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- W2108363963 abstract "The Nup107-160 complex is a critical subunit of the nuclear pore. This complex localizes to kinetochores in mitotic mammalian cells, where its function is unknown. To examine Nup107-160 complex recruitment to kinetochores, we stained human cells with antisera to four complex components. Each antibody stained not only kinetochores but also prometaphase spindle poles and proximal spindle fibers, mirroring the dual prometaphase localization of the spindle checkpoint proteins Mad1, Mad2, Bub3, and Cdc20. Indeed, expanded crescents of the Nup107-160 complex encircled unattached kinetochores, similar to the hyperaccumulation observed of dynamic outer kinetochore checkpoint proteins and motors at unattached kinetochores. In mitotic Xenopus egg extracts, the Nup107-160 complex localized throughout reconstituted spindles. When the Nup107-160 complex was depleted from extracts, the spindle checkpoint remained intact, but spindle assembly was rendered strikingly defective. Microtubule nucleation around sperm centrosomes seemed normal, but the microtubules quickly disassembled, leaving largely unattached sperm chromatin. Notably, Ran-GTP caused normal assembly of microtubule asters in depleted extracts, indicating that this defect was upstream of Ran or independent of it. We conclude that the Nup107-160 complex is dynamic in mitosis and that it promotes spindle assembly in a manner that is distinct from its functions at interphase nuclear pores." @default.
- W2108363963 created "2016-06-24" @default.
- W2108363963 creator A5012296563 @default.
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- W2108363963 creator A5089383987 @default.
- W2108363963 date "2006-09-01" @default.
- W2108363963 modified "2023-10-17" @default.
- W2108363963 title "The Nup107-160 Nucleoporin Complex Is Required for Correct Bipolar Spindle Assembly" @default.
- W2108363963 cites W149621205 @default.
- W2108363963 cites W1606409542 @default.
- W2108363963 cites W1735762627 @default.
- W2108363963 cites W1961562730 @default.
- W2108363963 cites W1964253746 @default.
- W2108363963 cites W1966139298 @default.
- W2108363963 cites W1966205440 @default.
- W2108363963 cites W1966723276 @default.
- W2108363963 cites W1966928239 @default.
- W2108363963 cites W1967890631 @default.
- W2108363963 cites W1978450158 @default.
- W2108363963 cites W1987530377 @default.
- W2108363963 cites W1988573682 @default.
- W2108363963 cites W1991690400 @default.
- W2108363963 cites W1994177513 @default.
- W2108363963 cites W1997284260 @default.
- W2108363963 cites W1998359653 @default.
- W2108363963 cites W1999081109 @default.
- W2108363963 cites W1999144095 @default.
- W2108363963 cites W2001887737 @default.
- W2108363963 cites W2007388359 @default.
- W2108363963 cites W2008985517 @default.
- W2108363963 cites W2009911474 @default.
- W2108363963 cites W2011052397 @default.
- W2108363963 cites W2015557154 @default.
- W2108363963 cites W2016706874 @default.
- W2108363963 cites W2016865656 @default.
- W2108363963 cites W2017513475 @default.
- W2108363963 cites W2022450237 @default.
- W2108363963 cites W2027766408 @default.
- W2108363963 cites W2029225125 @default.
- W2108363963 cites W2033062977 @default.
- W2108363963 cites W2036760739 @default.
- W2108363963 cites W2037624113 @default.
- W2108363963 cites W2044016151 @default.
- W2108363963 cites W2046115216 @default.
- W2108363963 cites W2047182427 @default.
- W2108363963 cites W2050908337 @default.
- W2108363963 cites W2053416801 @default.
- W2108363963 cites W2057123629 @default.
- W2108363963 cites W2061637725 @default.
- W2108363963 cites W2063761203 @default.
- W2108363963 cites W2064062498 @default.
- W2108363963 cites W2065600921 @default.
- W2108363963 cites W2066348389 @default.
- W2108363963 cites W2073526608 @default.
- W2108363963 cites W2074561346 @default.
- W2108363963 cites W2080010961 @default.
- W2108363963 cites W2080458584 @default.
- W2108363963 cites W2082936054 @default.
- W2108363963 cites W2085304899 @default.
- W2108363963 cites W2094533427 @default.
- W2108363963 cites W2095577003 @default.
- W2108363963 cites W2095744767 @default.
- W2108363963 cites W2101712721 @default.
- W2108363963 cites W2103656871 @default.
- W2108363963 cites W2104104972 @default.
- W2108363963 cites W2105488407 @default.
- W2108363963 cites W2109592565 @default.
- W2108363963 cites W2111939527 @default.
- W2108363963 cites W2114187223 @default.
- W2108363963 cites W2114918924 @default.
- W2108363963 cites W2133695223 @default.
- W2108363963 cites W2136931082 @default.
- W2108363963 cites W2140235777 @default.
- W2108363963 cites W2140463144 @default.
- W2108363963 cites W2142053904 @default.
- W2108363963 cites W2144245358 @default.
- W2108363963 cites W2145131593 @default.
- W2108363963 cites W2145213569 @default.
- W2108363963 cites W2148148905 @default.
- W2108363963 cites W2148399857 @default.
- W2108363963 cites W2151126975 @default.
- W2108363963 cites W2157253543 @default.
- W2108363963 cites W2157471438 @default.
- W2108363963 cites W2158869723 @default.
- W2108363963 cites W2161335608 @default.
- W2108363963 cites W2161893527 @default.
- W2108363963 cites W2162253490 @default.
- W2108363963 cites W2163754862 @default.
- W2108363963 cites W2164516780 @default.
- W2108363963 cites W2172088410 @default.
- W2108363963 cites W2172097982 @default.
- W2108363963 cites W2419272096 @default.