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• W2108388081 abstract "ABSTRACT The expression of nitrogenase genes of Herbaspirillum sp. B501 associated in shoot (leaf and stem) of wild rice, Oryza officinalis , was studied by means of reverse transcription-PCR (RT-PCR) targeted at the nifH gene. RT-PCR analyses indicate that nifH transcript was detected exclusively from nitrogen-fixing cells of gfp -tagged strain B501 gfp 1 in both free-living and endophytic states by using a constitutive gfp gene transcript as a positive control. Transcription of nifH and nitrogen fixation in free-living cells were induced maximally at a 2% O 2 concentration and repressed in free air (21% O 2 ). nifH transcription was monitored in the endophytic cells by using total RNA extracted from B501 gfp 1-inoculated wild rice plants during daily light-dark cycles. The level of nifH transcription in planta varied dramatically, with a maximum during the light period. Moreover, the light radiation enhanced nifH expression even in free-living cells grown in culture. These results suggest that in planta nitrogen fixation by the endophyte shows a daily rhythm determined by the plant's light environment." @default.
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• W2108388081 date "2005-12-01" @default.
• W2108388081 modified "2023-09-23" @default.
• W2108388081 title "Expression of the <i>nifH</i> Gene of a <i>Herbaspirillum</i> Endophyte in Wild Rice Species: Daily Rhythm during the Light-Dark Cycle" @default.
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• W2108388081 doi "https://doi.org/10.1128/aem.71.12.8183-8190.2005" @default.
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