Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108415533> ?p ?o ?g. }
- W2108415533 abstract "Coffee somatic embryogenesis in liquid medium is a powerful alternative to other vegetative propagation techniques for mass propagation of selected Coffea canephora (Robusta) clones and F1 Coffea arabica hybrids. This review presents the different types of bioreactors used for coffee somatic embryogenesis by Nestle R&D Centre-Tours and by other scientific teams. Mechanically agitated bioreactors were used for the production of torpedo-shaped embryos. Critical parameters are the inoculation density (0.5 to 1.0 g FW L -1 ), medium renewing and the initial oxygen transfer rate (KLa: 5 h -1 ). In this system, Robusta embryo concentrations range between 200,000 to 400,000 L -1 within 2 months. Maturation from the torpedo to the cotyledonary-stage embryos was achieved in various temporary immersion bioreactors (TIB): in 1-L RITA ® system (up to 1,000 cotyledonary embryos per system), in 10-L glass bottles (up to 20,000) and in 10-L flexible disposable bags. The latter one, the so-called “Box-in-Bags”, insures a higher light transmittance to the biomass due to its horizontal design. At the end of the maturation phase, the somatic embryos are green and able to photosynthesize: these pregerminated embryos can be directly transplanted to the greenhouse to get fully germinated plantlets. More recently, a temporary root immersion bioreactor (TRI) has been described for the growth of individualized Arabusta plantlets in photoautautrophic conditions, i.e. in sugar-free medium with enriched CO2 and high light intensity. The pros and cons of these different bioreactors will be discussed considering how they can be integrated in a mass propagation process. We present a “state of the art” by describing a pilot scale process for the production of pregerminated Robusta embryos and some examples of diffusion of coffee selected genotypes. These last years, two major trends have been developed for industrial micropropagation: i) bulk-cultivation of small propagules in photomixotrophic conditions (with sugar) followed by their selection and transfer to the greenhouse for their conversion to plant, ii) production of singulated and fully developed plantlets in the laboratory under photoautotrophic conditions. Next development in coffee mass propagation by somatic embryogenesis will probably originate from the combination of these two approaches. The usage of the “micro-environment” method, combined with media releasing CO2, is well adapted for the ex vitro germination of coffee embryos. Particularly, this method can be a relevant alternative to the conventional one, consisting on insufflating CO2 in the culture rooms or in the greenhouses." @default.
- W2108415533 created "2016-06-24" @default.
- W2108415533 creator A5031148073 @default.
- W2108415533 date "2007-01-01" @default.
- W2108415533 modified "2023-09-25" @default.
- W2108415533 title "Bioreactors for Coffee Mass Propagation by Somatic Embryogenesis" @default.
- W2108415533 cites W130913900 @default.
- W2108415533 cites W1511323272 @default.
- W2108415533 cites W1515689665 @default.
- W2108415533 cites W153258265 @default.
- W2108415533 cites W1533329671 @default.
- W2108415533 cites W1580909556 @default.
- W2108415533 cites W1594916836 @default.
- W2108415533 cites W1779074067 @default.
- W2108415533 cites W1937353767 @default.
- W2108415533 cites W1971708045 @default.
- W2108415533 cites W1976466619 @default.
- W2108415533 cites W1977107503 @default.
- W2108415533 cites W1982059280 @default.
- W2108415533 cites W1986310640 @default.
- W2108415533 cites W1997657203 @default.
- W2108415533 cites W2011072395 @default.
- W2108415533 cites W2020050548 @default.
- W2108415533 cites W2024556021 @default.
- W2108415533 cites W2029430202 @default.
- W2108415533 cites W2034150093 @default.
- W2108415533 cites W2035685613 @default.
- W2108415533 cites W2043592111 @default.
- W2108415533 cites W2044727427 @default.
- W2108415533 cites W2047178839 @default.
- W2108415533 cites W2050133884 @default.
- W2108415533 cites W2054429156 @default.
- W2108415533 cites W2056195407 @default.
- W2108415533 cites W2060653805 @default.
- W2108415533 cites W2070957942 @default.
- W2108415533 cites W2072400552 @default.
- W2108415533 cites W2072776616 @default.
- W2108415533 cites W2072940613 @default.
- W2108415533 cites W2077545872 @default.
- W2108415533 cites W2103859529 @default.
- W2108415533 cites W2137099730 @default.
- W2108415533 cites W214987660 @default.
- W2108415533 cites W2163136165 @default.
- W2108415533 cites W2174431960 @default.
- W2108415533 cites W2176157243 @default.
- W2108415533 cites W2197753197 @default.
- W2108415533 cites W2219967254 @default.
- W2108415533 cites W2254126496 @default.
- W2108415533 cites W2275671616 @default.
- W2108415533 cites W2284708979 @default.
- W2108415533 cites W2298314152 @default.
- W2108415533 cites W2412332452 @default.
- W2108415533 cites W2463364668 @default.
- W2108415533 cites W2525985077 @default.
- W2108415533 cites W254092118 @default.
- W2108415533 cites W2589891748 @default.
- W2108415533 cites W2590146916 @default.
- W2108415533 cites W2603261049 @default.
- W2108415533 cites W307850230 @default.
- W2108415533 cites W341619931 @default.
- W2108415533 cites W347674122 @default.
- W2108415533 cites W591548504 @default.
- W2108415533 cites W627865954 @default.
- W2108415533 cites W89779050 @default.
- W2108415533 cites W1760041416 @default.
- W2108415533 hasPublicationYear "2007" @default.
- W2108415533 type Work @default.
- W2108415533 sameAs 2108415533 @default.
- W2108415533 citedByCount "8" @default.
- W2108415533 countsByYear W21084155332012 @default.
- W2108415533 countsByYear W21084155332013 @default.
- W2108415533 countsByYear W21084155332016 @default.
- W2108415533 countsByYear W21084155332017 @default.
- W2108415533 crossrefType "journal-article" @default.
- W2108415533 hasAuthorship W2108415533A5031148073 @default.
- W2108415533 hasConcept C100701293 @default.
- W2108415533 hasConcept C144027150 @default.
- W2108415533 hasConcept C148761745 @default.
- W2108415533 hasConcept C162324750 @default.
- W2108415533 hasConcept C168170006 @default.
- W2108415533 hasConcept C196843134 @default.
- W2108415533 hasConcept C201468095 @default.
- W2108415533 hasConcept C202751555 @default.
- W2108415533 hasConcept C2778597767 @default.
- W2108415533 hasConcept C2780822416 @default.
- W2108415533 hasConcept C2983132609 @default.
- W2108415533 hasConcept C31903555 @default.
- W2108415533 hasConcept C536366893 @default.
- W2108415533 hasConcept C55493867 @default.
- W2108415533 hasConcept C556758197 @default.
- W2108415533 hasConcept C59822182 @default.
- W2108415533 hasConcept C86803240 @default.
- W2108415533 hasConcept C87073359 @default.
- W2108415533 hasConcept C90703135 @default.
- W2108415533 hasConcept C95444343 @default.
- W2108415533 hasConceptScore W2108415533C100701293 @default.
- W2108415533 hasConceptScore W2108415533C144027150 @default.
- W2108415533 hasConceptScore W2108415533C148761745 @default.
- W2108415533 hasConceptScore W2108415533C162324750 @default.
- W2108415533 hasConceptScore W2108415533C168170006 @default.