084371615 @default.
• W2108355596 cites W2084635213 @default.
• W2108355596 cites W2085776880 @default.
• W2108355596 cites W2093612041 @default.
• W2108355596 cites W2094001587 @default.
• W2108355596 cites W2103374070 @default.
• W2108355596 cites W2110460574 @default.
• W2108355596 cites W2122303498 @default.
• W2108355596 cites W2131114832 @default.
• W2108355596 cites W2133286749 @default.
• W2108355596 cites W2138524348 @default.
• W2108355596 cites W2144259553 @default.
• W2108355596 cites W2153637982 @default.
• W2108355596 cites W2153729611 @default.
• W2108355596 cites W2163043365 @default.
• W2108355596 cites W2164949415 @default.
• W2108355596 cites W2165080174 @default.
• W2108355596 cites W2167485146 @default.
• W2108355596 cites W2168539350 @default.
• W2108355596 cites W2170531284 @default.
• W2108355596 cites W2297876998 @default.
• W2108355596 cites W41568031 @default.
• W2108355596 doi "https://doi.org/10.1111/j.1742-4658.2004.04477.x" @default.
• W2108355596 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15654875" @default.
• W2108355596 hasPublicationYear "2004" @default.
• W2108355596 type Work @default.
• W2108355596 sameAs 2108355596 @default.
• W2108355596 citedByCount "45" @default.
• W2108355596 countsByYear W21083555962012 @default.
• W2108355596 countsByYear W21083555962013 @default.
• W2108355596 countsByYear W21083555962014 @default.
• W2108355596 countsByYear W21083555962019 @default.
• W2108355596 countsByYear W21083555962020 @default.
• W2108355596 countsByYear W21083555962022 @default.
• W2108355596 countsByYear W21083555962023 @default.
• W2108355596 crossrefType "journal-article" @default.
• W2108355596 hasAuthorship W2108355596A5039556725 @default.
• W2108355596 hasAuthorship W2108355596A5069854122 @default.
• W2108355596 hasConcept C104317684 @default.
• W2108355596 hasConcept C10879258 @default.
• W2108355596 hasConcept C143065580 @default.
• W2108355596 hasConcept C153911025 @default.
• W2108355596 hasConcept C181199279 @default.
• W2108355596 hasConcept C188528350 @default.
• W2108355596 hasConcept C195500695 @default.
• W2108355596 hasConcept C2775895851 @default.
• W2108355596 hasConcept C2775990537 @default.
• W2108355596 hasConcept C2776719180 @default.
• W2108355596 hasConcept C2778976237 @default.
• W2108355596 hasConcept C2780394083 @default.
• W2108355596 hasConcept C54355233 @default.
• W2108355596 hasConcept C547475151 @default.
• W2108355596 hasConcept C55493867 @default.
• W2108355596 hasConcept C67705224 @default.
• W2108355596 hasConcept C69766542 @default.
• W2108355596 hasConcept C86803240 @default.
• W2108355596 hasConceptScore W2108355596C104317684 @default.
• W2108355596 hasConceptScore W2108355596C10879258 @default.
• W2108355596 hasConceptScore W2108355596C143065580 @default.
• W2108355596 hasConceptScore W2108355596C153911025 @default.
• W2108355596 hasConceptScore W2108355596C181199279 @default.
• W2108355596 hasConceptScore W2108355596C188528350 @default.
• W2108355596 hasConceptScore W2108355596C195500695 @default.
• W2108355596 hasConceptScore W2108355596C2775895851 @default.
• W2108355596 hasConceptScore W2108355596C2775990537 @default.
• W2108355596 hasConceptScore W2108355596C2776719180 @default.
• W2108355596 hasConceptScore W2108355596C2778976237 @default.
• W2108355596 hasConceptScore W2108355596C2780394083 @default.
• W2108355596 hasConceptScore W2108355596C54355233 @default.

• next