Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108422451> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2108422451 endingPage "207a" @default.
- W2108422451 startingPage "207a" @default.
- W2108422451 abstract "In order to infect human cells, pathogenic Gram-negative bacteria depend on their ability to secrete toxins into the cytoplasm of an adjacent eukaryotic host cell. A conserved and efficient delivery mechanism, called the type III secretion (T3S) system, acts as a syringe to inject these toxic proteins called “effectors” which need to translocate through the eukaryotic plasma membrane. In Pseudomonas aeruginosa PopB and PopD are essential for effector translocation. Substantial genetic and biochemical data support a model with PopB and PopD inserting into the target membrane and forming an oligomeric translocon pore, however virtually nothing is known about this critical transmembrane complex. Our primary goal is to determine the arrangement of PopD in membrane assembled translocons. What segments of PopD interact with the membrane? Does PopB influence the PopD/membrane interaction? Using membrane binding assays, conductivity measures in planar lipid bilayers, site-directed fluorescence labeling, and multiple fluorescence approaches we have analyzed the interaction of PopD with model membranes and the pore formation activity of the translocator in the presence and absence of PopB. Despite both proteins can individually form pores in membranes, we found that PopD oligomerizes with PopB and forms pores with an inner diameter of approximately 30Å. Our analysis also showed that the PopD predicted transmembrane segment (residues L119-V137), does not adopt a transmembrane orientation. Our data support a model that consists in the binding of the unfolded translocators to the membrane, oligomerization, and formation of discrete transmembrane pores." @default.
- W2108422451 created "2016-06-24" @default.
- W2108422451 creator A5012718640 @default.
- W2108422451 creator A5048266925 @default.
- W2108422451 creator A5051480095 @default.
- W2108422451 creator A5056897795 @default.
- W2108422451 date "2011-02-01" @default.
- W2108422451 modified "2023-09-26" @default.
- W2108422451 title "Insights into the Membrane-Interaction Properties of the PSEUDOMONAS Aeruginosa Type III Secretion Translocator Popd" @default.
- W2108422451 doi "https://doi.org/10.1016/j.bpj.2010.12.1344" @default.
- W2108422451 hasPublicationYear "2011" @default.
- W2108422451 type Work @default.
- W2108422451 sameAs 2108422451 @default.
- W2108422451 citedByCount "0" @default.
- W2108422451 crossrefType "journal-article" @default.
- W2108422451 hasAuthorship W2108422451A5012718640 @default.
- W2108422451 hasAuthorship W2108422451A5048266925 @default.
- W2108422451 hasAuthorship W2108422451A5051480095 @default.
- W2108422451 hasAuthorship W2108422451A5056897795 @default.
- W2108422451 hasBestOaLocation W21084224511 @default.
- W2108422451 hasConcept C118892022 @default.
- W2108422451 hasConcept C12554922 @default.
- W2108422451 hasConcept C144647389 @default.
- W2108422451 hasConcept C170493617 @default.
- W2108422451 hasConcept C185592680 @default.
- W2108422451 hasConcept C190062978 @default.
- W2108422451 hasConcept C199229279 @default.
- W2108422451 hasConcept C24530287 @default.
- W2108422451 hasConcept C41625074 @default.
- W2108422451 hasConcept C49039625 @default.
- W2108422451 hasConcept C51785407 @default.
- W2108422451 hasConcept C55493867 @default.
- W2108422451 hasConcept C86803240 @default.
- W2108422451 hasConcept C95444343 @default.
- W2108422451 hasConceptScore W2108422451C118892022 @default.
- W2108422451 hasConceptScore W2108422451C12554922 @default.
- W2108422451 hasConceptScore W2108422451C144647389 @default.
- W2108422451 hasConceptScore W2108422451C170493617 @default.
- W2108422451 hasConceptScore W2108422451C185592680 @default.
- W2108422451 hasConceptScore W2108422451C190062978 @default.
- W2108422451 hasConceptScore W2108422451C199229279 @default.
- W2108422451 hasConceptScore W2108422451C24530287 @default.
- W2108422451 hasConceptScore W2108422451C41625074 @default.
- W2108422451 hasConceptScore W2108422451C49039625 @default.
- W2108422451 hasConceptScore W2108422451C51785407 @default.
- W2108422451 hasConceptScore W2108422451C55493867 @default.
- W2108422451 hasConceptScore W2108422451C86803240 @default.
- W2108422451 hasConceptScore W2108422451C95444343 @default.
- W2108422451 hasIssue "3" @default.
- W2108422451 hasLocation W21084224511 @default.
- W2108422451 hasOpenAccess W2108422451 @default.
- W2108422451 hasPrimaryLocation W21084224511 @default.
- W2108422451 hasRelatedWork W2013490788 @default.
- W2108422451 hasRelatedWork W2030239940 @default.
- W2108422451 hasRelatedWork W2038131958 @default.
- W2108422451 hasRelatedWork W2067732567 @default.
- W2108422451 hasRelatedWork W2108422451 @default.
- W2108422451 hasRelatedWork W2163812219 @default.
- W2108422451 hasRelatedWork W2564117472 @default.
- W2108422451 hasRelatedWork W2888490003 @default.
- W2108422451 hasRelatedWork W4245378367 @default.
- W2108422451 hasRelatedWork W4320723860 @default.
- W2108422451 hasVolume "100" @default.
- W2108422451 isParatext "false" @default.
- W2108422451 isRetracted "false" @default.
- W2108422451 magId "2108422451" @default.
- W2108422451 workType "article" @default.